Xi Wang , Hao Li , Zhaomei Dong , Wenjing Lu , Jirui Chen , Haolin Zhang , Zhengrong Yuan , Yuning Liu , Qiang Weng , Yingying Han
{"title":"冬眠前达斡尔地松鼠卵巢颗粒细胞凋亡、自噬和线粒体动力学的上调。","authors":"Xi Wang , Hao Li , Zhaomei Dong , Wenjing Lu , Jirui Chen , Haolin Zhang , Zhengrong Yuan , Yuning Liu , Qiang Weng , Yingying Han","doi":"10.1016/j.cbpa.2025.111927","DOIUrl":null,"url":null,"abstract":"<div><div>Daurian ground squirrel (<em>Spermophilus dauricus</em>) exhibits both seasonal breeding and hibernation characteristics, but the adaptive changes in ovaries during the transition from seasonal reproduction to hibernation remain poorly understood. The aim of this study is to investigate the regulatory characteristics of granulosa cell apoptosis, autophagy and mitochondrial dynamics in the ovaries of wild Daurian ground squirrels across the April (breeding period), June (non-breeding period), and September (non-breeding and pre-hibernation period). The results proved that ovarian weight, as well as serum reproductive hormones, significantly decreased in June and September compared with April. Interestingly, unlike in June, many growing antral follicles emerged in the ovaries of wild Daurian ground squirrels during September. Furthermore, the molecular studies revealed a significant increase in ovarian gene expression related to cell apoptosis (<em>Casp3, Bax/Bcl-2</em>), autophagy (<em>Becn1, Pink1, Parkin, Sqstm1</em>) and mitochondrial dynamics (<em>Mfn1, Mfn2, Drp1</em>) during September compared to June. Additionally, immunohistochemical results indicated that the protein levels of the aforementioned genes and LC3A/B were concurrently upregulated, predominantly located in ovarian granulosa cells. Consistently, comparative analysis of ovarian transcriptomic data across different periods further confirmed that genes differentially expressed during the three periods were enriched in pathways related to apoptosis, autophagy, and mitochondrial dynamics. In summary, these results revealed a significant up-regulation of cell apoptosis, autophagy, and mitochondrial dynamism in ovarian granulosa cells of wild ground squirrels during the pre-hibernation period, may play a critical role in ovarian development for hibernation and subsequent stage.</div></div>","PeriodicalId":55237,"journal":{"name":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","volume":"310 ","pages":"Article 111927"},"PeriodicalIF":2.2000,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Up-regulation of cell apoptosis, autophagy and mitochondrial dynamics in ovarian granulosa cells of Daurian ground squirrels during the pre-hibernation period\",\"authors\":\"Xi Wang , Hao Li , Zhaomei Dong , Wenjing Lu , Jirui Chen , Haolin Zhang , Zhengrong Yuan , Yuning Liu , Qiang Weng , Yingying Han\",\"doi\":\"10.1016/j.cbpa.2025.111927\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Daurian ground squirrel (<em>Spermophilus dauricus</em>) exhibits both seasonal breeding and hibernation characteristics, but the adaptive changes in ovaries during the transition from seasonal reproduction to hibernation remain poorly understood. The aim of this study is to investigate the regulatory characteristics of granulosa cell apoptosis, autophagy and mitochondrial dynamics in the ovaries of wild Daurian ground squirrels across the April (breeding period), June (non-breeding period), and September (non-breeding and pre-hibernation period). The results proved that ovarian weight, as well as serum reproductive hormones, significantly decreased in June and September compared with April. Interestingly, unlike in June, many growing antral follicles emerged in the ovaries of wild Daurian ground squirrels during September. Furthermore, the molecular studies revealed a significant increase in ovarian gene expression related to cell apoptosis (<em>Casp3, Bax/Bcl-2</em>), autophagy (<em>Becn1, Pink1, Parkin, Sqstm1</em>) and mitochondrial dynamics (<em>Mfn1, Mfn2, Drp1</em>) during September compared to June. Additionally, immunohistochemical results indicated that the protein levels of the aforementioned genes and LC3A/B were concurrently upregulated, predominantly located in ovarian granulosa cells. Consistently, comparative analysis of ovarian transcriptomic data across different periods further confirmed that genes differentially expressed during the three periods were enriched in pathways related to apoptosis, autophagy, and mitochondrial dynamics. In summary, these results revealed a significant up-regulation of cell apoptosis, autophagy, and mitochondrial dynamism in ovarian granulosa cells of wild ground squirrels during the pre-hibernation period, may play a critical role in ovarian development for hibernation and subsequent stage.</div></div>\",\"PeriodicalId\":55237,\"journal\":{\"name\":\"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology\",\"volume\":\"310 \",\"pages\":\"Article 111927\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-09-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1095643325001266\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1095643325001266","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Up-regulation of cell apoptosis, autophagy and mitochondrial dynamics in ovarian granulosa cells of Daurian ground squirrels during the pre-hibernation period
Daurian ground squirrel (Spermophilus dauricus) exhibits both seasonal breeding and hibernation characteristics, but the adaptive changes in ovaries during the transition from seasonal reproduction to hibernation remain poorly understood. The aim of this study is to investigate the regulatory characteristics of granulosa cell apoptosis, autophagy and mitochondrial dynamics in the ovaries of wild Daurian ground squirrels across the April (breeding period), June (non-breeding period), and September (non-breeding and pre-hibernation period). The results proved that ovarian weight, as well as serum reproductive hormones, significantly decreased in June and September compared with April. Interestingly, unlike in June, many growing antral follicles emerged in the ovaries of wild Daurian ground squirrels during September. Furthermore, the molecular studies revealed a significant increase in ovarian gene expression related to cell apoptosis (Casp3, Bax/Bcl-2), autophagy (Becn1, Pink1, Parkin, Sqstm1) and mitochondrial dynamics (Mfn1, Mfn2, Drp1) during September compared to June. Additionally, immunohistochemical results indicated that the protein levels of the aforementioned genes and LC3A/B were concurrently upregulated, predominantly located in ovarian granulosa cells. Consistently, comparative analysis of ovarian transcriptomic data across different periods further confirmed that genes differentially expressed during the three periods were enriched in pathways related to apoptosis, autophagy, and mitochondrial dynamics. In summary, these results revealed a significant up-regulation of cell apoptosis, autophagy, and mitochondrial dynamism in ovarian granulosa cells of wild ground squirrels during the pre-hibernation period, may play a critical role in ovarian development for hibernation and subsequent stage.
期刊介绍:
Part A: Molecular & Integrative Physiology of Comparative Biochemistry and Physiology. This journal covers molecular, cellular, integrative, and ecological physiology. Topics include bioenergetics, circulation, development, excretion, ion regulation, endocrinology, neurobiology, nutrition, respiration, and thermal biology. Study on regulatory mechanisms at any level of organization such as signal transduction and cellular interaction and control of behavior are also published.