火棘银锌纳米粒子的绿色合成、表征及其生物活性研究

IF 3.7 2区 化学 Q2 CHEMISTRY, APPLIED
Nuran Gökdere, Suna Sibel Rizvanoğlu, Erdal Emir, Hayrani Eren Bostanci, Müjde Eryilmaz, Orhan Atakol, İsmail Murat Palabiyik
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引用次数: 0

摘要

本研究以火棘果为原料,采用绿色化学方法合成了银纳米粒子(AgNPs)和锌纳米粒子(ZnO NPs)。利用紫外可见分光光度法、扫描电镜(SEM)、x射线衍射技术(XRD)和傅里叶变换红外光谱(FTIR)对合成的纳米颗粒进行了表征。通过表征研究,研究了AgNPs和ZnO NPs的抗菌、抗生物膜、抗菌素感应和抗癌活性。采用肉汤稀释法进行抑菌活性试验,发现氧化锌NPs对金黄色葡萄球菌ATCC 29213 (MSSA)和金黄色葡萄球菌ATCC 43300 (MRSA)具有抑菌活性,AgNPs对铜绿假单胞菌ATCC 27853具有抑菌活性。AgNPs和ZnO NPs的抗菌活性分别为88.5%和39.98%。合成的纳米粒子没有表现出任何反聚量感应活性。L929(小鼠成纤维细胞系)作为对照健康细胞系,MCF7(乳腺癌)、C6(大鼠胶质母细胞瘤)和HT-29(结肠癌)细胞作为癌细胞系用于细胞培养研究。作为细胞培养研究的结果,确定这两种纳米颗粒对MCF7和HT29细胞系都有细胞毒性作用。然而,这两种纳米颗粒对胶质瘤细胞没有细胞毒性作用。流式细胞术研究发现有活性的结构。细胞凋亡和细胞周期研究也得到了与MTT结果相似的结果,证明了新合成结构的活性。研究结果表明,通过环境友好的方法合成的AgNPs和ZnO NPs可以作为潜在的抗菌和抗癌药物应用于各种生物医学领域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Green Synthesis and Characterization of Silver and Zinc Nanoparticles Using Pyracantha coccinea M. Roem and Their Biological Activities

Green Synthesis and Characterization of Silver and Zinc Nanoparticles Using Pyracantha coccinea M. Roem and Their Biological Activities

In this study, silver nanoparticles (AgNPs) and zinc nanoparticles (ZnO NPs) were synthesized using extracts prepared from the fruits of Pyracantha coccinea M. Roem with a green chemistry method. Synthesized nanoparticles were characterized using UV–visible spectrophotometry, scanning electron microscopy (SEM), X-ray diffraction technique (XRD), and Fourier transform infrared spectroscopy (FTIR). After characterization studies, antibacterial, antibiofilm, antiquorum sensing, and anticancer activities of AgNPs and ZnO NPs were investigated. According to the results of antibacterial activity tests performed using the broth dilution method, ZnO NPs were found to be effective against Staphylococcus aureus ATCC 29213 (MSSA) and S. aureus ATCC 43300 (MRSA), and AgNPs were found to be effective against Pseudomonas aeruginosa ATCC 27853. Besides, AgNPs and ZnO NPs showed 88.5% and 39.98% antibiofilm activity, respectively. The synthesized nanoparticles did not show any antiquorum sensing activity. L929 (mouse fibroblast cell line) used as a control healthy cell line, and MCF7 (breast cancer), C6 (rat glioblastoma), and HT-29 (colon cancer) cells were used in cell culture studies as cancer cell lines. As a result of the cell culture studies, it was determined that the two nanoparticles had cytotoxic effects on both the MCF7 and HT29 cell lines. However, both nanoparticles did not show a cytotoxic effect on glioma cells. Flow cytometry studies were performed with the structures found to be active. Apoptosis and cell cycle studies also gave results parallel to the MTT results and proved the activity of the new synthetic structures. The findings indicate that AgNPs and ZnO NPs synthesized by Pyracantha coccinea M. Roem with an environmentally friendly approach can be evaluated as potential antimicrobial and anticancer agents in various biomedical applications.

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来源期刊
Applied Organometallic Chemistry
Applied Organometallic Chemistry 化学-无机化学与核化学
CiteScore
7.80
自引率
10.30%
发文量
408
审稿时长
2.2 months
期刊介绍: All new compounds should be satisfactorily identified and proof of their structure given according to generally accepted standards. Structural reports, such as papers exclusively dealing with synthesis and characterization, analytical techniques, or X-ray diffraction studies of metal-organic or organometallic compounds will not be considered. The editors reserve the right to refuse without peer review any manuscript that does not comply with the aims and scope of the journal. Applied Organometallic Chemistry publishes Full Papers, Reviews, Mini Reviews and Communications of scientific research in all areas of organometallic and metal-organic chemistry involving main group metals, transition metals, lanthanides and actinides. All contributions should contain an explicit application of novel compounds, for instance in materials science, nano science, catalysis, chemical vapour deposition, metal-mediated organic synthesis, polymers, bio-organometallics, metallo-therapy, metallo-diagnostics and medicine. Reviews of books covering aspects of the fields of focus are also published.
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