Tingting Mao, Shengjie Zhuo, Zigui Geng, Shengnan Wang, Yaru Zhang, Feifei Gao, Yang Yu, Wenlong Geng, Chunyan Yu, Bei Li, Juan Zhang, Hongxia Zhang
{"title":"细胞壁转化酶通过调节转基因大豆糖和激素代谢基因的表达来改善籽粒营养。","authors":"Tingting Mao, Shengjie Zhuo, Zigui Geng, Shengnan Wang, Yaru Zhang, Feifei Gao, Yang Yu, Wenlong Geng, Chunyan Yu, Bei Li, Juan Zhang, Hongxia Zhang","doi":"10.1093/aob/mcaf221","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and aims: </strong>Cell wall invertases have multiple roles in plant growth and development, yet their biological functions in seed oil production are still not understood.</p><p><strong>Methods: </strong>In the present study, the Oryza sativa (rice) cell wall invertase gene OsGIF1 (GRAIN INCOMPLETE FILLING 1) was ectopically expressed in Glycine max (Soybean) and its functions in grain yield and seed nutrition was investigated.</p><p><strong>Key results: </strong>We found that constitutive expression of OsGIF1 significantly improved biomass production, grain yield and seed nutrition in transgenic plants. The contents of protein and starch were significantly increased without dramatic alteration in the content of oil in the seeds of transgenic plants. In addition, the contents of histidine, tryptophan, asparagine, glutamine, palmitic acid and linoleic acid were increased, whereas the contents of oleic acid and linolenic acid were decreased. Further transcriptomic analyses in transgenic plants showed that genes associated with sugar and hormone metabolism were dramatically up-regulated.</p><p><strong>Conclusions: </strong>Our findings offer direct evidence for the potential usage of this gene in the genetic breeding of oil crops with improved grain yield and nutrition.</p>","PeriodicalId":8023,"journal":{"name":"Annals of botany","volume":" ","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cell wall invertase improves grain nutrition via regulating sugar and hormone metabolism gene expression in transgenic soybean.\",\"authors\":\"Tingting Mao, Shengjie Zhuo, Zigui Geng, Shengnan Wang, Yaru Zhang, Feifei Gao, Yang Yu, Wenlong Geng, Chunyan Yu, Bei Li, Juan Zhang, Hongxia Zhang\",\"doi\":\"10.1093/aob/mcaf221\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and aims: </strong>Cell wall invertases have multiple roles in plant growth and development, yet their biological functions in seed oil production are still not understood.</p><p><strong>Methods: </strong>In the present study, the Oryza sativa (rice) cell wall invertase gene OsGIF1 (GRAIN INCOMPLETE FILLING 1) was ectopically expressed in Glycine max (Soybean) and its functions in grain yield and seed nutrition was investigated.</p><p><strong>Key results: </strong>We found that constitutive expression of OsGIF1 significantly improved biomass production, grain yield and seed nutrition in transgenic plants. The contents of protein and starch were significantly increased without dramatic alteration in the content of oil in the seeds of transgenic plants. In addition, the contents of histidine, tryptophan, asparagine, glutamine, palmitic acid and linoleic acid were increased, whereas the contents of oleic acid and linolenic acid were decreased. Further transcriptomic analyses in transgenic plants showed that genes associated with sugar and hormone metabolism were dramatically up-regulated.</p><p><strong>Conclusions: </strong>Our findings offer direct evidence for the potential usage of this gene in the genetic breeding of oil crops with improved grain yield and nutrition.</p>\",\"PeriodicalId\":8023,\"journal\":{\"name\":\"Annals of botany\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2025-09-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of botany\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/aob/mcaf221\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of botany","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/aob/mcaf221","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Cell wall invertase improves grain nutrition via regulating sugar and hormone metabolism gene expression in transgenic soybean.
Background and aims: Cell wall invertases have multiple roles in plant growth and development, yet their biological functions in seed oil production are still not understood.
Methods: In the present study, the Oryza sativa (rice) cell wall invertase gene OsGIF1 (GRAIN INCOMPLETE FILLING 1) was ectopically expressed in Glycine max (Soybean) and its functions in grain yield and seed nutrition was investigated.
Key results: We found that constitutive expression of OsGIF1 significantly improved biomass production, grain yield and seed nutrition in transgenic plants. The contents of protein and starch were significantly increased without dramatic alteration in the content of oil in the seeds of transgenic plants. In addition, the contents of histidine, tryptophan, asparagine, glutamine, palmitic acid and linoleic acid were increased, whereas the contents of oleic acid and linolenic acid were decreased. Further transcriptomic analyses in transgenic plants showed that genes associated with sugar and hormone metabolism were dramatically up-regulated.
Conclusions: Our findings offer direct evidence for the potential usage of this gene in the genetic breeding of oil crops with improved grain yield and nutrition.
期刊介绍：
Annals of Botany is an international plant science journal publishing novel and rigorous research in all areas of plant science. It is published monthly in both electronic and printed forms with at least two extra issues each year that focus on a particular theme in plant biology. The Journal is managed by the Annals of Botany Company, a not-for-profit educational charity established to promote plant science worldwide.
The Journal publishes original research papers, invited and submitted review articles, ''Research in Context'' expanding on original work, ''Botanical Briefings'' as short overviews of important topics, and ''Viewpoints'' giving opinions. All papers in each issue are summarized briefly in Content Snapshots , there are topical news items in the Plant Cuttings section and Book Reviews . A rigorous review process ensures that readers are exposed to genuine and novel advances across a wide spectrum of botanical knowledge. All papers aim to advance knowledge and make a difference to our understanding of plant science.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
