Jacqueline R Salazar, Claudia Gonzalez, Oris Chavarría, Tybbysay P Salinas, Publio González, Blas Armién, Alexander Martinez
{"title":"利用平铺聚合酶链反应对巧克力原汉坦病毒进行全基因组测序和基因组鉴定。","authors":"Jacqueline R Salazar, Claudia Gonzalez, Oris Chavarría, Tybbysay P Salinas, Publio González, Blas Armién, Alexander Martinez","doi":"10.4269/ajtmh.24-0813","DOIUrl":null,"url":null,"abstract":"<p><p>In the present study, a tiled amplification protocol was developed to cover the three segments of Choclo virus (CHOV), which was subsequently used for sequencing samples from different provinces within the endemic zone in Panama. The developed protocol yielded a coverage of the CHOV genome greater than 99% for most of the sample's segments, and the four consensus sequences obtained had a mean coverage of 94-99%. Generating a significant number of whole-genome sequences is necessary for studying genomic characteristics among CHOV isolated from rodent hosts, as well as those isolated from human samples. Employing enhanced methodology for whole-genome consensus facilitates the validation and advancement of cutting-edge detection methods, while also providing valuable insights for comprehensive studies on CHOV.</p>","PeriodicalId":7752,"journal":{"name":"American Journal of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Complete Genome Sequencing and Genomic Characterization of Orthohantavirus chocloense Using Tiled Polymerase Chain Reaction.\",\"authors\":\"Jacqueline R Salazar, Claudia Gonzalez, Oris Chavarría, Tybbysay P Salinas, Publio González, Blas Armién, Alexander Martinez\",\"doi\":\"10.4269/ajtmh.24-0813\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In the present study, a tiled amplification protocol was developed to cover the three segments of Choclo virus (CHOV), which was subsequently used for sequencing samples from different provinces within the endemic zone in Panama. The developed protocol yielded a coverage of the CHOV genome greater than 99% for most of the sample's segments, and the four consensus sequences obtained had a mean coverage of 94-99%. Generating a significant number of whole-genome sequences is necessary for studying genomic characteristics among CHOV isolated from rodent hosts, as well as those isolated from human samples. Employing enhanced methodology for whole-genome consensus facilitates the validation and advancement of cutting-edge detection methods, while also providing valuable insights for comprehensive studies on CHOV.</p>\",\"PeriodicalId\":7752,\"journal\":{\"name\":\"American Journal of Tropical Medicine and Hygiene\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-09-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American Journal of Tropical Medicine and Hygiene\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.4269/ajtmh.24-0813\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American Journal of Tropical Medicine and Hygiene","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4269/ajtmh.24-0813","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
Complete Genome Sequencing and Genomic Characterization of Orthohantavirus chocloense Using Tiled Polymerase Chain Reaction.
In the present study, a tiled amplification protocol was developed to cover the three segments of Choclo virus (CHOV), which was subsequently used for sequencing samples from different provinces within the endemic zone in Panama. The developed protocol yielded a coverage of the CHOV genome greater than 99% for most of the sample's segments, and the four consensus sequences obtained had a mean coverage of 94-99%. Generating a significant number of whole-genome sequences is necessary for studying genomic characteristics among CHOV isolated from rodent hosts, as well as those isolated from human samples. Employing enhanced methodology for whole-genome consensus facilitates the validation and advancement of cutting-edge detection methods, while also providing valuable insights for comprehensive studies on CHOV.
期刊介绍：
The American Journal of Tropical Medicine and Hygiene, established in 1921, is published monthly by the American Society of Tropical Medicine and Hygiene. It is among the top-ranked tropical medicine journals in the world publishing original scientific articles and the latest science covering new research with an emphasis on population, clinical and laboratory science and the application of technology in the fields of tropical medicine, parasitology, immunology, infectious diseases, epidemiology, basic and molecular biology, virology and international medicine.
The Journal publishes unsolicited peer-reviewed manuscripts, review articles, short reports, images in Clinical Tropical Medicine, case studies, reports on the efficacy of new drugs and methods of treatment, prevention and control methodologies,new testing methods and equipment, book reports and Letters to the Editor. Topics range from applied epidemiology in such relevant areas as AIDS to the molecular biology of vaccine development.
The Journal is of interest to epidemiologists, parasitologists, virologists, clinicians, entomologists and public health officials who are concerned with health issues of the tropics, developing nations and emerging infectious diseases. Major granting institutions including philanthropic and governmental institutions active in the public health field, and medical and scientific libraries throughout the world purchase the Journal.
Two or more supplements to the Journal on topics of special interest are published annually. These supplements represent comprehensive and multidisciplinary discussions of issues of concern to tropical disease specialists and health issues of developing countries
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
