活性部位作为碳水化合物酶的心脏

IF 1.7 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Russian Chemical Bulletin Pub Date : 2025-09-12 DOI:10.1007/s11172-025-4710-7

A. S. Dotsenko, A. M. Rozhkova, I. N. Zorov, O. A. Sinitsyna, S. A. Kurzeev, A. P. Sinitsyn

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引用次数: 0

摘要

通过对糖酶对各种多糖的催化活性的研究，以及活性位点或其附近氨基酸残基对酶的活性、酶的热稳定性、酶的最适pH值和对抑制剂的敏感性的影响，证实了糖酶活性位点空间排列的复杂性。在糖酶中引入氨基酸取代，可以提高酶的活性，提高多糖水解效率，提高酶的热稳定性和对抑制剂的抗性。

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Active site as the heart of carbohydrases

Studies of the catalytic activity of carbohydrases toward various polysaccharides and the data on the effect of the amino acid residues in the active site or in its vicinity on the activity of enzymes, their thermal stability, the pH optimum of the activity, and the susceptibility to inhibitors confirm the complexity of the spatial arrangement of their active site. The introduction of amino acid substitutions into carbohydrases made it possible to increase the activity of the enzymes, enhance the efficiency of polysaccharide hydrolysis, and increase the thermal stability of the enzymes and their resistance to inhibitors.

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来源期刊

Russian Chemical Bulletin 化学-化学综合

CiteScore

2.70

自引率

47.10%

发文量

257

审稿时长

3-8 weeks

期刊介绍： Publishing nearly 500 original articles a year, by leading Scientists from Russia and throughout the world, Russian Chemical Bulletin is a prominent international journal. The coverage of the journal spans practically all areas of fundamental chemical research and is presented in five sections: General and Inorganic Chemistry; Physical Chemistry; Organic Chemistry; Organometallic Chemistry; Chemistry of Natural Compounds and Bioorganic Chemistry.