口服褪黑激素调节过熟虹鳟(Oncorhynchus mykiss)卵的凋亡相关基因表达并抵消早期受精下降。

IF 2.5 3区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mahya Jabbari, Amirreza Abed-Elmdoust, Mohammad Meysam Salahi Ardekani, Kamran Rezaei Tavabe, Hamid Farahmand
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引用次数: 0

摘要

在水产养殖中,由于产卵延迟而导致的卵细胞过度成熟,是由细胞凋亡引发的,严重影响了鱼类的早期受精。针对细胞凋亡途径提供了一种有希望的方法来提高生殖结果,特别是考虑到褪黑激素在细胞凋亡调节中的有争议的作用,本研究评估了口服褪黑激素在0、50和200 mg/kg的干饲料剂量下对虹鳟鱼(Oncorhynchus mykiss)卵母细胞中关键凋亡相关基因(Bcl-2、p53、caspase-9)表达的影响。排卵后立即(第0天)和排卵后7天老化(过熟)。利用实时荧光定量PCR技术对基因表达进行量化,并评估受精率以确定生殖结果。褪黑素显著上调抗凋亡基因Bcl-2,下调促凋亡基因p53和caspase-9。虽然50和200 mg剂量对p53和caspase-9的表达没有显著差异,但200 mg剂量对提高过成熟卵母细胞受精率的影响更为明显。而对照组在过熟期受精率急剧下降。与对照组相比,两种褪黑激素剂量都显著提高了受精率,剂量越高效果越好。这些结果表明,褪黑激素通过调节细胞凋亡途径和提高受精结果来减轻卵母细胞过度成熟的有害影响。补充褪黑素可能是水产养殖中延长卵母细胞活力和提高延迟产卵情况下生殖性能的实用方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oral melatonin administration modulates apoptosis-related gene expression and counteracts early fertilization decline in overripe rainbow trout (Oncorhynchus mykiss) eggs.

Overripening of ovulated oocytes, often resulting from spawning delays in aquaculture, is triggered by apoptosis and significantly compromises early fertilization in fish. Targeting apoptosis pathways offers a promising approach to enhance reproductive outcomes, particularly given the controversial role of melatonin in apoptosis regulation, this study assessed the impact of oral melatonin supplementation at doses of 0, 50, and 200 mg/kg of dry feed on the expression of key apoptosis-related genes (Bcl-2, p53, caspase-9) and fertilization percentages in rainbow trout (Oncorhynchus mykiss) oocytes at two post-ovulation time points: immediately post-ovulation (day 0) and after 7 days of post-ovulatory ageing (overripening). Gene expression was quantified using real-time PCR, and fertilization percentages were evaluated to determine reproductive outcomes. Melatonin treatment notably upregulated the anti-apoptotic gene Bcl-2 and downregulated the pro-apoptotic genes p53 and caspase-9. While no significant differences were detected between the 50 and 200 mg doses for p53 and caspase-9 expression, the 200 mg dose demonstrated a more pronounced effect on improving fertilization percentages in overripened oocytes. In contrast, fertilization percentages declined sharply in the control group during overripening. Both melatonin dosages significantly improved fertilization percentages compared to the control, with higher doses showing greater effectiveness. These results suggest that melatonin alleviates the detrimental effects of oocyte overripening by modulating apoptosis pathways and enhancing fertilization outcomes. Melatonin supplementation may be a practical approach in aquaculture to extend oocyte viability and improve reproductive performance in delayed spawning scenarios.

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来源期刊
Fish Physiology and Biochemistry
Fish Physiology and Biochemistry 农林科学-生化与分子生物学
CiteScore
5.60
自引率
6.90%
发文量
106
审稿时长
4 months
期刊介绍: Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.
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