Jing Chen, Rong Zhang, Xun Zhang, Chengjin Li, Jie Yang, Xiangbo Kong, Fu Liu, Jiaxing Fang, Sufang Zhang
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Sustainable protection of multiple hosts against polyphagous pests using Plant Probiotic-Based Gene Silencing.
RNAi is a gene-silencing mechanism mediated by double-stranded RNA (dsRNA) molecules and is a promising pest control technology. However, challenges, such as limited persistence or narrow plant protection spectra in current RNAi application strategies, hinder its effectiveness against polyphagous pests. Here, we report an approach using plant probiotics to express dsRNA, propelling sustainable protection of multiple host plants. A Bacillus strain isolated from host plants of the polyphagous pest Hyphantria cunea, was engineered to express dsRNA targeting the pest. The modified SH-F8 strain significantly reduced H. cunea pupation rates and increased mortality by disrupting both energy metabolism and cuticle formation. The engineered SH-F8 strain exhibited successful colonization in two host plant species of H. cunea under field conditions, with enhanced population densities appearing under high-temperature/high-humidity conditions. This approach, termed 'Plant Probiotic-Based Gene Silencing' (PPGS), may offer a sustainable solution for multi-plant protection against polyphagous pests.
期刊介绍：
Trends in Biotechnology publishes reviews and perspectives on the applied biological sciences, focusing on useful science applied to, derived from, or inspired by living systems.
The major themes that TIBTECH is interested in include:
Bioprocessing (biochemical engineering, applied enzymology, industrial biotechnology, biofuels, metabolic engineering)
Omics (genome editing, single-cell technologies, bioinformatics, synthetic biology)
Materials and devices (bionanotechnology, biomaterials, diagnostics/imaging/detection, soft robotics, biosensors/bioelectronics)
Therapeutics (biofabrication, stem cells, tissue engineering and regenerative medicine, antibodies and other protein drugs, drug delivery)
Agroenvironment (environmental engineering, bioremediation, genetically modified crops, sustainable development).
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