Xiexuan Wang, Lingxia Chen, Lindong Dou, Jinhui Yu, Xueqian Wang, Satoru Kondo, Takanori Saito, ZiXiao Wu, Jie Denga, Bing Jia, Zhenfeng Ye, Lun Liu, Xiaomei Tang, Li Liu, Wei Heng
{"title":"‘砀山苏里’赤褐色突变体PbrMYB31-PbrbHLH147复合物活性对ABA的响应促进了脂肪酸的生物合成和沉积","authors":"Xiexuan Wang, Lingxia Chen, Lindong Dou, Jinhui Yu, Xueqian Wang, Satoru Kondo, Takanori Saito, ZiXiao Wu, Jie Denga, Bing Jia, Zhenfeng Ye, Lun Liu, Xiaomei Tang, Li Liu, Wei Heng","doi":"10.1016/j.hpj.2025.05.014","DOIUrl":null,"url":null,"abstract":"Suberin is a naturally occurring polymer found in plant cell walls that plays crucial roles in protecting plants from environmental stress and enhancing resistance to pathogens. Despite its biological importance, the molecular regulatory networks governing suberin biosynthesis are not fully understood. In the russet pericarp of ‘Dangshanjinsu’ pear, suberin accumulation, which is enriched with very long-chain fatty acids, contributes to the formation of a robust protective barrier. Transcriptome data analysis revealed that PbrMYB31 and PbrbHLH147 were the key differentially expressed transcription factors in the pericarp of both ‘Dangshansuli’ and ‘Dangshanjinsu’ at various developmental stages. In this study, overexpression of <ce:italic>PbrMYB31</ce:italic> and <ce:italic>PbrbHLH147</ce:italic> was found to positively regulate long-chain fatty acid synthesis, promote suberin formation, and increase sensitivity to abscisic acid (ABA). Additionally, PbrMYB31 interacts with PbrbHLH147, forming a regulatory complex that synergistically extends the carbon chain length of fatty acids, a critical step in suberin assembly. ABA signaling was shown to integrate into this pathway, further modulating suberin production and linking hormonal regulation with structural barrier formation. This study provides novel insights into the genetic and hormonal control of suberin biosynthesis in russeted pear pericarp, emphasizing its critical role in enhancing fruit protection, improving quality, and extending shelf life, thereby contributing to advancements in horticultural science and sustainable fruit production.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"16 1","pages":""},"PeriodicalIF":6.2000,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"PbrMYB31–PbrbHLH147 complex activity in response to ABA promotes fatty acid biosynthesis for suberin deposition in the russet mutant of ‘Dangshansuli’\",\"authors\":\"Xiexuan Wang, Lingxia Chen, Lindong Dou, Jinhui Yu, Xueqian Wang, Satoru Kondo, Takanori Saito, ZiXiao Wu, Jie Denga, Bing Jia, Zhenfeng Ye, Lun Liu, Xiaomei Tang, Li Liu, Wei Heng\",\"doi\":\"10.1016/j.hpj.2025.05.014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Suberin is a naturally occurring polymer found in plant cell walls that plays crucial roles in protecting plants from environmental stress and enhancing resistance to pathogens. Despite its biological importance, the molecular regulatory networks governing suberin biosynthesis are not fully understood. In the russet pericarp of ‘Dangshanjinsu’ pear, suberin accumulation, which is enriched with very long-chain fatty acids, contributes to the formation of a robust protective barrier. Transcriptome data analysis revealed that PbrMYB31 and PbrbHLH147 were the key differentially expressed transcription factors in the pericarp of both ‘Dangshansuli’ and ‘Dangshanjinsu’ at various developmental stages. In this study, overexpression of <ce:italic>PbrMYB31</ce:italic> and <ce:italic>PbrbHLH147</ce:italic> was found to positively regulate long-chain fatty acid synthesis, promote suberin formation, and increase sensitivity to abscisic acid (ABA). Additionally, PbrMYB31 interacts with PbrbHLH147, forming a regulatory complex that synergistically extends the carbon chain length of fatty acids, a critical step in suberin assembly. ABA signaling was shown to integrate into this pathway, further modulating suberin production and linking hormonal regulation with structural barrier formation. This study provides novel insights into the genetic and hormonal control of suberin biosynthesis in russeted pear pericarp, emphasizing its critical role in enhancing fruit protection, improving quality, and extending shelf life, thereby contributing to advancements in horticultural science and sustainable fruit production.\",\"PeriodicalId\":13178,\"journal\":{\"name\":\"Horticultural Plant Journal\",\"volume\":\"16 1\",\"pages\":\"\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2025-09-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Horticultural Plant Journal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.hpj.2025.05.014\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticultural Plant Journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.hpj.2025.05.014","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
PbrMYB31–PbrbHLH147 complex activity in response to ABA promotes fatty acid biosynthesis for suberin deposition in the russet mutant of ‘Dangshansuli’
Suberin is a naturally occurring polymer found in plant cell walls that plays crucial roles in protecting plants from environmental stress and enhancing resistance to pathogens. Despite its biological importance, the molecular regulatory networks governing suberin biosynthesis are not fully understood. In the russet pericarp of ‘Dangshanjinsu’ pear, suberin accumulation, which is enriched with very long-chain fatty acids, contributes to the formation of a robust protective barrier. Transcriptome data analysis revealed that PbrMYB31 and PbrbHLH147 were the key differentially expressed transcription factors in the pericarp of both ‘Dangshansuli’ and ‘Dangshanjinsu’ at various developmental stages. In this study, overexpression of PbrMYB31 and PbrbHLH147 was found to positively regulate long-chain fatty acid synthesis, promote suberin formation, and increase sensitivity to abscisic acid (ABA). Additionally, PbrMYB31 interacts with PbrbHLH147, forming a regulatory complex that synergistically extends the carbon chain length of fatty acids, a critical step in suberin assembly. ABA signaling was shown to integrate into this pathway, further modulating suberin production and linking hormonal regulation with structural barrier formation. This study provides novel insights into the genetic and hormonal control of suberin biosynthesis in russeted pear pericarp, emphasizing its critical role in enhancing fruit protection, improving quality, and extending shelf life, thereby contributing to advancements in horticultural science and sustainable fruit production.
期刊介绍:
Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.