含对甲酚和香肉豆酱纳米脂质体的杀虫效果。抗埃及伊蚊及白纹伊蚊精油。

IF 1.7 3区 农林科学 Q2 ENTOMOLOGY
Aamir Ali, Hafiz Muhammad Tahir, Muniba Tariq, Hooria Ashraf Khan, Ayesha Muzamil, Kiran Zainab, Asjid Ghaffar
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引用次数: 0

摘要

利用植物基生态友好型杀幼虫剂控制登革热病媒蚊子是以最小的环境毒性抑制登革热传播的关键。研究了含对甲酚和香肉豆酱的纳米脂质体的杀虫活性。精油(EO)对埃及伊蚊和白纹伊蚊(Skuse, 1894)幼虫有效。采用气相色谱-质谱法分析提取的精油成分。研究了对甲酚和香薰精油对幼虫乙酰胆碱酯酶和α-淀粉酶的体外和体内抑制活性。制备并鉴定了纳米脂质体对伊蚊4龄幼虫的杀虫活性。主要成分为α-蒎烯、sabinene、β-蒎烯、β-茶树烯、γ-萜烯、异戊二酚和肉豆蔻素。结果表明,对甲酚和香薷精油对α-淀粉酶均具有明显的抑制活性。此外,香薷精油还能抑制乙酰胆碱酯酶的活性。分子对接结果也证实了这些抑制活性。香肉豆丝精油EO和对甲酚及其纳米脂质体对伊蚊具有显著的杀幼虫活性。埃及伊蚊和伊蚊。蚊。对甲酚(13和16 mg/L)的LC50值均低于香薷精油(33和39 mg/L)。埃及伊蚊和伊蚊。分别为白纹伊蚊幼虫。纳米脂质体对对甲酚和香薷精油的包封率为78% ~ 82%。纳米脂质体的水动力直径小于155 nm。PDI和zeta电位值分别小于0.2和- 28 mV,证实了其均匀分布和稳定性。综上所述,香薰精油和对甲酚对伊蚊幼虫均具有显著的杀幼虫活性。香薷精油和对甲酚的纳米脂质体均质稳定,进一步提高了香薷精油和对甲酚的药效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Larvicidal Effects of Nanoliposomes Containing p-Cresol and Myristica fragrans Houtt. Essential Oil Against Aedes aegypti and Aedes albopictus Mosquitoes.

The control of dengue vector mosquitoes by utilizing plant-based eco-friendly larvicides is pivotal in suppressing the spread of dengue with minimum environmental toxicity. This study aimed to evaluate the larvicidal activity of nanoliposomes containing p-cresol and Myristica fragrans Houtt. essential oil (EO) against Aedes aegypti L. and Aedes albopictus (Skuse, 1894) larvae. The constituents of extracted EO were assessed by GC-MS analyses. The in vitro and in silico inhibitory activities of p-cresol and M. fragrans EO against acetylcholinesterase and α-amylase enzymes of Larvae were assessed. Nanoliposomes were prepared and characterized and their Larvicidal activities were analyzed against 4th instar Aedes larvae. Major constituents identified from EO were α-pinene, sabinene, β-pinene, β-phellandrene, γ-terpinene, isopulegol, and myristicin. The results showed that both p-cresol and M. fragrans EO possess significant inhibitory activity against α-amylase. Moreover, M. fragrans EO also inhibited the activity of acetylcholinesterase enzyme. The molecular docking results also confirmed these inhibitory activities. Myristica fragrans EO and p-cresol as well as their nanoliposomes showed significant larvicidal activities against Ae. aegypti and Ae. albopictus. The LC50 values of p-cresol (13 and 16 mg/L) were lower than M. fragrans EO (33 and 39 mg/L) against both Ae. aegypti and Ae. albopictus larvae, respectively. The encapsulation efficacy of nanoliposomes for p-cresol and M. fragrans EO was 78 to 82%. The hydrodynamic diameter of nanoliposomes was below 155 nm. The PDI and zeta potential values were Less than 0.2 and - 28 mV respectively, confirming the homogenous distribution and stability. Here, it is concluded that both M. fragrans EO and p-cresol possess significant larvicidal activities against Aedes larvae. The nanoliposomes of M. fragrans EO and p-cresol were stable and homogenous, and these have further improved the efficacy of M. fragrans EO and p-cresol.

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来源期刊
Neotropical Entomology
Neotropical Entomology 生物-昆虫学
CiteScore
3.30
自引率
5.60%
发文量
69
审稿时长
6-12 weeks
期刊介绍: Neotropical Entomology is a bimonthly journal, edited by the Sociedade Entomológica do Brasil (Entomological Society of Brazil) that publishes original articles produced by Brazilian and international experts in several subspecialties of entomology. These include bionomics, systematics, morphology, physiology, behavior, ecology, biological control, crop protection and acarology.
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