牛瘦素基因中一种新的错义单核苷酸多态性(c.149G>A)及其与马杜拉牛生长性状的关系。

IF 2 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Veterinary World Pub Date : 2025-07-01 Epub Date: 2025-07-27 DOI:10.14202/vetworld.2025.2072-2077
Kuswati Kuswati, Irida Novianti, Rizki Prafitri, Wike Andre Septian, Rafika Febriani Putri, Chairdin Dwi Nugraha, Ahmad Furqon
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引用次数: 0

摘要

背景与目的:瘦素(LEP)基因在调节牛的生长、代谢和脂肪沉积中起关键作用。该基因的遗传多态性可以影响表型性状,并可作为育种计划中选择的分子标记。然而,对印尼当地品种(如Madura牛)LEP基因变异的全面表征仍然有限。本研究旨在鉴定牛LEP基因外显子2内新的单核苷酸多态性(SNPs),并评估其与马杜拉牛生长性状的关系。材料与方法:对45头2 ~ 4岁的马杜拉奶牛进行体重(BW)、退高(WH)、体长(BL)、胸围(CG)、臀高(HH)、头长(HL)、头宽(HW)的表型评价。提取基因组DNA,进行聚合酶链反应扩增,然后进行Sanger测序。采用一般线性模型分析检测到的snp的基因型和等位基因频率、Hardy-Weinberg平衡(HWE)及其与生长性状的关系。结果:在LEP基因外显子2中鉴定出3个SNP: C . 126t >C(同义)、C . 148t >C(错义)和一个新的错义SNP C . 149g > a,导致50位氨基酸上半胱氨酸到酪氨酸的替换。c.149G >a SNP多态性表现为GG、GA、AA三种基因型,其中GA基因型的杂合频率最高(64.4%)。该SNP偏离HWE (p < 0.05),表明潜在的选择压力或群体结构效应。虽然基因型与生长性状之间没有统计学上的显著相关性(p > 0.05),但AA基因型在大多数身体测量中显示出最高的平均值。结论:虽然新的c.149G >a SNP与生长性状无显著相关性,但其多态性和描述性性状模式提示其可能具有生物学相关性。建议进行更大规模的研究,以验证其作为马都拉牛生长发育遗传标记的效用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A novel missense single-nucleotide polymorphism (c.149G>A) in the bovine leptin gene and its association with growth traits in Madura cattle.

Background and aim: The leptin (LEP) gene plays a pivotal role in regulating growth, metabolism, and fat deposition in cattle. Genetic polymorphisms in this gene can influence phenotypic traits and may serve as molecular markers for selection in breeding programs. However, comprehensive characterization of LEP gene variants in local Indonesian breeds, such as Madura cattle, remains limited. This study aimed to identify novel single-nucleotide polymorphisms (SNPs) within exon 2 of the bovine LEP gene and assess their association with growth traits in Madura cattle.

Materials and methods: Forty-five Madura cows (aged 2-4 years) were phenotypically evaluated for body weight (BW), wither height (WH), body length (BL), chest girth (CG), hip height (HH), head length (HL), and head width (HW). Genomic DNA was extracted and subjected to polymerase chain reaction amplification followed by Sanger sequencing. Detected SNPs were analyzed for genotype and allele frequencies, Hardy-Weinberg equilibrium (HWE), and their associations with growth traits using a general linear model.

Results: Three SNPs were identified in exon 2 of the LEP gene: c.126T>C (synonymous), c.148T>C (missense), and a novel missense SNP c.149G>A, resulting in a cysteine to tyrosine substitution at amino acid position 50. The c.149G>A SNP showed polymorphism with three genotypes (GG, GA, AA), and the heterozygous GA genotype had the highest frequency (64.4%). This SNP deviated from HWE (p < 0.05), indicating potential selection pressure or population structure effects. While no statistically significant associations were found between genotypes and growth traits (p > 0.05), the AA genotype showed the highest mean values across most body measurements.

Conclusion: Although the novel c.149G>A SNP was not significantly associated with growth traits, its polymorphic nature and descriptive trait patterns suggest it may have biological relevance. Larger-scale studies are recommended to validate its utility as a genetic marker for growth and development in Madura cattle.

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来源期刊
Veterinary World
Veterinary World Multiple-
CiteScore
3.60
自引率
12.50%
发文量
317
审稿时长
16 weeks
期刊介绍: Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.
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