单次口服曲唑酮在家兔体内的药代动力学和行为效应。

IF 1.9 2区 农林科学 Q2 VETERINARY SCIENCES
Adalaïs Gibert, Ryota Watanabe, Marta Garbin, Tristan Juette, Francis Beaudry, Marion Desmarchelier, Inga-Catalina Cruz Benedetti
{"title":"单次口服曲唑酮在家兔体内的药代动力学和行为效应。","authors":"Adalaïs Gibert, Ryota Watanabe, Marta Garbin, Tristan Juette, Francis Beaudry, Marion Desmarchelier, Inga-Catalina Cruz Benedetti","doi":"10.1016/j.vaa.2025.06.006","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the pharmacokinetics and pharmacodynamics of a single oral dose of trazodone in laboratory rabbits.</p><p><strong>Study design: </strong>Randomised, blinded, Latin-square study.</p><p><strong>Animals: </strong>A group of eight healthy, juvenile, female New Zealand White rabbits.</p><p><strong>Methods: </strong>Auricular venous catheters were placed in six rabbits before oral trazodone (20 mg kg<sup>-1</sup>) administration. Blood was sampled immediately before and at 15 and 30 minutes and 1, 2, 4, 8, 12, 18 and 24 hours after treatment. Trazodone plasma concentrations were determined using high-performance liquid chromatography-mass spectrometry. A group of eight rabbits were equipped with accelerometers (activity) and video recorded. They randomly received one of three treatments: oral trazodone (20 mg kg<sup>-1</sup>; TRAZ), placebo (corn oil base; PLAC) or no treatment (CONTR) with a 3 day washout period between treatments. Exploring, grooming, resting, vigilance, hiding and ingesting behaviours were evaluated for 10 hours (0-2, 2-4, 4-6, 6-8 and 8-10 hours).</p><p><strong>Results: </strong>Mean maximum plasma concentration, 6592.6 ± 586.5 ng mL<sup>-1</sup>, was 0.3 ± 0.11 hours after treatment. Mean half-life was 3.24 ± 0.36 hours and area under the curve from zero to infinity 25,412.5 ± 5420.9 ng mL<sup>-1</sup> hour<sup>-1</sup>. Activity was higher in TRAZ and PLAC than CONTR at 0-2 hours, with TRAZ exploring more. Hiding was reduced in TRAZ versus CONTR (0-2 hours and 4-6 hours) and versus PLAC (0-6 hours). Resting increased in TRAZ versus PLAC (2-4 hours) and CONTR (4-6 hours); PLAC rested less at 2-4 hours than at 8-10 hours. Vigilance tended to be lower in TRAZ versus CONTR. Food intake decreased in TRAZ at 2-4 hours. Grooming varied over time.</p><p><strong>Conclusions and clinical relevance: </strong>Trazodone-treated animals showed reduced activity, less hiding and more resting than PLAC and decreased food intake 2-4 hours post-treatment.</p>","PeriodicalId":23626,"journal":{"name":"Veterinary anaesthesia and analgesia","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pharmacokinetics and behavioural effects of a single oral dose of trazodone in rabbits.\",\"authors\":\"Adalaïs Gibert, Ryota Watanabe, Marta Garbin, Tristan Juette, Francis Beaudry, Marion Desmarchelier, Inga-Catalina Cruz Benedetti\",\"doi\":\"10.1016/j.vaa.2025.06.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To evaluate the pharmacokinetics and pharmacodynamics of a single oral dose of trazodone in laboratory rabbits.</p><p><strong>Study design: </strong>Randomised, blinded, Latin-square study.</p><p><strong>Animals: </strong>A group of eight healthy, juvenile, female New Zealand White rabbits.</p><p><strong>Methods: </strong>Auricular venous catheters were placed in six rabbits before oral trazodone (20 mg kg<sup>-1</sup>) administration. Blood was sampled immediately before and at 15 and 30 minutes and 1, 2, 4, 8, 12, 18 and 24 hours after treatment. Trazodone plasma concentrations were determined using high-performance liquid chromatography-mass spectrometry. A group of eight rabbits were equipped with accelerometers (activity) and video recorded. They randomly received one of three treatments: oral trazodone (20 mg kg<sup>-1</sup>; TRAZ), placebo (corn oil base; PLAC) or no treatment (CONTR) with a 3 day washout period between treatments. Exploring, grooming, resting, vigilance, hiding and ingesting behaviours were evaluated for 10 hours (0-2, 2-4, 4-6, 6-8 and 8-10 hours).</p><p><strong>Results: </strong>Mean maximum plasma concentration, 6592.6 ± 586.5 ng mL<sup>-1</sup>, was 0.3 ± 0.11 hours after treatment. Mean half-life was 3.24 ± 0.36 hours and area under the curve from zero to infinity 25,412.5 ± 5420.9 ng mL<sup>-1</sup> hour<sup>-1</sup>. Activity was higher in TRAZ and PLAC than CONTR at 0-2 hours, with TRAZ exploring more. Hiding was reduced in TRAZ versus CONTR (0-2 hours and 4-6 hours) and versus PLAC (0-6 hours). Resting increased in TRAZ versus PLAC (2-4 hours) and CONTR (4-6 hours); PLAC rested less at 2-4 hours than at 8-10 hours. Vigilance tended to be lower in TRAZ versus CONTR. Food intake decreased in TRAZ at 2-4 hours. Grooming varied over time.</p><p><strong>Conclusions and clinical relevance: </strong>Trazodone-treated animals showed reduced activity, less hiding and more resting than PLAC and decreased food intake 2-4 hours post-treatment.</p>\",\"PeriodicalId\":23626,\"journal\":{\"name\":\"Veterinary anaesthesia and analgesia\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2025-06-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary anaesthesia and analgesia\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.vaa.2025.06.006\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary anaesthesia and analgesia","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.vaa.2025.06.006","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

目的:评价单次口服曲唑酮在家兔体内的药动学和药效学。研究设计:随机、盲法、拉丁方研究。动物:一群8只健康的幼年雌性新西兰白兔。方法:6只家兔在口服曲唑酮(20mg kg-1)前放置耳静脉导管。在治疗前、15分钟和30分钟以及治疗后1、2、4、8、12、18和24小时采集血样。采用高效液相色谱-质谱法测定曲唑酮血药浓度。一组8只兔子配备了加速度计(活动)并记录了视频。他们随机接受三种治疗中的一种:口服曲唑酮(20 mg kg-1; TRAZ),安慰剂(玉米油基;placc)或不治疗(CONTR),治疗之间有3天的洗脱期。10小时(0-2、2-4、4-6、6-8和8-10小时)评估探索、梳理、休息、警戒、躲藏和摄食行为。结果:治疗后0.3±0.11 h血药浓度平均最高为6592.6±586.5 ng mL-1。平均半衰期为3.24±0.36小时,曲线下面积为25,412.5±5420.9 ng mL-1 hour-1。在0-2小时,TRAZ和placc的活性高于contrr, TRAZ探索更多。与对照组(0-2小时和4-6小时)和对照组(0-6小时)相比,TRAZ组的隐藏程度有所降低。与plac2 -4小时和contr4 -6小时相比,TRAZ的静息时间增加;2-4小时的休息时间少于8-10小时。与对照组相比,TRAZ的警惕性往往较低。在2-4小时内,TRAZ的食物摄入量减少。打扮随着时间的推移而变化。结论及临床意义:治疗后2-4小时,曲唑酮治疗的动物活性降低,躲藏更少,休息时间更长,食物摄入量减少。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pharmacokinetics and behavioural effects of a single oral dose of trazodone in rabbits.

Objective: To evaluate the pharmacokinetics and pharmacodynamics of a single oral dose of trazodone in laboratory rabbits.

Study design: Randomised, blinded, Latin-square study.

Animals: A group of eight healthy, juvenile, female New Zealand White rabbits.

Methods: Auricular venous catheters were placed in six rabbits before oral trazodone (20 mg kg-1) administration. Blood was sampled immediately before and at 15 and 30 minutes and 1, 2, 4, 8, 12, 18 and 24 hours after treatment. Trazodone plasma concentrations were determined using high-performance liquid chromatography-mass spectrometry. A group of eight rabbits were equipped with accelerometers (activity) and video recorded. They randomly received one of three treatments: oral trazodone (20 mg kg-1; TRAZ), placebo (corn oil base; PLAC) or no treatment (CONTR) with a 3 day washout period between treatments. Exploring, grooming, resting, vigilance, hiding and ingesting behaviours were evaluated for 10 hours (0-2, 2-4, 4-6, 6-8 and 8-10 hours).

Results: Mean maximum plasma concentration, 6592.6 ± 586.5 ng mL-1, was 0.3 ± 0.11 hours after treatment. Mean half-life was 3.24 ± 0.36 hours and area under the curve from zero to infinity 25,412.5 ± 5420.9 ng mL-1 hour-1. Activity was higher in TRAZ and PLAC than CONTR at 0-2 hours, with TRAZ exploring more. Hiding was reduced in TRAZ versus CONTR (0-2 hours and 4-6 hours) and versus PLAC (0-6 hours). Resting increased in TRAZ versus PLAC (2-4 hours) and CONTR (4-6 hours); PLAC rested less at 2-4 hours than at 8-10 hours. Vigilance tended to be lower in TRAZ versus CONTR. Food intake decreased in TRAZ at 2-4 hours. Grooming varied over time.

Conclusions and clinical relevance: Trazodone-treated animals showed reduced activity, less hiding and more resting than PLAC and decreased food intake 2-4 hours post-treatment.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Veterinary anaesthesia and analgesia
Veterinary anaesthesia and analgesia 农林科学-兽医学
CiteScore
3.10
自引率
17.60%
发文量
91
审稿时长
97 days
期刊介绍: Veterinary Anaesthesia and Analgesia is the official journal of the Association of Veterinary Anaesthetists, the American College of Veterinary Anesthesia and Analgesia and the European College of Veterinary Anaesthesia and Analgesia. Its purpose is the publication of original, peer reviewed articles covering all branches of anaesthesia and the relief of pain in animals. Articles concerned with the following subjects related to anaesthesia and analgesia are also welcome: the basic sciences; pathophysiology of disease as it relates to anaesthetic management equipment intensive care chemical restraint of animals including laboratory animals, wildlife and exotic animals welfare issues associated with pain and distress education in veterinary anaesthesia and analgesia. Review articles, special articles, and historical notes will also be published, along with editorials, case reports in the form of letters to the editor, and book reviews. There is also an active correspondence section.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信