透明质酸三维支架联合高压氧治疗促进糖尿病创面愈合

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-09-09 DOI:10.1016/j.tice.2025.103136

Mohammed Alissa, Suad A. Alghamdi, Abdulkarim S. Binshaya

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引用次数: 0

摘要

糖尿病伤口是一个主要的临床挑战，因为愈合过程受损，如血管生成减少，慢性炎症和胶原蛋白重塑缺陷。本研究旨在评估透明质酸三维支架（HAS）联合高压氧治疗（HBOT）促进糖尿病大鼠模型伤口愈合的治疗潜力。采用Wistar大鼠诱导糖尿病，造全层切除创面。糖尿病动物分为对照组、HBOT组、HAS组和HAS+HBOT组。此外，非糖尿病未经治疗的大鼠（健康组）作为对照。通过伤口收缩率、体视学评估（成纤维细胞计数、中性粒细胞浸润和血管密度）、细胞因子谱（VEGF、TGF-β、TNF-α、IL-1β）、胶原沉积（马松三色、羟脯氨酸测定）和生物力学特性（最大力和能量吸收）来评估伤口愈合进展。与对照组相比，所有治疗组伤口收缩均明显加快，其中HAS+HBOT组改善最大（p <； 0.05）。组织学分析显示，治疗组，特别是HAS+HBOT组，成纤维细胞增殖和新生血管增强，中性粒细胞浸润减少。胶原蛋白含量明显高于处理过的伤口，这与羟脯氨酸水平和三色染色的增加有关。HAS+HBOT组也表现出VEGF和TGF-β水平升高，TNF-α和IL-1β表达降低，表明再生和炎症之间的良好平衡。生物力学测试证实，与所有其他组相比，HAS+HBOT组的最大力和能量吸收都更好。HAS和HBOT联合使用可通过促进组织再生、调节炎症和恢复生物力学完整性来显著促进糖尿病伤口愈合。这一策略有望成为治疗慢性糖尿病伤口的有效方法。

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Hyaluronic acid-based three dimensional scaffold in combination with hyperbaric oxygen therapy promote diabetic wound healing

Diabetic wounds represent a major clinical challenge due to impaired healing processes such as reduced angiogenesis, chronic inflammation, and defective collagen remodeling. This study aimed to evaluate the therapeutic potential of a hyaluronic acid-based three-dimensional scaffold (HAS) in combination with hyperbaric oxygen therapy (HBOT) in promoting wound healing in a diabetic rat model. Diabetes was induced in Wistar rats, and full-thickness excisional wounds were created. Diabetic animals were divided into control, HBOT, HAS, and HAS+HBOT groups. Additionally, non-diabetic untreated rats (Healthy group) were considered as a control. Wound healing progression was assessed via wound contraction rate, stereological evaluations (fibroblast counts, neutrophil infiltration, and blood vessel density), cytokine profiling (VEGF, TGF-β, TNF-α, IL-1β), collagen deposition (Masson's trichrome, hydroxyproline assay), and biomechanical properties (maximum force and energy absorption). Wound contraction was significantly accelerated in all treatment groups compared to controls, with the HAS+HBOT group showing the greatest improvement (p < 0.05). Histological analysis revealed enhanced fibroblast proliferation and neovascularization, along with reduced neutrophil infiltration in treated groups, particularly in the HAS+HBOT group. Collagen content was markedly higher in treated wounds, supported by increased hydroxyproline levels and trichrome staining. The HAS+HBOT group also demonstrated elevated VEGF and TGF-β levels and decreased expression of TNF-α and IL-1β, indicating a favorable balance between regeneration and inflammation. Biomechanical testing confirmed superior maximum force and energy absorption in the HAS+HBOT group compared to all others. The combination of HAS and HBOT significantly enhances diabetic wound healing by promoting tissue regeneration, modulating inflammation, and restoring biomechanical integrity. This strategy holds promise as an effective therapeutic approach for chronic diabetic wounds.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.