RNA结合基序39 （RBM39）串联RNA识别基序（RRM1-2）的1H， 13C和15N骨干共振分配。

IF 0.6 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2025-09-13 DOI:10.1007/s12104-025-10248-z

Florian Malard, Léa Bouton, Sébastien Campagne

{"title":"RNA结合基序39 （RBM39）串联RNA识别基序（RRM1-2）的1H， 13C和15N骨干共振分配。","authors":"Florian Malard, Léa Bouton, Sébastien Campagne","doi":"10.1007/s12104-025-10248-z","DOIUrl":null,"url":null,"abstract":"The N-terminal tandem RNA Recognition Motifs (RRM1-2) of the splicing factor RNA Binding Motif 39 (RBM39) from Homo sapiens was expressed in E. coli with 13C and 15N labeling. A nearly complete assignment of backbone 1H, 15N, and 13C resonances was obtained, along with a secondary structure prediction based on the assigned chemical shifts. The predicted secondary structures of the RBM39 tandem RRM1-2 closely matched the published solution structures of the individual RBM39 RRM1 and RRM2, and also aligned well with the AlphaFold2 model of RBM39.","PeriodicalId":492,"journal":{"name":"Biomolecular NMR Assignments","volume":" ","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"1H, 13C and 15N backbone resonance assignment of the RNA binding motif 39 (RBM39) tandem RNA recognition motifs (RRM1-2).\",\"authors\":\"Florian Malard, Léa Bouton, Sébastien Campagne\",\"doi\":\"10.1007/s12104-025-10248-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The N-terminal tandem RNA Recognition Motifs (RRM1-2) of the splicing factor RNA Binding Motif 39 (RBM39) from Homo sapiens was expressed in E. coli with 13C and 15N labeling. A nearly complete assignment of backbone 1H, 15N, and 13C resonances was obtained, along with a secondary structure prediction based on the assigned chemical shifts. The predicted secondary structures of the RBM39 tandem RRM1-2 closely matched the published solution structures of the individual RBM39 RRM1 and RRM2, and also aligned well with the AlphaFold2 model of RBM39.\",\"PeriodicalId\":492,\"journal\":{\"name\":\"Biomolecular NMR Assignments\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2025-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomolecular NMR Assignments\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s12104-025-10248-z\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomolecular NMR Assignments","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12104-025-10248-z","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

摘要

用13C和15N标记在大肠杆菌中表达了来自人类的剪接因子RNA结合Motif 39 （RBM39）的n端串联RNA识别Motif （RRM1-2）。获得了几乎完整的主链1H、15N和13C共振，以及基于分配的化学位移的二级结构预测。预测的RBM39串联RRM1-2的二级结构与已发表的单个RBM39 RRM1和RRM2的溶液结构非常吻合，并且与RBM39的AlphaFold2模型吻合良好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

¹H, ¹³C and ¹⁵N backbone resonance assignment of the RNA binding motif 39 (RBM39) tandem RNA recognition motifs (RRM1-2).

The N-terminal tandem RNA Recognition Motifs (RRM1-2) of the splicing factor RNA Binding Motif 39 (RBM39) from Homo sapiens was expressed in E. coli with ¹³C and ¹⁵N labeling. A nearly complete assignment of backbone ¹H, ¹⁵N, and ¹³C resonances was obtained, along with a secondary structure prediction based on the assigned chemical shifts. The predicted secondary structures of the RBM39 tandem RRM1-2 closely matched the published solution structures of the individual RBM39 RRM1 and RRM2, and also aligned well with the AlphaFold2 model of RBM39.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.