猪卵泡液氧化应激指数影响体外培养过程中减数分裂成熟和胚胎发育。

IF 2 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Veterinary World Pub Date : 2025-07-01 Epub Date: 2025-07-27 DOI:10.14202/vetworld.2025.2078-2086
Bin Liu, Takeshige Otoi, Zhao Namula, Oky Setyo Widodo, Maki Hirata, Aya Nakai, Qingyi Lin, Yuichiro Nakayama, Megumi Nagahara, Fuminori Tanihara
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引用次数: 0

摘要

背景与目的:猪卵泡液(pFF)经常被用来模拟卵母细胞体外成熟(IVM)过程中的卵泡微环境。然而,pFF内氧化应激水平对卵母细胞质量和胚胎发育的影响尚不清楚。本研究旨在探讨不同氧化应激指数(OSI)对IVM期间猪卵母细胞减数分裂进程、受精和胚胎发育的影响。材料和方法:卵母细胞在添加30% pFF的IVM培养基中成熟,根据diacon -活性氧代谢物与生物抗氧化潜能的比值分为低(OSI 19)、中(OSI 22)和高(OSI 25)氧化应激组。ivm后,评估卵母细胞减数分裂阶段、DNA断裂、活性氧(ROS)和谷胱甘肽(GSH)水平。在体外受精和培养后监测受精和胚胎发育结果。结果:与OSI 22和OSI 25组相比,OSI 19组成熟至中期II期,受精率和囊胚形成率显著提高(p < 0.05)。在OSI 19卵母细胞中,ROS和GSH水平也显著升高,但DNA片段没有增加。OSI 25组囊胚DNA碎片化指数显著高于OSI 19组(p < 0.05)。结论:pFF的OSI调节着猪卵母细胞的能力和胚胎结局。较低的OSI与增强的抗氧化平衡、减数分裂成熟和胚胎质量有关。监测pFF氧化状态可以改善猪的辅助繁殖结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Oxidative stress index of porcine follicular fluid influences meiotic maturation and embryo development during <i>in vitro</i> culture.

Oxidative stress index of porcine follicular fluid influences meiotic maturation and embryo development during <i>in vitro</i> culture.

Oxidative stress index of porcine follicular fluid influences meiotic maturation and embryo development during in vitro culture.

Background and aim: Porcine follicular fluid (pFF) is frequently used to mimic the follicular microenvironment during in vitro maturation (IVM) of oocytes. However, the influence of oxidative stress levels within pFF on oocyte quality and embryo development remains unclear. This study aimed to investigate how varying oxidative stress index (OSI) of pFF affect porcine oocyte meiotic progression, fertilization, and embryonic development during IVM.

Materials and methods: Oocytes were matured in IVM media supplemented with 30% pFF classified into low (OSI 19), medium (OSI 22), and high (OSI 25) oxidative stress groups, based on the ratio of diacron-reactive oxygen metabolites to biological antioxidant potential. Post-IVM, oocytes were assessed for meiotic stage, DNA fragmentation, reactive oxygen species (ROS), and glutathione (GSH) levels. Fertilization and embryo development outcomes were monitored following in vitro fertilization and culture.

Results: The OSI 19 group showed significantly higher maturation to the metaphase II stage and improved fertilization and blastocyst formation rates compared to OSI 22 and OSI 25 groups (p < 0.05). ROS and GSH levels were also significantly elevated in OSI 19 oocytes, without an increase in DNA fragmentation. Blastocysts from the OSI 25 group exhibited significantly higher DNA fragmentation index than those from the OSI 19 group (p < 0.05).

Conclusion: The OSI of pFF modulates porcine oocyte competence and embryonic outcomes. Lower OSI is associated with enhanced antioxidant balance, meiotic maturation, and embryo quality. Monitoring pFF oxidative status may improve assisted reproductive outcomes in swine.

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来源期刊
Veterinary World
Veterinary World Multiple-
CiteScore
3.60
自引率
12.50%
发文量
317
审稿时长
16 weeks
期刊介绍: Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.
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