利用枯草芽孢杆菌SNF-1对含硼和非含硼304 L不锈钢的生物腐蚀研究

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-09-09 DOI:10.1016/j.bioelechem.2025.109106

Namrata Upadhyay , Sudhir K. Shukla , N. Malathy , Y.V. Nancharaiah , A. Ravi Shankar , S. Ningshen

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引用次数: 0

摘要

本研究利用从乏燃料池（SNF）中分离出来的枯草芽孢杆菌SNF-1研究了硼化和非硼化304 L不锈钢的微生物影响腐蚀（MIC）问题。在28天的时间里，电化学分析显示了不同的腐蚀行为：与未含硼的304 L SS（从0.05到- 0.10 V）相比，含硼的304 L SS的开路电位（从0.03到- 0.35 V vs Ag/AgCl）下降更为明显，表明对MIC的敏感性更高。动电位极化研究表明，无源电流密度增加（未加硼的304 L SS从1.5 μA.cm−2增加到2.4 μA.cm−2，加硼的304 L SS从2.4 μA.cm−2增加到3.4 μA.cm−2），同时点蚀电位降低，表明枯草芽孢杆菌SNF-1在MIC中的作用。电化学阻抗谱证实了加速降解，含硼304 L SS的极化电阻（Rp）下降了69%，未含硼304 L SS的极化电阻（Rp）下降了86%。尽管含硼304 L SS的绝对腐蚀速率更高，但由于生物膜覆盖率更高（95%对74%），未含硼304 L SS的腐蚀相对增加更大（3.8倍对2.3倍）。表面分析确定了生物膜下的局部点蚀，由硼化物引起的微电效应加剧。这些发现强调了合金微观结构和生物膜动力学在MIC严重程度中的双重作用。

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Biocorrosion studies on borated and non-borated 304 L stainless steel using Bacillus subtilis SNF-1, a bacterial isolate from SNF pool

This study investigates microbiologically-influenced corrosion (MIC) aspects of borated and non-borated 304 L- stainless-steel using Bacillus subtilis SNF-1, which was isolated from the spent nuclear fuel pool (SNF). Over 28 days, electrochemical analyses revealed distinct corrosion behaviours: borated 304 L SS exhibited a more pronounced decrease in open circuit potential (from 0.03 to −0.35 V vs. Ag/AgCl) as compared to non-borated 304 L SS (from 0.05 to −0.10 V vs. Ag/AgCl) indicating higher susceptibility to MIC. Potentiodynamic polarization studies revealed an increase in passive current density (from 1.5 to 2.4 μA.cm⁻² for non-borated 304 L SS and from 2.4 to 3.4 μA.cm⁻² for borated 304 L SS), along with a lower pitting potential indicating the role of B. subtilis SNF-1 in MIC. Electrochemical impedance spectroscopy confirmed accelerated degradation, with polarization resistance (R_p) dropping by 69 % in borated 304 L SS and 86 % in non-borated 304 L SS. Despite higher absolute corrosion rates in borated 304 L SS, non-borated 304 L SS experienced a greater relative increase in corrosion (3.8-fold vs. 2.3-fold) due to denser biofilm coverage (95 % vs. 74 %). Surface analysis identified localized pitting beneath biofilms, exacerbated by boride-induced micro-galvanic effects. These findings underscore the dual role of alloy microstructure and biofilm dynamics in MIC severity.

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.