{"title":"肺泡ii型样细胞集落:肺泡巨噬细胞和巨噬细胞条件培养基的作用","authors":"Moshe Kalina, Shoshana Riklis","doi":"10.1016/0045-6039(88)90076-0","DOIUrl":null,"url":null,"abstract":"<div><p>Alveolar type II-like colonies were obtained after a low density plating (5 × 10<sup>3</sup>/60 mm tissue culture dish) of primary type II cells. These colonies were formed only when type II cells were either cocultured with alveolar macrophages or with conditioned media generated by alveolar macrophages. Cells in the colonies appeared homogeneous and kept their lamellar bodies over a period of 8 weeks and more, as observed by electron microscopy. These cells reacted immunocytochemically with antibodies directed against the 32–38 kDa protein fractions of rat surfactant.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 3","pages":"Pages 231-236"},"PeriodicalIF":0.0000,"publicationDate":"1988-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90076-0","citationCount":"12","resultStr":"{\"title\":\"Alveolar type II-like cell colonies: effect of alveolar macrophages and macrophage-conditioned media\",\"authors\":\"Moshe Kalina, Shoshana Riklis\",\"doi\":\"10.1016/0045-6039(88)90076-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Alveolar type II-like colonies were obtained after a low density plating (5 × 10<sup>3</sup>/60 mm tissue culture dish) of primary type II cells. These colonies were formed only when type II cells were either cocultured with alveolar macrophages or with conditioned media generated by alveolar macrophages. Cells in the colonies appeared homogeneous and kept their lamellar bodies over a period of 8 weeks and more, as observed by electron microscopy. These cells reacted immunocytochemically with antibodies directed against the 32–38 kDa protein fractions of rat surfactant.</p></div>\",\"PeriodicalId\":75684,\"journal\":{\"name\":\"Cell differentiation\",\"volume\":\"23 3\",\"pages\":\"Pages 231-236\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0045-6039(88)90076-0\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cell differentiation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0045603988900760\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell differentiation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0045603988900760","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Alveolar type II-like cell colonies: effect of alveolar macrophages and macrophage-conditioned media
Alveolar type II-like colonies were obtained after a low density plating (5 × 103/60 mm tissue culture dish) of primary type II cells. These colonies were formed only when type II cells were either cocultured with alveolar macrophages or with conditioned media generated by alveolar macrophages. Cells in the colonies appeared homogeneous and kept their lamellar bodies over a period of 8 weeks and more, as observed by electron microscopy. These cells reacted immunocytochemically with antibodies directed against the 32–38 kDa protein fractions of rat surfactant.
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