{"title":"整合素在体内黑色素细胞迁移中的作用。","authors":"Wenjun Deng, Guy Tanentzapf","doi":"10.1091/mbc.E25-02-0066","DOIUrl":null,"url":null,"abstract":"<p><p>During embryonic development, neural crest-derived melanoblasts, which are precursors of pigment-producing melanocytes, disperse throughout the skin by long-range cell migration that requires adhesion to the ECM. Members of the integrin family of cell-ECM adhesion receptors are thought to contribute to melanocyte migration <i>in vitro</i>. However, due to the functional redundancy between different integrin heterodimers, the precise role of integrins in melanoblast migration, as well as the mechanisms that regulate them in this process, especially in <i>in vivo</i> contexts, remain poorly understood. To address this, we utilize the existing transcriptomic databases to identify different integrin subunits that are specifically expressed in melanoblasts, melanocytes and melanoma cancer cell lines. We then use mouse embryonic skin explants combined with drug and small molecule-based perturbations to target different integrins as well as specific mechanisms that modulate integrin activity. Individual melanoblasts from live imaging movies are tracked using high-resolution, quantitative, automated analysis, and cell morphology, cell migration and actin-based protrusions are analyzed. Overall, we uncover the non-redundant roles of different integrin heterodimers and elucidate the function of outside-in integrin activation in melanoblasts. Finally, we describe the function played, <i>in vivo,</i> by integrin-mediated adhesion to specific ECM ligands during melanoblast migration. [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text].</p>","PeriodicalId":18735,"journal":{"name":"Molecular Biology of the Cell","volume":" ","pages":"mbcE25020066"},"PeriodicalIF":2.7000,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Defining the Role of Integrins in Melanoblast Migration <i>In Vivo</i>.\",\"authors\":\"Wenjun Deng, Guy Tanentzapf\",\"doi\":\"10.1091/mbc.E25-02-0066\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>During embryonic development, neural crest-derived melanoblasts, which are precursors of pigment-producing melanocytes, disperse throughout the skin by long-range cell migration that requires adhesion to the ECM. Members of the integrin family of cell-ECM adhesion receptors are thought to contribute to melanocyte migration <i>in vitro</i>. However, due to the functional redundancy between different integrin heterodimers, the precise role of integrins in melanoblast migration, as well as the mechanisms that regulate them in this process, especially in <i>in vivo</i> contexts, remain poorly understood. To address this, we utilize the existing transcriptomic databases to identify different integrin subunits that are specifically expressed in melanoblasts, melanocytes and melanoma cancer cell lines. We then use mouse embryonic skin explants combined with drug and small molecule-based perturbations to target different integrins as well as specific mechanisms that modulate integrin activity. Individual melanoblasts from live imaging movies are tracked using high-resolution, quantitative, automated analysis, and cell morphology, cell migration and actin-based protrusions are analyzed. Overall, we uncover the non-redundant roles of different integrin heterodimers and elucidate the function of outside-in integrin activation in melanoblasts. Finally, we describe the function played, <i>in vivo,</i> by integrin-mediated adhesion to specific ECM ligands during melanoblast migration. [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text].</p>\",\"PeriodicalId\":18735,\"journal\":{\"name\":\"Molecular Biology of the Cell\",\"volume\":\" \",\"pages\":\"mbcE25020066\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-09-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Biology of the Cell\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1091/mbc.E25-02-0066\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Biology of the Cell","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1091/mbc.E25-02-0066","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Defining the Role of Integrins in Melanoblast Migration In Vivo.
During embryonic development, neural crest-derived melanoblasts, which are precursors of pigment-producing melanocytes, disperse throughout the skin by long-range cell migration that requires adhesion to the ECM. Members of the integrin family of cell-ECM adhesion receptors are thought to contribute to melanocyte migration in vitro. However, due to the functional redundancy between different integrin heterodimers, the precise role of integrins in melanoblast migration, as well as the mechanisms that regulate them in this process, especially in in vivo contexts, remain poorly understood. To address this, we utilize the existing transcriptomic databases to identify different integrin subunits that are specifically expressed in melanoblasts, melanocytes and melanoma cancer cell lines. We then use mouse embryonic skin explants combined with drug and small molecule-based perturbations to target different integrins as well as specific mechanisms that modulate integrin activity. Individual melanoblasts from live imaging movies are tracked using high-resolution, quantitative, automated analysis, and cell morphology, cell migration and actin-based protrusions are analyzed. Overall, we uncover the non-redundant roles of different integrin heterodimers and elucidate the function of outside-in integrin activation in melanoblasts. Finally, we describe the function played, in vivo, by integrin-mediated adhesion to specific ECM ligands during melanoblast migration. [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text].
期刊介绍:
MBoC publishes research articles that present conceptual advances of broad interest and significance within all areas of cell, molecular, and developmental biology. We welcome manuscripts that describe advances with applications across topics including but not limited to: cell growth and division; nuclear and cytoskeletal processes; membrane trafficking and autophagy; organelle biology; quantitative cell biology; physical cell biology and mechanobiology; cell signaling; stem cell biology and development; cancer biology; cellular immunology and microbial pathogenesis; cellular neurobiology; prokaryotic cell biology; and cell biology of disease.