Mario Campana, Silvia Dante, Paola Astolfi, Simone Ranieri, Michela Pisani
{"title":"实时动力学如何解释立方体和六体纳米颗粒与脂质膜的不同相互作用","authors":"Mario Campana, Silvia Dante, Paola Astolfi, Simone Ranieri, Michela Pisani","doi":"10.1002/admi.202500186","DOIUrl":null,"url":null,"abstract":"<p>Cubosomes and hexosomes are interesting structures with diverse applications as drug and gene delivery systems, as well as theranostics. Understanding the uptake mechanism of these nanostructures by cell membranes is fundamental to improving and broadening their application. In this study, the kinetics of interaction of monoolein (GMO) cubosomes or dioleoylphosphatidylethanolamine (DOPE) hexosomes with supported lipid bilayers (SLBs) with varying compositions and stiffness, as valuable model membranes, are investigated by neutron reflectivity (NR) coupled with quartz crystal microbalance with dissipation monitoring (QCM-D). Atomic force microscopy (AFM) is also used to analyze SLBs topography after such interaction. Cubosomes rapidly interact with SLBs, resulting in lipid exchange between the two systems, destabilizing the bilayer, and ultimately causing its removal. The inclusion of cholesterol (CHOL) in the SLB confers rigidity, making it more resistant to removal. Completely different results are obtained when hexosomes interact with SLBs: an intrinsic resistance of the bilayers toward these nanoparticles is observed, regardless of CHOL presence, combined with a slight change in SLB composition, suggesting that hexosomes tend to fuse with the SLB. These findings provide new and valuable insights into the potential mechanisms and kinetics of cellular uptake of these systems, enhancing their potential as drug delivery vectors.</p>","PeriodicalId":115,"journal":{"name":"Advanced Materials Interfaces","volume":"12 17","pages":""},"PeriodicalIF":4.4000,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/admi.202500186","citationCount":"0","resultStr":"{\"title\":\"How Real-Time Kinetics Explain the Different Interaction of Cubosomes and Hexosomes Nanoparticles with Lipid Membranes\",\"authors\":\"Mario Campana, Silvia Dante, Paola Astolfi, Simone Ranieri, Michela Pisani\",\"doi\":\"10.1002/admi.202500186\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Cubosomes and hexosomes are interesting structures with diverse applications as drug and gene delivery systems, as well as theranostics. Understanding the uptake mechanism of these nanostructures by cell membranes is fundamental to improving and broadening their application. In this study, the kinetics of interaction of monoolein (GMO) cubosomes or dioleoylphosphatidylethanolamine (DOPE) hexosomes with supported lipid bilayers (SLBs) with varying compositions and stiffness, as valuable model membranes, are investigated by neutron reflectivity (NR) coupled with quartz crystal microbalance with dissipation monitoring (QCM-D). Atomic force microscopy (AFM) is also used to analyze SLBs topography after such interaction. Cubosomes rapidly interact with SLBs, resulting in lipid exchange between the two systems, destabilizing the bilayer, and ultimately causing its removal. The inclusion of cholesterol (CHOL) in the SLB confers rigidity, making it more resistant to removal. Completely different results are obtained when hexosomes interact with SLBs: an intrinsic resistance of the bilayers toward these nanoparticles is observed, regardless of CHOL presence, combined with a slight change in SLB composition, suggesting that hexosomes tend to fuse with the SLB. These findings provide new and valuable insights into the potential mechanisms and kinetics of cellular uptake of these systems, enhancing their potential as drug delivery vectors.</p>\",\"PeriodicalId\":115,\"journal\":{\"name\":\"Advanced Materials Interfaces\",\"volume\":\"12 17\",\"pages\":\"\"},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2025-07-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/admi.202500186\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced Materials Interfaces\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://advanced.onlinelibrary.wiley.com/doi/10.1002/admi.202500186\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Materials Interfaces","FirstCategoryId":"88","ListUrlMain":"https://advanced.onlinelibrary.wiley.com/doi/10.1002/admi.202500186","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
How Real-Time Kinetics Explain the Different Interaction of Cubosomes and Hexosomes Nanoparticles with Lipid Membranes
Cubosomes and hexosomes are interesting structures with diverse applications as drug and gene delivery systems, as well as theranostics. Understanding the uptake mechanism of these nanostructures by cell membranes is fundamental to improving and broadening their application. In this study, the kinetics of interaction of monoolein (GMO) cubosomes or dioleoylphosphatidylethanolamine (DOPE) hexosomes with supported lipid bilayers (SLBs) with varying compositions and stiffness, as valuable model membranes, are investigated by neutron reflectivity (NR) coupled with quartz crystal microbalance with dissipation monitoring (QCM-D). Atomic force microscopy (AFM) is also used to analyze SLBs topography after such interaction. Cubosomes rapidly interact with SLBs, resulting in lipid exchange between the two systems, destabilizing the bilayer, and ultimately causing its removal. The inclusion of cholesterol (CHOL) in the SLB confers rigidity, making it more resistant to removal. Completely different results are obtained when hexosomes interact with SLBs: an intrinsic resistance of the bilayers toward these nanoparticles is observed, regardless of CHOL presence, combined with a slight change in SLB composition, suggesting that hexosomes tend to fuse with the SLB. These findings provide new and valuable insights into the potential mechanisms and kinetics of cellular uptake of these systems, enhancing their potential as drug delivery vectors.
期刊介绍:
Advanced Materials Interfaces publishes top-level research on interface technologies and effects. Considering any interface formed between solids, liquids, and gases, the journal ensures an interdisciplinary blend of physics, chemistry, materials science, and life sciences. Advanced Materials Interfaces was launched in 2014 and received an Impact Factor of 4.834 in 2018.
The scope of Advanced Materials Interfaces is dedicated to interfaces and surfaces that play an essential role in virtually all materials and devices. Physics, chemistry, materials science and life sciences blend to encourage new, cross-pollinating ideas, which will drive forward our understanding of the processes at the interface.
Advanced Materials Interfaces covers all topics in interface-related research:
Oil / water separation,
Applications of nanostructured materials,
2D materials and heterostructures,
Surfaces and interfaces in organic electronic devices,
Catalysis and membranes,
Self-assembly and nanopatterned surfaces,
Composite and coating materials,
Biointerfaces for technical and medical applications.
Advanced Materials Interfaces provides a forum for topics on surface and interface science with a wide choice of formats: Reviews, Full Papers, and Communications, as well as Progress Reports and Research News.