TaEPFL1基因通过调控乙烯合成调控小麦雌蕊和雄蕊的发育。

IF 3.8 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Tianxun Nie, Yuhuan Guo, Youwei Yang, Naoki Yamamoto, Wenxuan Liu, Yichao Wu, Mingli Liao, Zhenyong Chen, Zhengsong Peng, Zaijun Yang
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引用次数: 0

摘要

花器官的正常发育对小麦的繁殖成功和产量至关重要。然而,调控小麦花器官发育的分子机制仍不甚清楚。在这项研究中,我们鉴定了TaEPFL1基因在小麦花器官发育中的作用及其与乙烯信号的关联。TaEPFL1在雌蕊突变体HTS-1的未成熟穗中高度表达,尤其是在雌蕊和雄蕊形成阶段。其表达对外源乙烯和乙烯抑制剂1-甲基环丙烯(1-MCP)均有响应。TaEPFL1在转基因小麦中的过表达会导致雄蕊缩短、雌蕊缺陷、雄性不育和完全生殖失败。组织学分析显示绒毡层降解延迟,提示程序性细胞死亡中断(PCD)。气相色谱分析显示,过表达taepfl1的细胞系乙烯的生成和释放量显著降低。在1-MCP处理的野生型植物中也观察到类似的花缺陷。转录组和qRT-PCR分析进一步证实了多个乙烯生物合成相关基因的下调,包括三个TaACO同源基因。这些结果表明,TaEPFL1通过抑制TaACO表达负向调控乙烯生物合成,从而损害花器官分化。我们提出了一个反馈模型,其中乙烯诱导TaEPFL1,而TaEPFL1反过来抑制乙烯的产生以维持激素稳态。该研究揭示了TaEPFL1与乙烯介导的花发育之间的新调控机制,为通过分子育种提高小麦的育性提供了新的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
TaEPFL1 gene controls the development of wheat pistils and stamens by regulating ethylene synthesis.

Proper development of floral organs is essential for reproductive success and grain yield in wheat. However, the molecular mechanisms regulating wheat floral organ development remain largely unknown. In this study, we characterized the role of the wheat TaEPFL1 gene in floral organ development and its association with ethylene signaling. TaEPFL1 was highly expressed in immature spikes of the pistillody mutant HTS-1, particularly during the pistil and stamen specification stages. Its expression was responsive to both exogenous ethylene and the ethylene inhibitor 1-Methylcyclopropene (1-MCP). Overexpression of TaEPFL1 in transgenic wheat led to shortened stamens, defective pistils, male sterility, and complete reproductive failure. Histological analysis revealed delayed tapetum degradation, indicating disrupted programmed cell death (PCD). Gas chromatography (GC) showed significantly reduced ethylene production and release in TaEPFL1-overexpressing lines. Similar floral defects were observed in wild-type plants treated with 1-MCP. Transcriptome and qRT-PCR analyses further confirmed downregulation of multiple ethylene biosynthesis-related genes, including three homologs of TaACO. These results suggest that TaEPFL1 negatively regulates ethylene biosynthesis by repressing TaACO expression, thereby impairing floral organ differentiation. We propose a feedback model in which ethylene induces TaEPFL1, which in turn suppresses ethylene production to maintain hormonal homeostasis. This study reveals a novel regulatory mechanism linking TaEPFL1 to ethylene-mediated floral development and provides new insights for improving wheat fertility through molecular breeding.

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来源期刊
Plant Molecular Biology
Plant Molecular Biology 生物-生化与分子生物学
自引率
2.00%
发文量
95
审稿时长
1.4 months
期刊介绍: Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.
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