醇/盐双水相萃取法回收甘露糖赤藓醇脂质

IF 2.1 3区 工程技术 Q3 CHEMISTRY, MULTIDISCIPLINARY
Mellisa Z. Ncube, George M. Teke, Eugéne van Rensburg and Robert W. M. Pott*, 
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引用次数: 0

摘要

甘露糖赤藓糖醇脂(mel)是一种生物表面活性剂,在化妆品、制药和生物修复中有着广泛的应用。然而,使用油基的大规模生产使下游回收变得复杂,导致产量低,生产成本高。本研究探讨了水两相萃取(ATPE)作为一种可选的回收方法。使用乙醇、1-丙醇和2-丙醇与各种阴离子(磷酸盐、硫酸盐、柠檬酸盐和酒石酸盐)结合,在不同的pH、温度和盐浓度下,对醇/盐双水相体系(ATPS)进行了评价。1-丙醇/硫酸盐体系在富醇顶相中MEL回收率最高,达83.4%。添加2% (w/w) NaCl使MEL回收率提高到86.4%,而添加8% (w/w) NaCl使MEL回收率降低。由于马氏酵母可共同产生纤维素糖脂(CBLs),我们证明了乙醇/酒石酸盐体系可以有效地从CBLs中分离MELs。油酸是关键污染物,优先分配到顶相,表明进一步净化的必要性。为了验证该体系,利用1-丙醇/硫酸盐ATPS回收了黑穗病菌产生的mel,回收率为87.28%。这项研究首次展示了ATPE用于MEL的回收和从CBLs等副产物中分离,为工业生物表面活性剂生产中的传统纯化方法提供了一种有前途的可扩展替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Recovery of Mannosylerythritol Lipids Using Alcohol/Salt Aqueous Two-Phase Extraction

Mannosylerythritol lipids (MELs) are biosurfactants with applications in cosmetics, pharmaceuticals, and bioremediation. However, large-scale production using oil substrates complicates downstream recovery, resulting in low yields and high production costs. This study investigates aqueous two-phase extraction (ATPE) as an alternative recovery method. Alcohol/salt aqueous two-phase systems (ATPS) were evaluated using ethanol, 1-propanol, and 2-propanol in combination with various anions (phosphate, sulfate, citrate, and tartrate), while varying pH, temperature, and salt concentrations. The 1-propanol/sulfate system achieved the highest MEL recovery of 83.4% in the alcohol-rich top phase. The addition of 2% (w/w) NaCl improved recovery to 86.4%, while 8% (w/w) NaCl decreased MEL recovery. Since U. maydis coproduces cellobiose lipids (CBLs), we demonstrate that an ethanol/tartrate system could effectively separate MELs from CBLs. Oleic acid, a key contaminant, was found to preferentially partition into the top phase, indicating the necessity for further purification. To validate the system, MELs produced by Ustilago maydis were recovered using the 1-propanol/sulfate ATPS, yielding 87.28% recovery. This study is the first to demonstrate ATPE for MEL recovery and separation from coproducts such as CBLs, offering a promising and scalable alternative to conventional purification methods in industrial biosurfactant production.

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来源期刊
Journal of Chemical & Engineering Data
Journal of Chemical & Engineering Data 工程技术-工程:化工
CiteScore
5.20
自引率
19.20%
发文量
324
审稿时长
2.2 months
期刊介绍: The Journal of Chemical & Engineering Data is a monthly journal devoted to the publication of data obtained from both experiment and computation, which are viewed as complementary. It is the only American Chemical Society journal primarily concerned with articles containing data on the phase behavior and the physical, thermodynamic, and transport properties of well-defined materials, including complex mixtures of known compositions. While environmental and biological samples are of interest, their compositions must be known and reproducible. As a result, adsorption on natural product materials does not generally fit within the scope of Journal of Chemical & Engineering Data.
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