CXCR5的表达和基因多态性与腹腔镜胆囊切除术患者术后切口疼痛相关

IF 1.1 4区 医学 Q3 SURGERY
Lan Zhao, Yue Sun, Zengzhen Zhang, Huiqing Li, Xiaobo Fu
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引用次数: 0

摘要

背景:本研究旨在确定与急性胆囊炎腹腔镜胆囊切除术(ACC-LC)患者术后切口疼痛相关的生物标志物。方法:选取60例ACC-LC患者,根据术后24小时视觉模拟评分(VAS)分为轻度疼痛(MP)组和中重度疼痛(MSP)组。采用RNA测序筛选潜在的疼痛相关标志物,并采用ELISA法分析鉴定出的一种标志物CXCR5在外周血单核细胞(PBMCs)中的表达。对CXCR5 rs3922进行单核苷酸多态性基因分型,并评估其与疼痛水平、炎症标志物和围手术期临床特征的相关性。结果:与MP组相比,MSP组CXCR5表达明显上调。较高的CXCR5水平与VAS评分升高相关,并可预测疼痛严重程度。CXCR5 rs3922 G等位基因与CXCR5相关疼痛水平升高、白介素-6 (IL-6)水平升高、转化生长因子-β (tgf-β)水平降低相关。GG基因型患者还表现出较高的谷草转氨酶(AST)和丙氨酸转氨酶(ALT)水平,表明肝损害更大,肛门排气时间更长,术后并发症更多。结论:CXCR5表达和rs3922多态性与ACC-LC患者的切口疼痛和炎症损伤相关。这些发现表明,CXCR5可能作为疼痛预测和个性化疼痛管理策略的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression and Genetic Polymorphisms of CXCR5 Are Associated with Postoperative Incisional Pain in Patients Undergoing Laparoscopic Cholecystectomy.

Background: This study aimed to identify the biomarkers that was associated with the postoperative incisional pain in patients with acute cholecystitis undergoing laparoscopic cholecystectomy surgery (ACC-LC). Methods: Sixty ACC-LC patients were enrolled and divided into mild pain (MP) and moderate-to-severe pain (MSP) groups based on their visual analog scale (VAS) scores 24 hours postoperatively. RNA sequencing was used to screen the potential pain associated markers, and ELISA were used to analyze the expression of one identified marker, CXCR5 in peripheral blood mononuclear cells (PBMCs). Single nucleotide polymorphism genotyping for CXCR5 rs3922 was performed, and its correlation with pain levels, inflammatory markers, and perioperative clinical features were assessed. Results: CXCR5 expression was significantly upregulated in the MSP group compared to the MP group. Higher CXCR5 levels correlated with increased VAS scores and were predictive of pain severity. The CXCR5 rs3922 G allele was associated with elevated CXCR5-associated pain levels, together with the increased Interleukin-6 (IL-6) levels, and decreased Transforming Growth Factor-beta (tgf-β) levels. Patients carrying the GG genotype also exhibited higher Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT) levels, indicating greater liver damage, and experienced a longer time to anal exhaust and more postoperative complications. Conclusion: CXCR5 expression and the rs3922 polymorphism were associated with incisional pain and inflammatory damage in ACC-LC patients. These findings suggest that CXCR5 may serve as a biomarker for pain prediction and personalized pain management strategies.

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来源期刊
CiteScore
2.90
自引率
0.00%
发文量
163
审稿时长
3 months
期刊介绍: Journal of Laparoendoscopic & Advanced Surgical Techniques (JLAST) is the leading international peer-reviewed journal for practicing surgeons who want to keep up with the latest thinking and advanced surgical technologies in laparoscopy, endoscopy, NOTES, and robotics. The Journal is ideally suited to surgeons who are early adopters of new technology and techniques. Recognizing that many new technologies and techniques have significant overlap with several surgical specialties, JLAST is the first journal to focus on these topics both in general and pediatric surgery, and includes other surgical subspecialties such as: urology, gynecologic surgery, thoracic surgery, and more.
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