Nanosized polymer-enzyme films based on poly(ionic liquids): design and application

Adsorption of poly(ionic liquid) (PIL) poly(1-ethyl-3-vinylimidazolium bromide) onto conductive surfaces was examined by quartz crystal microbalance with dissipation monitoring and atomic force microscopy. High surface coverage and the formation of continuous nanosized films were achieved by increasing the PIL concentration in the solution taken for adsorption of the polymer. The polymer-modified surfaces were used for subsequent electrostatic binding (adsorption) of glucose oxidase (GOx) as the model enzyme. The efficiency of the enzymatic reaction toward glucose for the prepared PIL—GOx thin films was evaluated by amperometry. The amperometric responses of the films correlate well with the efficiency of modification of the native surface by the polymer. Multiparameter physicochemical optimization of the adsorption of PIL and GOx made it possible to demonstrate the application potential of the polymer-enzyme thin films for highly sensitive analysis of glucose.