Sung-Eun Cho, Jungsun Han, Gayoung Chun, Rihwa Choi, Sang Gon Lee, Hyung-Doo Park
{"title":"用UPLC-MS/MS评价韩国人群血清3- epi -25-羟维生素D3、25-羟维生素D3和25-羟维生素D2","authors":"Sung-Eun Cho, Jungsun Han, Gayoung Chun, Rihwa Choi, Sang Gon Lee, Hyung-Doo Park","doi":"10.1002/jcla.70098","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>The liquid chromatography–tandem mass spectrometry method (LC–MS/MS) identified an epimeric form of 25-hydroxyvitamin D<sub>3</sub> (3-epi-25-OH-D<sub>3</sub>) concentrations. For the first time, the 3-epi-25-OH-D<sub>3</sub> was included in the Korea National Health and Nutrition Examination Survey (KNHANES) IX (from 2022 to 2024). In this study, we evaluated the results of 3-epi-25-OH-D<sub>3</sub> in KNHANES IX to determine its clinical status in Korea.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>We measured 3-epi-OH-D<sub>3</sub>, 25-OH-D<sub>3</sub>, and 25-OH-D<sub>2</sub> concentrations using an Ultra Performance (UP) LC–MS/MS, and assessed the differences in concentration ranges based on gender and age among participants in this national project.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The concentration range of 3-epi-25-OH-D<sub>3</sub> among 11,744 Korean participants (5193 male and 6551 female, aged 10–80 with a median age of 53) was 0.50 (lower limit of quantification) to 15.66 ng/mL, with a mean ± SD of 1.15 ± 0.85 ng/mL. The 3-epi-25-OH-D<sub>3</sub> concentration was not significantly different between genders. The concentrations of 25-OH-D metabolites were different across age groups. There was a highly significant correlation between 3-epi-25-OH-D<sub>3</sub> and 25-OH-D<sub>3</sub> concentrations (<i>r</i> = 0.77, <i>R</i><sup>2</sup> = 0.5911, slope = 0.0579, <i>p</i> < 0.0001). The minimum and maximum of % 3-epi-25-OH-D<sub>3</sub> were 1.33% and 23.94%, respectively. The % 3-epi-25-OH-D<sub>3</sub> was highest in the highest quartile (Q4) of the total 25-OH-D<sub>3</sub> concentration (<i>p</i> < 0.0001).</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>This is the first large-scale study of 3-epi-25-OH-D<sub>3</sub> concentrations in the Korean population. Accurate measurement of 3-epi-25-OH-D<sub>3</sub> is necessary for the reliable results of vitamin D metabolites in KNHANES.</p>\n </section>\n </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"39 19","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70098","citationCount":"0","resultStr":"{\"title\":\"Assessment of Serum 3-Epi-25-Hydroxyvitamin D3, 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 in the Korean Population With UPLC–MS/MS\",\"authors\":\"Sung-Eun Cho, Jungsun Han, Gayoung Chun, Rihwa Choi, Sang Gon Lee, Hyung-Doo Park\",\"doi\":\"10.1002/jcla.70098\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>The liquid chromatography–tandem mass spectrometry method (LC–MS/MS) identified an epimeric form of 25-hydroxyvitamin D<sub>3</sub> (3-epi-25-OH-D<sub>3</sub>) concentrations. For the first time, the 3-epi-25-OH-D<sub>3</sub> was included in the Korea National Health and Nutrition Examination Survey (KNHANES) IX (from 2022 to 2024). In this study, we evaluated the results of 3-epi-25-OH-D<sub>3</sub> in KNHANES IX to determine its clinical status in Korea.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>We measured 3-epi-OH-D<sub>3</sub>, 25-OH-D<sub>3</sub>, and 25-OH-D<sub>2</sub> concentrations using an Ultra Performance (UP) LC–MS/MS, and assessed the differences in concentration ranges based on gender and age among participants in this national project.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The concentration range of 3-epi-25-OH-D<sub>3</sub> among 11,744 Korean participants (5193 male and 6551 female, aged 10–80 with a median age of 53) was 0.50 (lower limit of quantification) to 15.66 ng/mL, with a mean ± SD of 1.15 ± 0.85 ng/mL. The 3-epi-25-OH-D<sub>3</sub> concentration was not significantly different between genders. The concentrations of 25-OH-D metabolites were different across age groups. There was a highly significant correlation between 3-epi-25-OH-D<sub>3</sub> and 25-OH-D<sub>3</sub> concentrations (<i>r</i> = 0.77, <i>R</i><sup>2</sup> = 0.5911, slope = 0.0579, <i>p</i> < 0.0001). The minimum and maximum of % 3-epi-25-OH-D<sub>3</sub> were 1.33% and 23.94%, respectively. The % 3-epi-25-OH-D<sub>3</sub> was highest in the highest quartile (Q4) of the total 25-OH-D<sub>3</sub> concentration (<i>p</i> < 0.0001).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>This is the first large-scale study of 3-epi-25-OH-D<sub>3</sub> concentrations in the Korean population. Accurate measurement of 3-epi-25-OH-D<sub>3</sub> is necessary for the reliable results of vitamin D metabolites in KNHANES.</p>\\n </section>\\n </div>\",\"PeriodicalId\":15509,\"journal\":{\"name\":\"Journal of Clinical Laboratory Analysis\",\"volume\":\"39 19\",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcla.70098\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Clinical Laboratory Analysis\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jcla.70098\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Laboratory Analysis","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jcla.70098","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
Assessment of Serum 3-Epi-25-Hydroxyvitamin D3, 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 in the Korean Population With UPLC–MS/MS
Background
The liquid chromatography–tandem mass spectrometry method (LC–MS/MS) identified an epimeric form of 25-hydroxyvitamin D3 (3-epi-25-OH-D3) concentrations. For the first time, the 3-epi-25-OH-D3 was included in the Korea National Health and Nutrition Examination Survey (KNHANES) IX (from 2022 to 2024). In this study, we evaluated the results of 3-epi-25-OH-D3 in KNHANES IX to determine its clinical status in Korea.
Methods
We measured 3-epi-OH-D3, 25-OH-D3, and 25-OH-D2 concentrations using an Ultra Performance (UP) LC–MS/MS, and assessed the differences in concentration ranges based on gender and age among participants in this national project.
Results
The concentration range of 3-epi-25-OH-D3 among 11,744 Korean participants (5193 male and 6551 female, aged 10–80 with a median age of 53) was 0.50 (lower limit of quantification) to 15.66 ng/mL, with a mean ± SD of 1.15 ± 0.85 ng/mL. The 3-epi-25-OH-D3 concentration was not significantly different between genders. The concentrations of 25-OH-D metabolites were different across age groups. There was a highly significant correlation between 3-epi-25-OH-D3 and 25-OH-D3 concentrations (r = 0.77, R2 = 0.5911, slope = 0.0579, p < 0.0001). The minimum and maximum of % 3-epi-25-OH-D3 were 1.33% and 23.94%, respectively. The % 3-epi-25-OH-D3 was highest in the highest quartile (Q4) of the total 25-OH-D3 concentration (p < 0.0001).
Conclusions
This is the first large-scale study of 3-epi-25-OH-D3 concentrations in the Korean population. Accurate measurement of 3-epi-25-OH-D3 is necessary for the reliable results of vitamin D metabolites in KNHANES.
期刊介绍：
Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.
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