超声辅助酸化双水相萃取一步法提取木耳黄酮苷元。花：工艺探索、成分分析和活性验证

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-08-30 DOI:10.1016/j.ultsonch.2025.107545

Tiefeng Yuan , Chen Lin , Qingqing Yu , Xingyu Shi , Peiyi Jin , Jilong Huang , Liping Wang , Huajun Fan

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引用次数: 0

摘要

采用聚乙二醇/硫酸铵体系，建立了一步提取分离木耳黄酮苷元的新方法。采用超声辅助萃取法（UAE）与酸化双水相萃取法（AATPE）相结合的方法制备了花（MACF）。在PEG 600质量分数为22.8% （w/w）、硫酸铵质量分数为22.5% （w/w）、盐酸浓度为1.5 mol/L、水解温度为85℃、反应时间为60 min的最佳UAE-AATPE条件下，MACF类黄酮苷不仅被充分提取，而且被完全水解，所得苷元经双相分离纯化，得率为35.90±0.96 mg/g。通过考察超声场作用下UAE和超声辅助酸水解（UAAH）的不同工艺和化学成分，探讨了UAAH - aatpe工艺的机理。采用高分辨率超高效液相色谱-四极杆轨道阱质谱（UHPLC-Q/ orbitrap - ms）和UHPLC-DAD对去糖基化/糖基化黄酮类化合物进行鉴定和定量，结果表明，MACF黄酮类化合物的含量由高到低依次为天竺葵苷、山酚、儿茶素、花青素和槲皮素。对胰脂肪酶（PL）的抑制实验表明，所提取苷元的IC50为563.01±280.63 μg/mL，其活性强于所提取的苷元。动力学和分子对接分析进一步探索和验证了PL的抑制机制。以上结果证明，UAE-AATPE整合了提取、水解、分离等多道工序，大大简化了复杂的工序，显著提高了提取效率，为直接从MACF中提取黄酮类苷元提供了一种简单、快速、高效的替代方法。

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Ultrasonic-assisted acidified aqueous two-phase extraction for one-step production and recovery of flavonoid aglycones from Malvaviscus arboreus Cav. flower: Process exploration, composition analysis and activity validation

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Using a polyethylene glycol (PEG)/ammonium sulfate system, a novel approach for one-step extraction and separation of the flavonoid aglycones from Malvaviscus arboreus Cav. flower (MACF) was developed by combining ultrasonic-assisted extraction (UAE) with acidified aqueous two-phase extraction (AATPE). Under the optimized UAE-AATPE conditions of PEG 600 of 22.8 % (w/w), ammonium sulfate of 22.5 % (w/w), 1.5 mol/L HCl, hydrolysis temperature of 85 ℃, and reaction time of 60 min, MACF flavonoid glycosides were not only fully extracted but also completely hydrolyzed, and the produced aglycones were purified by biphasic separation with a yield of 35.90 ± 0.96 mg/g. The mechanism in the UAE-AATPE process including UAE and ultrasonic-assisted acid hydrolysis (UAAH), was explored by investigating the different procedures and chemical composition under an ultrasonic field. The deglycosylated/glycosylated flavonoids were identified and quantified by high-resolution ultrahigh-performance liquid chromatography with quadrupole orbitrap mass spectrometry (UHPLC-Q/Orbitrap-MS) and UHPLC-DAD, revealing that MACF flavonoids were mainly composed of pelargonidin, kaempferol, catechin, cyanidin and quercetin in descending order of their content. The inhibitory assay on pancreatic lipase (PL) confirmed that the activity of the extracted aglycones with IC₅₀ of 563.01 ± 280.63 μg/mL was stronger than their glycosides. Kinetic and molecular docking analyses further explored and validated the PL inhibitory mechanism. The above results proved that UAE-AATPE integrating the multiple procedures, including extraction, hydrolysis and separation, greatly simplified the complex procedures and significantly improved extraction efficiency, providing a simple, rapid and efficient alternative for direct obtainment of the flavonoid aglycones from MACF.

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.