中药复方痔疮镇痛膏通过Keap1/Nrf2信号通路促进糖尿病大鼠肛瘘术后创面愈合

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-09-12 DOI:10.1016/j.bbrep.2025.102258

Jian Liu , Zhitao Yin , Xiaojun Tian , Delong Liu , Chunxiao Wang , Hexue Yuan

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引用次数: 0

摘要

本研究旨在阐明痔镇痛药膏（HAC）是如何基于中医“提升与因果”理论，通过调节Keap1/Nrf2信号通路促进糖尿病大鼠肛瘘术后创面愈合的。采用高糖高脂饲料联合链脲佐菌素（STZ）诱导糖尿病大鼠建立肛瘘术后创面模型。将大鼠分为空白对照组、模型组、创面组、实验组（HAC处理）和阳性对照组（重组人表皮生长因子凝胶）。通过糖化血红蛋白（HbA1c）、血清炎症因子（TNF-α、IL-6）、氧化应激标志物（SOD、MDA）和Nrf2通路蛋白和mRNA来评估HAC对伤口愈合的影响。结果显示，HAC组大鼠创面愈合率显著提高（92.85±3.41%,P < 0.01）， HbA1c水平降低（P < 0.05）， TNF-α、IL-6、MDA水平降低（P < 0.01）， SOD活性升高（P < 0.05）， Keap1/Nrf2信号通路被激活。本研究表明，HAC可通过调节Keap1/Nrf2信号通路降低氧化应激和炎症反应，验证其在促进糖尿病（DM）瘘术后创面愈合中的科学价值。

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Chinese traditional medicine complex hemorrhoidal analgesic cream promotes wound healing in diabetic rats after anal fistula surgery via Keap1/Nrf2 signaling pathway

This study to elucidate how hemorrhoidal analgesic cream (HAC), grounded in the traditional Chinese medicine (TCM) ‘elevation and causation’ theory, promotes postoperative wound healing after anal fistula surgery in diabetic rats by modulating the Keap1/Nrf2 signaling pathway. We used high-sugar and high-fat chow combined with streptozotocin (STZ) to induce diabetic rats to establish a postoperative wound model of anal fistula. The rats were divided into a blank control group, model group, wound-only group, experimental group (HAC treatment), and positive control group (recombinant human epidermal growth factor gel). HAC's impact on wound healing was evaluated by glycated hemoglobin (HbA1c), serum inflammatory factors (TNF-α, IL-6), oxidative stress markers (SOD, MDA), and Nrf2 pathway proteins and mRNA. The results showed that the wound healing rate was significantly increased in the HAC group (92.85 ± 3.41 %, P < 0.01), the levels of HbA1c were decreased (P < 0.05), the levels of TNF-α, IL-6, and MDA were decreased (P < 0.01), the activity of SOD was increased (P < 0.05), and the Keap1/Nrf2 signaling pathway was activated. The study demonstrated that HAC could reduce oxidative stress and inflammatory responses by regulating the Keap1/Nrf2 signaling pathway, which validates its scientific value in promoting wound healing after diabetes mellitus (DM) fistula surgery.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.