Nuran Gökdere, Suna Sibel Rizvanoğlu, Erdal Emir, Hayrani Eren Bostanci, Müjde Eryilmaz, Orhan Atakol, İsmail Murat Palabiyik
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According to the results of antibacterial activity tests performed using the broth dilution method, ZnO NPs were found to be effective against <i>Staphylococcus aureus</i> ATCC 29213 (MSSA) and <i>S. aureus</i> ATCC 43300 (MRSA), and AgNPs were found to be effective against <i>Pseudomonas aeruginosa</i> ATCC 27853. Besides, AgNPs and ZnO NPs showed 88.5% and 39.98% antibiofilm activity, respectively. The synthesized nanoparticles did not show any antiquorum sensing activity. L929 (mouse fibroblast cell line) used as a control healthy cell line, and MCF7 (breast cancer), C6 (rat glioblastoma), and HT-29 (colon cancer) cells were used in cell culture studies as cancer cell lines. As a result of the cell culture studies, it was determined that the two nanoparticles had cytotoxic effects on both the MCF7 and HT29 cell lines. However, both nanoparticles did not show a cytotoxic effect on glioma cells. Flow cytometry studies were performed with the structures found to be active. Apoptosis and cell cycle studies also gave results parallel to the MTT results and proved the activity of the new synthetic structures. The findings indicate that AgNPs and ZnO NPs synthesized by <i>Pyracantha coccinea</i> M. Roem with an environmentally friendly approach can be evaluated as potential antimicrobial and anticancer agents in various biomedical applications.</p>\n </div>","PeriodicalId":8344,"journal":{"name":"Applied Organometallic Chemistry","volume":"39 10","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Green Synthesis and Characterization of Silver and Zinc Nanoparticles Using Pyracantha coccinea M. 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According to the results of antibacterial activity tests performed using the broth dilution method, ZnO NPs were found to be effective against <i>Staphylococcus aureus</i> ATCC 29213 (MSSA) and <i>S. aureus</i> ATCC 43300 (MRSA), and AgNPs were found to be effective against <i>Pseudomonas aeruginosa</i> ATCC 27853. Besides, AgNPs and ZnO NPs showed 88.5% and 39.98% antibiofilm activity, respectively. The synthesized nanoparticles did not show any antiquorum sensing activity. L929 (mouse fibroblast cell line) used as a control healthy cell line, and MCF7 (breast cancer), C6 (rat glioblastoma), and HT-29 (colon cancer) cells were used in cell culture studies as cancer cell lines. As a result of the cell culture studies, it was determined that the two nanoparticles had cytotoxic effects on both the MCF7 and HT29 cell lines. However, both nanoparticles did not show a cytotoxic effect on glioma cells. Flow cytometry studies were performed with the structures found to be active. Apoptosis and cell cycle studies also gave results parallel to the MTT results and proved the activity of the new synthetic structures. The findings indicate that AgNPs and ZnO NPs synthesized by <i>Pyracantha coccinea</i> M. 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Green Synthesis and Characterization of Silver and Zinc Nanoparticles Using Pyracantha coccinea M. Roem and Their Biological Activities
In this study, silver nanoparticles (AgNPs) and zinc nanoparticles (ZnO NPs) were synthesized using extracts prepared from the fruits of Pyracantha coccinea M. Roem with a green chemistry method. Synthesized nanoparticles were characterized using UV–visible spectrophotometry, scanning electron microscopy (SEM), X-ray diffraction technique (XRD), and Fourier transform infrared spectroscopy (FTIR). After characterization studies, antibacterial, antibiofilm, antiquorum sensing, and anticancer activities of AgNPs and ZnO NPs were investigated. According to the results of antibacterial activity tests performed using the broth dilution method, ZnO NPs were found to be effective against Staphylococcus aureus ATCC 29213 (MSSA) and S. aureus ATCC 43300 (MRSA), and AgNPs were found to be effective against Pseudomonas aeruginosa ATCC 27853. Besides, AgNPs and ZnO NPs showed 88.5% and 39.98% antibiofilm activity, respectively. The synthesized nanoparticles did not show any antiquorum sensing activity. L929 (mouse fibroblast cell line) used as a control healthy cell line, and MCF7 (breast cancer), C6 (rat glioblastoma), and HT-29 (colon cancer) cells were used in cell culture studies as cancer cell lines. As a result of the cell culture studies, it was determined that the two nanoparticles had cytotoxic effects on both the MCF7 and HT29 cell lines. However, both nanoparticles did not show a cytotoxic effect on glioma cells. Flow cytometry studies were performed with the structures found to be active. Apoptosis and cell cycle studies also gave results parallel to the MTT results and proved the activity of the new synthetic structures. The findings indicate that AgNPs and ZnO NPs synthesized by Pyracantha coccinea M. Roem with an environmentally friendly approach can be evaluated as potential antimicrobial and anticancer agents in various biomedical applications.
期刊介绍:
All new compounds should be satisfactorily identified and proof of their structure given according to generally accepted standards. Structural reports, such as papers exclusively dealing with synthesis and characterization, analytical techniques, or X-ray diffraction studies of metal-organic or organometallic compounds will not be considered. The editors reserve the right to refuse without peer review any manuscript that does not comply with the aims and scope of the journal. Applied Organometallic Chemistry publishes Full Papers, Reviews, Mini Reviews and Communications of scientific research in all areas of organometallic and metal-organic chemistry involving main group metals, transition metals, lanthanides and actinides. All contributions should contain an explicit application of novel compounds, for instance in materials science, nano science, catalysis, chemical vapour deposition, metal-mediated organic synthesis, polymers, bio-organometallics, metallo-therapy, metallo-diagnostics and medicine. Reviews of books covering aspects of the fields of focus are also published.