F Saemi, A Shirazi Nezhad, M H Hosseini, M Hashemi, A Rahimian, M Kamgar, R Rezaeishoorijeh, F Dabiri, M Hayati, S Amiri
{"title":"鸡油佐剂灭活大肠埃希菌疫苗对O78型禽大肠埃希菌感染的体液免疫效果评价","authors":"F Saemi, A Shirazi Nezhad, M H Hosseini, M Hashemi, A Rahimian, M Kamgar, R Rezaeishoorijeh, F Dabiri, M Hayati, S Amiri","doi":"10.32592/ARI.2025.80.1.161","DOIUrl":null,"url":null,"abstract":"<p><p>Colibacillosis, a major bacterial disease affecting chickens and turkeys, is caused by avian pathogenic Escherichia coli (APEC). The disease has significant economic implications for poultry farms, resulting in increased mortality, reduced body weight, and higher feed conversion ratios (FCRs). These factors can lead to higher carcass condemnation at slaughterhouses.In recent years, significant progress has been made in the development of vaccines against APEC, including both homologous and heterologous vaccines. The present study employed mineral oil as an adjuvant for inactivated E. coli, which was inoculated via injection route to layer chickens.At 28 days of age, 60 birds were subsequently divided into six experimental groups of 10 chickens per group. The control group did not receive the E. coli vaccine, whereas the five treatment groups were vaccinated subcutaneously with a formalin-inactivated, mineral-oil adjuvant E. coli vaccine containing an isolate of E. coli serotype O<sub>78</sub>. The T1, T2, T3, T4, and T5 groups were vaccinated at The T1, T2, T3, T4 and T5 groups were inoculated at 28 days of age with 0.2 ml (8 ×10<sup>6</sup>, 16 ×10<sup>6</sup>, 33 ×10<sup>6</sup>, 66 ×10<sup>6</sup> and 133 ×10<sup>6</sup> cfu/ml) of E. coli O<sub>78</sub>, respectively.Ten weeks after inoculation, the levels of IgG antibody titres against E. coli were evaluated using an ELISA method. The results demonstrated a significant increase in IgG antibody titres in the immunised birds compared to the unimmunised control group (P <0.05). Anti-IgG antibodies increased on a weekly basis after injection in most vaccinated groups up to four weeks.In conclusion, the prepared E. coli vaccine at the Razi Institute, Shiraz branch, induced high levels of immune responses in the vaccinated group, as revealed by ELISA. In order to elicit a substantial immunological stimulus, it is recommended that all chickens in the experimental group receive a booster dose four weeks after the initial immunization.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"80 1","pages":"161-168"},"PeriodicalIF":0.0000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12426440/pdf/","citationCount":"0","resultStr":"{\"title\":\"Evaluation of the Efficacy of Humoral Immunity Response of Killed Oil Adjuvant <i>Escherichia coli</i> Vaccine in Layer Chicken against Avian <i>E. coli</i> Serotype O<sub>78</sub> Infection.\",\"authors\":\"F Saemi, A Shirazi Nezhad, M H Hosseini, M Hashemi, A Rahimian, M Kamgar, R Rezaeishoorijeh, F Dabiri, M Hayati, S Amiri\",\"doi\":\"10.32592/ARI.2025.80.1.161\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Colibacillosis, a major bacterial disease affecting chickens and turkeys, is caused by avian pathogenic Escherichia coli (APEC). The disease has significant economic implications for poultry farms, resulting in increased mortality, reduced body weight, and higher feed conversion ratios (FCRs). These factors can lead to higher carcass condemnation at slaughterhouses.In recent years, significant progress has been made in the development of vaccines against APEC, including both homologous and heterologous vaccines. The present study employed mineral oil as an adjuvant for inactivated E. coli, which was inoculated via injection route to layer chickens.At 28 days of age, 60 birds were subsequently divided into six experimental groups of 10 chickens per group. The control group did not receive the E. coli vaccine, whereas the five treatment groups were vaccinated subcutaneously with a formalin-inactivated, mineral-oil adjuvant E. coli vaccine containing an isolate of E. coli serotype O<sub>78</sub>. The T1, T2, T3, T4, and T5 groups were vaccinated at The T1, T2, T3, T4 and T5 groups were inoculated at 28 days of age with 0.2 ml (8 ×10<sup>6</sup>, 16 ×10<sup>6</sup>, 33 ×10<sup>6</sup>, 66 ×10<sup>6</sup> and 133 ×10<sup>6</sup> cfu/ml) of E. coli O<sub>78</sub>, respectively.Ten weeks after inoculation, the levels of IgG antibody titres against E. coli were evaluated using an ELISA method. The results demonstrated a significant increase in IgG antibody titres in the immunised birds compared to the unimmunised control group (P <0.05). Anti-IgG antibodies increased on a weekly basis after injection in most vaccinated groups up to four weeks.In conclusion, the prepared E. coli vaccine at the Razi Institute, Shiraz branch, induced high levels of immune responses in the vaccinated group, as revealed by ELISA. 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引用次数: 0
摘要
大肠杆菌病是一种影响鸡和火鸡的主要细菌性疾病,由禽致病性大肠杆菌(APEC)引起。该病对家禽养殖场具有重大的经济影响,导致死亡率增加、体重减轻和饲料转化率(fcr)提高。这些因素会导致屠宰场对胴体的更高谴责。近年来,亚太经合组织疫苗的研制取得了重大进展,包括同源疫苗和异源疫苗。本研究采用矿物油作为灭活大肠杆菌的佐剂,通过注射方式接种于蛋鸡。28日龄时,将60只鸡分为6个试验组,每组10只鸡。对照组未接种大肠杆菌疫苗,而五个治疗组则皮下接种含有O78型大肠杆菌分离株的福尔马林灭活矿物油佐剂大肠杆菌疫苗。T1、T2、T3、T4和T5组在28日龄时分别接种0.2 ml (8 ×106、16 ×106、33 ×106、66 ×106和133 ×106 cfu/ml)的大肠杆菌O78。接种10周后,用ELISA法测定抗大肠杆菌IgG抗体滴度。结果表明,与未免疫的对照组相比,免疫组的IgG抗体滴度显著增加(P
Evaluation of the Efficacy of Humoral Immunity Response of Killed Oil Adjuvant Escherichia coli Vaccine in Layer Chicken against Avian E. coli Serotype O78 Infection.
Colibacillosis, a major bacterial disease affecting chickens and turkeys, is caused by avian pathogenic Escherichia coli (APEC). The disease has significant economic implications for poultry farms, resulting in increased mortality, reduced body weight, and higher feed conversion ratios (FCRs). These factors can lead to higher carcass condemnation at slaughterhouses.In recent years, significant progress has been made in the development of vaccines against APEC, including both homologous and heterologous vaccines. The present study employed mineral oil as an adjuvant for inactivated E. coli, which was inoculated via injection route to layer chickens.At 28 days of age, 60 birds were subsequently divided into six experimental groups of 10 chickens per group. The control group did not receive the E. coli vaccine, whereas the five treatment groups were vaccinated subcutaneously with a formalin-inactivated, mineral-oil adjuvant E. coli vaccine containing an isolate of E. coli serotype O78. The T1, T2, T3, T4, and T5 groups were vaccinated at The T1, T2, T3, T4 and T5 groups were inoculated at 28 days of age with 0.2 ml (8 ×106, 16 ×106, 33 ×106, 66 ×106 and 133 ×106 cfu/ml) of E. coli O78, respectively.Ten weeks after inoculation, the levels of IgG antibody titres against E. coli were evaluated using an ELISA method. The results demonstrated a significant increase in IgG antibody titres in the immunised birds compared to the unimmunised control group (P <0.05). Anti-IgG antibodies increased on a weekly basis after injection in most vaccinated groups up to four weeks.In conclusion, the prepared E. coli vaccine at the Razi Institute, Shiraz branch, induced high levels of immune responses in the vaccinated group, as revealed by ELISA. In order to elicit a substantial immunological stimulus, it is recommended that all chickens in the experimental group receive a booster dose four weeks after the initial immunization.