Faith A. Omonijo, Mengqi Wang, David Gagné, Mario Laterrière, Samuel Genier, Xin Zhao, Eveline M. Ibeagha-Awemu
{"title":"小核仁RNA失调及其在牛亚临床乳腺炎中的潜在作用","authors":"Faith A. Omonijo, Mengqi Wang, David Gagné, Mario Laterrière, Samuel Genier, Xin Zhao, Eveline M. Ibeagha-Awemu","doi":"10.1186/s40104-025-01230-9","DOIUrl":null,"url":null,"abstract":"Subclinical mastitis, caused by many pathogens including Staphylococcus aureus (S. aureus) and Staphylococcus chromogenes (S. chromogenes), presents a major challenge to the dairy industry due to its associated economic losses and poor milk quality. The molecular regulatory mechanisms, including the role of small nucleolar RNAs (snoRNAs), of the host response to mastitis pathogens remain unclear. Therefore, this study investigated snoRNA expression and potential roles during subclinical mastitis. Milk somatic cells from cows with naturally occurring S. aureus (n = 14) and S. chromogenes (n = 3) subclinical mastitis, and healthy cows (n = 4) were subjected to transcriptome sequencing and bioinformatics analyses. We identified 255 expressed snoRNAs including 21 differentially expressed (DE) in S. aureus-positive cows and 20 DE in S. chromogenes-positive cows. Prediction of ribosomal RNA (rRNA) modification sites found several 18S rRNA and 28S rRNA modification (pseudouridylation and 2′-O-methylation) target sites essential for ribosome function for DE snoRNAs, such as SNORA79 (18S-1319, 28S-3001), SNORA1 (18S-1496, 28S-1747), suggesting their roles in translation and immune modulation during subclinical mastitis. Correlation analysis identified DE snoRNAs-mRNAs (from the same samples) pairs with majority of the correlated mRNAs (e.g., CXCL8, IL6R, IL2, IL1R, IL18R1, STAT3, NFKB2, MYD88, VEGFA, and CD40) having immune related functions. Functional enrichment of correlated genes of snoRNAs for S. aureus-positive group (regulation of defense/immune response, leukocyte differentiation, response to cytokine, NF-κB signaling pathway, JAK-STAT signaling pathway etc.) and S. chromogenes-positive group (e.g., regulation of defense response, response to cytokine, regulation of immune response, NF-κB signaling pathway, TNF signaling pathway, and JAK-STAT signaling pathway) revealed involvement in immune and inflammatory processes. Some functional terms were common to both pathogens (e.g., NF-κB, JAK-STAT signaling, immune system processes) and suggest common regulatory mechanisms used by both pathogens to contain infection. Furthermore, snoRNA-mRNA network construction identified 7 key (hub) snoRNAs each for S. aureus-positive group (SNORA66, novelsnoRNA_26_14905 (also denoted as novelSnoRNA_86), SNORD107, SNORA1, SNORA63, SNORA79, SNORA76) and S. chromogenes-positive group (SNORD18, SNORA79, SNORA46, U2-19, SNORA66, SNORD37, SNORD49) that correlated with the most protein coding genes (|r| > 0.9; ≥ 30 mRNAs). Functional enrichment of correlated genes of hub snoRNAs reveals their involvement in immune related functions (75% of enriched terms) and metabolic processes (20% of enriched terms). These data suggest potential regulatory roles for the DE snoRNAs and in particular, the 14 hub snoRNAs during subclinical mastitis. This study presents the first evidence linking snoRNAs to bovine subclinical mastitis and offers new insights into the molecular mechanisms underlying subclinical mastitis caused by S. aureus and S. chromogenes.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"66 1","pages":""},"PeriodicalIF":6.5000,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Small nucleolar RNA dysregulation and potential roles in bovine subclinical mastitis\",\"authors\":\"Faith A. Omonijo, Mengqi Wang, David Gagné, Mario Laterrière, Samuel Genier, Xin Zhao, Eveline M. Ibeagha-Awemu\",\"doi\":\"10.1186/s40104-025-01230-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Subclinical mastitis, caused by many pathogens including Staphylococcus aureus (S. aureus) and Staphylococcus chromogenes (S. chromogenes), presents a major challenge to the dairy industry due to its associated economic losses and poor milk quality. The molecular regulatory mechanisms, including the role of small nucleolar RNAs (snoRNAs), of the host response to mastitis pathogens remain unclear. Therefore, this study investigated snoRNA expression and potential roles during subclinical mastitis. Milk somatic cells from cows with naturally occurring S. aureus (n = 14) and S. chromogenes (n = 3) subclinical mastitis, and healthy cows (n = 4) were subjected to transcriptome sequencing and bioinformatics analyses. We identified 255 expressed snoRNAs including 21 differentially expressed (DE) in S. aureus-positive cows and 20 DE in S. chromogenes-positive cows. Prediction of ribosomal RNA (rRNA) modification sites found several 18S rRNA and 28S rRNA modification (pseudouridylation and 2′-O-methylation) target sites essential for ribosome function for DE snoRNAs, such as SNORA79 (18S-1319, 28S-3001), SNORA1 (18S-1496, 28S-1747), suggesting their roles in translation and immune modulation during subclinical mastitis. Correlation analysis identified DE snoRNAs-mRNAs (from the same samples) pairs with majority of the correlated mRNAs (e.g., CXCL8, IL6R, IL2, IL1R, IL18R1, STAT3, NFKB2, MYD88, VEGFA, and CD40) having immune related functions. Functional enrichment of correlated genes of snoRNAs for S. aureus-positive group (regulation of defense/immune response, leukocyte differentiation, response to cytokine, NF-κB signaling pathway, JAK-STAT signaling pathway etc.) and S. chromogenes-positive group (e.g., regulation of defense response, response to cytokine, regulation of immune response, NF-κB signaling pathway, TNF signaling pathway, and JAK-STAT signaling pathway) revealed involvement in immune and inflammatory processes. Some functional terms were common to both pathogens (e.g., NF-κB, JAK-STAT signaling, immune system processes) and suggest common regulatory mechanisms used by both pathogens to contain infection. Furthermore, snoRNA-mRNA network construction identified 7 key (hub) snoRNAs each for S. aureus-positive group (SNORA66, novelsnoRNA_26_14905 (also denoted as novelSnoRNA_86), SNORD107, SNORA1, SNORA63, SNORA79, SNORA76) and S. chromogenes-positive group (SNORD18, SNORA79, SNORA46, U2-19, SNORA66, SNORD37, SNORD49) that correlated with the most protein coding genes (|r| > 0.9; ≥ 30 mRNAs). Functional enrichment of correlated genes of hub snoRNAs reveals their involvement in immune related functions (75% of enriched terms) and metabolic processes (20% of enriched terms). These data suggest potential regulatory roles for the DE snoRNAs and in particular, the 14 hub snoRNAs during subclinical mastitis. This study presents the first evidence linking snoRNAs to bovine subclinical mastitis and offers new insights into the molecular mechanisms underlying subclinical mastitis caused by S. aureus and S. chromogenes.\",\"PeriodicalId\":14928,\"journal\":{\"name\":\"Journal of Animal Science and Biotechnology\",\"volume\":\"66 1\",\"pages\":\"\"},\"PeriodicalIF\":6.5000,\"publicationDate\":\"2025-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Animal Science and Biotechnology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1186/s40104-025-01230-9\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Animal Science and Biotechnology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1186/s40104-025-01230-9","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Small nucleolar RNA dysregulation and potential roles in bovine subclinical mastitis
Subclinical mastitis, caused by many pathogens including Staphylococcus aureus (S. aureus) and Staphylococcus chromogenes (S. chromogenes), presents a major challenge to the dairy industry due to its associated economic losses and poor milk quality. The molecular regulatory mechanisms, including the role of small nucleolar RNAs (snoRNAs), of the host response to mastitis pathogens remain unclear. Therefore, this study investigated snoRNA expression and potential roles during subclinical mastitis. Milk somatic cells from cows with naturally occurring S. aureus (n = 14) and S. chromogenes (n = 3) subclinical mastitis, and healthy cows (n = 4) were subjected to transcriptome sequencing and bioinformatics analyses. We identified 255 expressed snoRNAs including 21 differentially expressed (DE) in S. aureus-positive cows and 20 DE in S. chromogenes-positive cows. Prediction of ribosomal RNA (rRNA) modification sites found several 18S rRNA and 28S rRNA modification (pseudouridylation and 2′-O-methylation) target sites essential for ribosome function for DE snoRNAs, such as SNORA79 (18S-1319, 28S-3001), SNORA1 (18S-1496, 28S-1747), suggesting their roles in translation and immune modulation during subclinical mastitis. Correlation analysis identified DE snoRNAs-mRNAs (from the same samples) pairs with majority of the correlated mRNAs (e.g., CXCL8, IL6R, IL2, IL1R, IL18R1, STAT3, NFKB2, MYD88, VEGFA, and CD40) having immune related functions. Functional enrichment of correlated genes of snoRNAs for S. aureus-positive group (regulation of defense/immune response, leukocyte differentiation, response to cytokine, NF-κB signaling pathway, JAK-STAT signaling pathway etc.) and S. chromogenes-positive group (e.g., regulation of defense response, response to cytokine, regulation of immune response, NF-κB signaling pathway, TNF signaling pathway, and JAK-STAT signaling pathway) revealed involvement in immune and inflammatory processes. Some functional terms were common to both pathogens (e.g., NF-κB, JAK-STAT signaling, immune system processes) and suggest common regulatory mechanisms used by both pathogens to contain infection. Furthermore, snoRNA-mRNA network construction identified 7 key (hub) snoRNAs each for S. aureus-positive group (SNORA66, novelsnoRNA_26_14905 (also denoted as novelSnoRNA_86), SNORD107, SNORA1, SNORA63, SNORA79, SNORA76) and S. chromogenes-positive group (SNORD18, SNORA79, SNORA46, U2-19, SNORA66, SNORD37, SNORD49) that correlated with the most protein coding genes (|r| > 0.9; ≥ 30 mRNAs). Functional enrichment of correlated genes of hub snoRNAs reveals their involvement in immune related functions (75% of enriched terms) and metabolic processes (20% of enriched terms). These data suggest potential regulatory roles for the DE snoRNAs and in particular, the 14 hub snoRNAs during subclinical mastitis. This study presents the first evidence linking snoRNAs to bovine subclinical mastitis and offers new insights into the molecular mechanisms underlying subclinical mastitis caused by S. aureus and S. chromogenes.
期刊介绍:
Journal of Animal Science and Biotechnology is an open access, peer-reviewed journal that encompasses all aspects of animal science and biotechnology. That includes domestic animal production, animal genetics and breeding, animal reproduction and physiology, animal nutrition and biochemistry, feed processing technology and bioevaluation, animal biotechnology, and meat science.