{"title":"适应热带沿海气候的土生牛HSP90AA1基因的遗传多态性","authors":"Kumudinee Shivankar , Amiya Ranjan Sahu , Gokuldas P.P , Nibedita Nayak , Veeranna Gowda","doi":"10.1016/j.jtherbio.2025.104273","DOIUrl":null,"url":null,"abstract":"<div><div>Heat stress proteins are important factors in protecting the cells of animals and humans against environmental stress, leading to cellular homeostasis. Zebu cattle are well known for the prevalence of novel mutations responsible for heat tolerance. Hence, the study was carried out to identify single nucleotide polymorphisms in the heat shock protein gene (HSP90AA1) in Shweta Kapila, Sahiwal, Gir and Malnad Gidda cattle breeds adapted to hot and humid coastal climate. Blood samples were collected and genomic DNA amplified for HSP90AA1 gene using designed oligonucleotide primers and sequenced. Rectal temperature and respiration rate both were observed elevated during the hot period compared to the cold period. Sequence data of all the amplified regions of HSP90 gene was analyzed using EditSeq and SeqMan of LASERGENE software. The analysis of custom sequencing data unveiled the presence of ten novel Single Nucleotide Polymorphisms SNPs) located at g.G4733C, g.C4765A and g.A4848G in 3′ UTR; g.C1012T and g.A1209G in exon 3; g.C1300T in intron 3; g.C2245T and g.T2266G in exon 5; and g.T3814A and g.G4212T in exon 10. The identified SNPs were genotyped by PCR-RFLP (<em>Aci</em>I and <em>Hpy</em>CH4III enzymes) and Tetra-primer ARMS-PCR. Genotypic and allelic frequencies for the regions ranged between 0.094 and 0.760, and 0.2645 to 0.7355, respectively. This was the novel report of genetic variations in the HSP90AA1 gene in selected indigenous cattle breeds of India adapted to hot and humid coastal climates. Further studies with large sample sizes are needed to genotype the loci for their significant effect on thermotolerance ability in cattle.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"133 ","pages":"Article 104273"},"PeriodicalIF":2.9000,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic polymorphism in the HSP90AA1 gene revealed in indigenous cattle adapted to tropical coastal climate\",\"authors\":\"Kumudinee Shivankar , Amiya Ranjan Sahu , Gokuldas P.P , Nibedita Nayak , Veeranna Gowda\",\"doi\":\"10.1016/j.jtherbio.2025.104273\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Heat stress proteins are important factors in protecting the cells of animals and humans against environmental stress, leading to cellular homeostasis. Zebu cattle are well known for the prevalence of novel mutations responsible for heat tolerance. Hence, the study was carried out to identify single nucleotide polymorphisms in the heat shock protein gene (HSP90AA1) in Shweta Kapila, Sahiwal, Gir and Malnad Gidda cattle breeds adapted to hot and humid coastal climate. Blood samples were collected and genomic DNA amplified for HSP90AA1 gene using designed oligonucleotide primers and sequenced. Rectal temperature and respiration rate both were observed elevated during the hot period compared to the cold period. Sequence data of all the amplified regions of HSP90 gene was analyzed using EditSeq and SeqMan of LASERGENE software. The analysis of custom sequencing data unveiled the presence of ten novel Single Nucleotide Polymorphisms SNPs) located at g.G4733C, g.C4765A and g.A4848G in 3′ UTR; g.C1012T and g.A1209G in exon 3; g.C1300T in intron 3; g.C2245T and g.T2266G in exon 5; and g.T3814A and g.G4212T in exon 10. The identified SNPs were genotyped by PCR-RFLP (<em>Aci</em>I and <em>Hpy</em>CH4III enzymes) and Tetra-primer ARMS-PCR. Genotypic and allelic frequencies for the regions ranged between 0.094 and 0.760, and 0.2645 to 0.7355, respectively. This was the novel report of genetic variations in the HSP90AA1 gene in selected indigenous cattle breeds of India adapted to hot and humid coastal climates. Further studies with large sample sizes are needed to genotype the loci for their significant effect on thermotolerance ability in cattle.</div></div>\",\"PeriodicalId\":17428,\"journal\":{\"name\":\"Journal of thermal biology\",\"volume\":\"133 \",\"pages\":\"Article 104273\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of thermal biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S030645652500230X\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of thermal biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S030645652500230X","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOLOGY","Score":null,"Total":0}
Genetic polymorphism in the HSP90AA1 gene revealed in indigenous cattle adapted to tropical coastal climate
Heat stress proteins are important factors in protecting the cells of animals and humans against environmental stress, leading to cellular homeostasis. Zebu cattle are well known for the prevalence of novel mutations responsible for heat tolerance. Hence, the study was carried out to identify single nucleotide polymorphisms in the heat shock protein gene (HSP90AA1) in Shweta Kapila, Sahiwal, Gir and Malnad Gidda cattle breeds adapted to hot and humid coastal climate. Blood samples were collected and genomic DNA amplified for HSP90AA1 gene using designed oligonucleotide primers and sequenced. Rectal temperature and respiration rate both were observed elevated during the hot period compared to the cold period. Sequence data of all the amplified regions of HSP90 gene was analyzed using EditSeq and SeqMan of LASERGENE software. The analysis of custom sequencing data unveiled the presence of ten novel Single Nucleotide Polymorphisms SNPs) located at g.G4733C, g.C4765A and g.A4848G in 3′ UTR; g.C1012T and g.A1209G in exon 3; g.C1300T in intron 3; g.C2245T and g.T2266G in exon 5; and g.T3814A and g.G4212T in exon 10. The identified SNPs were genotyped by PCR-RFLP (AciI and HpyCH4III enzymes) and Tetra-primer ARMS-PCR. Genotypic and allelic frequencies for the regions ranged between 0.094 and 0.760, and 0.2645 to 0.7355, respectively. This was the novel report of genetic variations in the HSP90AA1 gene in selected indigenous cattle breeds of India adapted to hot and humid coastal climates. Further studies with large sample sizes are needed to genotype the loci for their significant effect on thermotolerance ability in cattle.
期刊介绍:
The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are:
• The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature
• The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature
• Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause
• Effects of temperature on reproduction and development, growth, ageing and life-span
• Studies on modelling heat transfer between organisms and their environment
• The contributions of temperature to effects of climate change on animal species and man
• Studies of conservation biology and physiology related to temperature
• Behavioural and physiological regulation of body temperature including its pathophysiology and fever
• Medical applications of hypo- and hyperthermia
Article types:
• Original articles
• Review articles