巴西人类和家畜多重耐药肺炎克雷伯菌克隆重叠和耐药谱:一项One Health分子流行病学研究

IF 2 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Veterinary World Pub Date : 2025-07-01 Epub Date: 2025-07-22 DOI:10.14202/vetworld.2025.2012-2023
Alessandra Tammy Hayakawa Ito de Sousa, Herica Makino, Marco Túlio Dos Santos Costa, Stefano Luis Cândido, Kaio Lierlyson Teles Gomes, Cristiane Silva Chitarra, Marco Andrey Pepato, Francisco Kennedy Scofoni Faleiros de Azevedo, Francisco Jose Dutra Souto, Arleana Bom Parto Ferreira de Almeida, Valeria Régia Franco Sousa, Luciano Nakazato, Valéria Dutra
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引用次数: 0

摘要

背景与目的:全球多药耐药肺炎克雷伯菌的上升对人类和动物健康构成严重威胁。人与家畜之间的密切接触可能促进耐多药菌株的人畜共患传播,强调需要采取综合监测战略。本研究旨在调查巴西马托格罗索州家畜和人类分离的肺炎克雷伯菌的遗传多样性、耐药机制和毒力基因谱。材料与方法:对48株临床分离株进行分析,其中动物分离株33株,人分离株15株。通过16S核糖体RNA测序证实了鉴定。采用纸片扩散法(动物分离株)和最低抑菌浓度法(人分离株)检测药物敏感性。采用聚合酶链反应检测耐药基因(bla kpc-2、bla NDM)和毒力基因(entB、fimH、wabG、ugE等)。对7个管家基因进行多位点序列分型(MLST),并使用巴斯德研究所数据库(巴黎,法国)分配序列类型(STs)。结果:70.83%(34/48)的分离株存在多药耐药表型,其中78.78%的动物和53%的人存在多药耐药表型。77.08%的分离株存在毒力基因;entB最常见(60.61%)。在3个人分离株中发现了bla kpc-2基因,在1个人和1个牛分离株中发现了bla NDM基因。MLST共发现39个STs,其中9个为新发现。克隆复合物(CC)258(人)、CC15(动物)和CC147(两个物种)表明可能存在种间传播。结论:本研究首次提供了马托格罗索州家畜和人肺炎克雷伯菌分子流行病学的综合快照。共享克隆复合物和耐多药高发生率的发现迫切需要在“同一个健康”方针下采取跨部门监测和控制战略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Clonal overlap and resistance profiles of multidrug-resistant <i>Klebsiella pneumoniae</i> in humans and domestic animals in Brazil: A One Health molecular epidemiology study.

Clonal overlap and resistance profiles of multidrug-resistant Klebsiella pneumoniae in humans and domestic animals in Brazil: A One Health molecular epidemiology study.

Background and aim: The global rise of multidrug-resistant (MDR) Klebsiella pneumoniae poses a serious threat to human and animal health. Close proximity between humans and domestic animals may facilitate zoonotic transmission of MDR strains, underscoring the need for integrated surveillance strategies. This study aimed to investigate the genetic diversity, resistance mechanisms, and virulence gene profiles of K. pneumoniae isolates from domestic animals and humans in Mato Grosso, Brazil, within the One Health framework.

Materials and methods: A total of 48 clinical isolates (33 from animals and 15 from humans) were analyzed. Identification was confirmed through 16S ribosomal RNA sequencing. Antimicrobial susceptibility was tested using disk diffusion (animal isolates) and minimum inhibitory concentration (human isolates). Resistance (bla kpc-2 and bla NDM) and virulence genes (entB, fimH, wabG, ugE, etc.) were detected through polymerase chain reaction. Multilocus sequence typing (MLST) was performed on seven housekeeping genes, and sequence types (STs) were assigned using the Pasteur Institute database (Paris, France).

Results: MDR phenotypes were found in 70.83% (34/48) of isolates - 78.78% of animal and 53% of human samples. Virulence genes were present in 77.08% of isolates; entB was the most prevalent (60.61%). The bla kpc-2 gene was found in three human isolates, and bla NDM was found in one human and one bovine isolate. MLST revealed 39 STs, including 9 novel ones. Clonal complexes (CC)258 (human), CC15 (animal), and CC147 (both species) indicated potential interspecies transmission.

Conclusion: This study provides the first comprehensive molecular epidemiological snapshot of K. pneumoniae in domestic animals and humans in Mato Grosso. The discovery of shared clonal complexes and high MDR rates demands urgent cross-sectoral surveillance and control strategies under the One Health approach.

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来源期刊
Veterinary World
Veterinary World Multiple-
CiteScore
3.60
自引率
12.50%
发文量
317
审稿时长
16 weeks
期刊介绍: Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.
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