Rhizophagus irregularis DAOM197198在丛枝菌根共生关系的建立和功能中调控短根紫花苜蓿根泛素。

IF 3.8 2区 生物学 Q2 MYCOLOGY
Damien Inès, Carole Pichereaux, David Wendehenne, Pierre Emmanuel Courty, Claire Rosnoblet
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引用次数: 0

摘要

细胞蛋白稳态的调节涉及到泛素-蛋白酶体系统(UPS)通过选择性靶向错误折叠或生命终止蛋白。生物胁迫中UPS的研究主要集中在植物与病原体的相互作用上,而在植物与共生的相互作用上研究较少。本研究通过蛋白质组学方法(western blot、多泛素化蛋白下拉和纳米lc - ms - ms分析)研究了UPS在真菌Rhizophagus irregularis DAOM197198与Medicago truncatula根系之间建立互利相互作用过程中的作用,以及在建立共生关系中的作用。接种后0 h、3 h、6 h、9 h、12 h、24 h和15 d分别收获根。我们表征了根泛素组的短时间和长时间响应。一些蛋白质如flotilins或参与翻译机制的蛋白质泛素化程度较低,这表明建立丛枝菌根共生所需的蛋白质的重新合成更容易。相比之下,其他通过直接途径参与植物营养的转运蛋白(如MtPT5)和一些参与脂质生物合成途径的酶则更泛素化,这表明它们可能会被降解。此外,Cdc48蛋白在接种后9至24 h在根中积累,表明Cdc48在植物与真菌相互作用期间的短暂免疫应答中起作用。因此,通过调节蛋白质泛素化,UPS的活性在丛枝菌根共生的建立和功能中起着中心作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rhizophagus irregularis DAOM197198 modulates the root ubiquitinome of Medicago truncatula in the establishment and functioning of arbuscular mycorrhizal symbiosis.

The regulation of cellular protein homeostasis involves the ubiquitin-proteasome system (UPS) by selectively targeting misfolded or end-of-life proteins. The involvement of the UPS in biotic stresses has been studied mainly in plant-pathogen interactions and poorly in plant-mutualistic interactions. Here, we studied through proteomic approaches (western blot, pull-down of polyubiquinated proteins and nano-LC-MS-MS analysis), the involvement of the UPS during the establishment of the mutualistic interaction between the arbuscular mycorrhizal fungus Rhizophagus irregularis DAOM197198 and the roots of Medicago truncatula, as well as in the established symbiosis. Roots of M. truncatula seedlings were harvested 0 h, 3 h, 6 h, 9 h, 12 h, 24 h and 15 days post-inoculation. We characterized a short-time and a-long-time response of the root ubiquitinome. Some proteins as such as flotilins or involved in the translational machinery were less-ubiquitinated, suggesting the facilitation of the de novo synthesis of proteins required to the establishment of arbuscular mycorrhizal symbiosis. In contrast, other proteins as transporters involved in plant nutrition through the direct pathway (i.e., MtPT5) and some enzymes involved in the lipid biosynthesis pathways were more-ubiquitinated, highlighting their putative degradation. In addition, Cdc48 protein accumulates in roots from 9 to 24 h post-inoculation, suggesting a role of Cdc48 in the transitory immune response during plant-fungal interactions. The activity of the UPS is consequently central in the establishment and functioning of arbuscular mycorrhizal symbiosis by modulating protein ubiquitination.

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来源期刊
Mycorrhiza
Mycorrhiza 生物-真菌学
CiteScore
8.20
自引率
2.60%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The scope of Mycorrhiza covers all aspects of research into mycorrhizas, including molecular biology of the plants and fungi, fungal systematics, development and structure of mycorrhizas, and effects on plant physiology, productivity, reproduction and disease resistance. The scope also includes interactions between mycorrhizal fungi and other soil organisms and effects of mycorrhizas on plant biodiversity and ecosystem structure. Mycorrhiza contains original papers, short notes and review articles, along with commentaries and news items. It forms a platform for new concepts and discussions, and is a basis for a truly international forum of mycorrhizologists from all over the world.
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