[1-13C]2-氧戊二酸的合成及13C呼吸试验评估TCA循环通量

IF 1.3 4区医学 Q4 CHEMISTRY, MEDICINAL

Chemical & pharmaceutical bulletin Pub Date : 2025-01-01 DOI:10.1248/cpb.c25-00400

Hidemichi Mitome, Kiyoshi Miura, Tomihiro Miyada, Ginjiro Kato, Mieko Takenishi, Kumiko Ono, Nanami Torada, Honoka Kutsuna, Kazuki Akira

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引用次数: 0

摘要

尝试了几种合成[1-13C]2-氧戊二酸的方法，并以三甲基硅基13c -氰化物为原料，通过4步合成成功。将新合成的[1-13C]2-氧戊二酸、先前制备的[1'-13C]柠檬酸、[1-13C]乙酸作为对照药口服给药，对大鼠进行13c呼吸试验，并比较结果。结果表明，[1-13C]2-氧己二酸和[1'-13C]柠檬酸可作为评价TCA循环通量的潜在底物。

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Synthesis of [1-¹³C]2-Oxoglutaric Acid and ¹³C Breath Tests Designed to Assess TCA Cycle Flux.

Several approaches for synthesizing [1-¹³C]2-oxoglutaric acid were attempted, and the synthesis was successfully achieved in 4 steps from trimethylsilyl ¹³C-cyanide. The ¹³C-breath tests on rats were conducted by orally administering the newly synthesized [1-¹³C]2-oxoglutaric acid, the previously prepared [1'-¹³C]citric acid, and [1-¹³C]acetic acid as a control drug, and the results were compared. The results indicate that [1-¹³C]2-oxoglutaric acid and [1'-¹³C]citric acid may serve as potential substrates for assessing the TCA cycle flux.

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来源期刊

Chemical & pharmaceutical bulletin 医学-化学综合

CiteScore

3.20

自引率

5.90%

发文量

132

审稿时长

1.7 months

期刊介绍： The CPB covers various chemical topics in the pharmaceutical and health sciences fields dealing with biologically active compounds, natural products, and medicines, while BPB deals with a wide range of biological topics in the pharmaceutical and health sciences fields including scientific research from basic to clinical studies. For details of their respective scopes, please refer to the submission topic categories below. Topics: Organic chemistry In silico science Inorganic chemistry Pharmacognosy Health statistics Forensic science Biochemistry Pharmacology Pharmaceutical care and science Medicinal chemistry Analytical chemistry Physical pharmacy Natural product chemistry Toxicology Environmental science Molecular and cellular biology Biopharmacy and pharmacokinetics Pharmaceutical education Chemical biology Physical chemistry Pharmaceutical engineering Epidemiology Hygiene Regulatory science Immunology and microbiology Clinical pharmacy Miscellaneous.