抑郁症的环境和遗传风险因素集中在由胆固醇稳态变化驱动的神经元功能障碍上。

IF 8.7 1区 生物学 Q1 CELL BIOLOGY
Polina Oberst, Nan Xu, Hermany Munguba, Chao Zhang, Aaron Zhong, Ting Zhou, Conor Liston, Joshua Levitz, Lorenz Studer
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引用次数: 0

摘要

重度抑郁症(MDD)是一种复杂的、多因素的神经精神疾病,受遗传和环境因素的影响,但这些因素如何影响人类神经元功能尚不清楚。使用高度定义的人类多能干细胞(hPSC)为基础的前额叶皮质(PFC)平台,我们研究了与抑郁症相关的三个高可信度的环境和遗传因素:长期暴露于高水平的皮质醇或干扰素α (IFN-a),以及SIRTUIN 1 (SIRT1)的突变。这三种情况都会导致神经元功能障碍的重叠表型,其特征是树突萎缩、突触丧失和多个细胞系的神经元活性降低。RNA测序揭示了神经元胆固醇稳态的趋同改变。在对照神经元中消耗胆固醇可复制核心抑郁相关神经元表型,而在抑郁相关条件下补充胆固醇足以挽救这些表型。这些发现表明,胆固醇失衡是重度抑郁症神经元功能障碍的共同驱动因素,通过共享的细胞途径将多种遗传和环境风险因素联系起来。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Environmental and genetic risk factors of depression converge on neuronal dysfunction driven by changes in cholesterol homeostasis.

Environmental and genetic risk factors of depression converge on neuronal dysfunction driven by changes in cholesterol homeostasis.

Major depressive disorder (MDD) is a complex, multifactorial neuropsychiatric disorder influenced by both genetic and environmental factors, but how these factors impact human neuronal function remains unclear. Using a highly defined human pluripotent stem cell (hPSC)-based prefrontal cortex (PFC) platform, we examined three high-confidence environmental and genetic factors associated with depression: chronic exposure to high levels of cortisol or interferon alpha (IFN-a), and a mutation in SIRTUIN 1 (SIRT1). All three conditions induced overlapping phenotypes of neuronal dysfunction, characterized by dendritic atrophy, synaptic loss, and neuronal hypoactivity across multiple cell lines. RNA sequencing uncovered converging alterations in neuronal cholesterol homeostasis. Depleting cholesterol in control neurons reproduced core depression-associated neuronal phenotypes, while cholesterol supplementation was sufficient to rescue these phenotypes in depression-associated conditions. These findings point to cholesterol imbalance as a common driver of neuronal dysfunction in MDD, linking diverse genetic and environmental risk factors through a shared cellular pathway.

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来源期刊
Developmental cell
Developmental cell 生物-发育生物学
CiteScore
18.90
自引率
1.70%
发文量
203
审稿时长
3-6 weeks
期刊介绍: Developmental Cell, established in 2001, is a comprehensive journal that explores a wide range of topics in cell and developmental biology. Our publication encompasses work across various disciplines within biology, with a particular emphasis on investigating the intersections between cell biology, developmental biology, and other related fields. Our primary objective is to present research conducted through a cell biological perspective, addressing the essential mechanisms governing cell function, cellular interactions, and responses to the environment. Moreover, we focus on understanding the collective behavior of cells, culminating in the formation of tissues, organs, and whole organisms, while also investigating the consequences of any malfunctions in these intricate processes.
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