Shuai Hou, Hong Zhou, Jinxiong Lv, Peng Chen, Caixia Li, Yu Lin, Yueyue Liu, Yaxi Liu
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Fine-mapping the low number of tillers (lnt2) locus in barley.
Tiller number is an essential agronomic characteristic that influences barley morphology and yield. A barley low number of tillers mutant CIHO 11,530 exhibits few tillers and in this study, we conducted a genetic analysis of the barley low number of tillers 2 (lnt2) locus. Linkage analysis showed that lnt2 was mapped in an interval of 3.39 cM on chromosome 6HS between the flanking markers SNP1765 and SNP526, explaining 53.06% of the phenotypic variance. The genetic effect of lnt2 was further verified in two other genetic backgrounds, explaining variances of 86.43% and 91.01% in tiller numbers between lines carrying the lnt2 mutant and wild-type alleles, respectively. Furthermore, we constructed a large F2 population and fine-mapped lnt2. Finally, lnt2 was mapped within a 0.19 cM genetic interval delimited by the tightly linked KASP markers KASP6359 and KASP365, and the physical interval was located at 40.57-42.35 Mb. In this interval, three genes were highly likely lnt2 based on gene annotations, sequence and gene expression analyses. Our research provides valuable information for the map-based cloning of lnt2.
Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01589-7.
期刊介绍：
Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer.
All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others.
Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards.
Molecular Breeding core areas:
Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.
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