冬眠前达斡尔地松鼠卵巢颗粒细胞凋亡、自噬和线粒体动力学的上调。

IF 2.2 3区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xi Wang , Hao Li , Zhaomei Dong , Wenjing Lu , Jirui Chen , Haolin Zhang , Zhengrong Yuan , Yuning Liu , Qiang Weng , Yingying Han
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引用次数: 0

摘要

达斡尔地松鼠(spermoophilus dauricus)具有季节性繁殖和冬眠的特征,但在从季节性繁殖到冬眠的过渡过程中,卵巢的适应性变化尚不清楚。本研究旨在探讨野生达斡尔地松鼠卵巢颗粒细胞凋亡、自噬和线粒体动力学在4月(繁殖期)、6月(非繁殖期)和9月(非繁殖期和冬眠前)的调控特征。结果表明,卵巢重量和血清生殖激素在6月和9月较4月显著下降。有趣的是,与6月份不同,野生达斡尔地松鼠在9月份的卵巢中出现了许多正在生长的中腔卵泡。此外,分子研究显示,与6月份相比,9月份卵巢细胞凋亡相关基因(Casp3、Bax/Bcl-2)、自噬(Becn1、Pink1、Parkin、Sqstm1)和线粒体动力学(Mfn1、Mfn2、Drp1)的表达显著增加。此外,免疫组化结果显示上述基因和LC3A/B蛋白水平同时上调,且主要位于卵巢颗粒细胞。与此一致的是,对不同时期卵巢转录组学数据的比较分析进一步证实,在三个时期差异表达的基因在凋亡、自噬和线粒体动力学相关的途径中富集。综上所述,这些结果揭示了冬眠前野生地松鼠卵巢颗粒细胞凋亡、自噬和线粒体动力学的显著上调,可能在冬眠及其后续阶段卵巢发育中起关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Up-regulation of cell apoptosis, autophagy and mitochondrial dynamics in ovarian granulosa cells of Daurian ground squirrels during the pre-hibernation period

Up-regulation of cell apoptosis, autophagy and mitochondrial dynamics in ovarian granulosa cells of Daurian ground squirrels during the pre-hibernation period
Daurian ground squirrel (Spermophilus dauricus) exhibits both seasonal breeding and hibernation characteristics, but the adaptive changes in ovaries during the transition from seasonal reproduction to hibernation remain poorly understood. The aim of this study is to investigate the regulatory characteristics of granulosa cell apoptosis, autophagy and mitochondrial dynamics in the ovaries of wild Daurian ground squirrels across the April (breeding period), June (non-breeding period), and September (non-breeding and pre-hibernation period). The results proved that ovarian weight, as well as serum reproductive hormones, significantly decreased in June and September compared with April. Interestingly, unlike in June, many growing antral follicles emerged in the ovaries of wild Daurian ground squirrels during September. Furthermore, the molecular studies revealed a significant increase in ovarian gene expression related to cell apoptosis (Casp3, Bax/Bcl-2), autophagy (Becn1, Pink1, Parkin, Sqstm1) and mitochondrial dynamics (Mfn1, Mfn2, Drp1) during September compared to June. Additionally, immunohistochemical results indicated that the protein levels of the aforementioned genes and LC3A/B were concurrently upregulated, predominantly located in ovarian granulosa cells. Consistently, comparative analysis of ovarian transcriptomic data across different periods further confirmed that genes differentially expressed during the three periods were enriched in pathways related to apoptosis, autophagy, and mitochondrial dynamics. In summary, these results revealed a significant up-regulation of cell apoptosis, autophagy, and mitochondrial dynamism in ovarian granulosa cells of wild ground squirrels during the pre-hibernation period, may play a critical role in ovarian development for hibernation and subsequent stage.
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来源期刊
CiteScore
5.00
自引率
4.30%
发文量
155
审稿时长
3 months
期刊介绍: Part A: Molecular & Integrative Physiology of Comparative Biochemistry and Physiology. This journal covers molecular, cellular, integrative, and ecological physiology. Topics include bioenergetics, circulation, development, excretion, ion regulation, endocrinology, neurobiology, nutrition, respiration, and thermal biology. Study on regulatory mechanisms at any level of organization such as signal transduction and cellular interaction and control of behavior are also published.
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