病毒性肝炎作为喀麦隆黄热病疑似病例的鉴别诊断：患病率和分子特征

IF 1.7 Q4 INFECTIOUS DISEASES

IJID regions Pub Date : 2025-08-15 DOI:10.1016/j.ijregi.2025.100732

Abdou Fatawou Modiyinji , Huguette Tchetgna Simo , Aristide Mounchili-Njifon , Moise Henri Moumbeket-Yifomnjou , Lionel Franklin Djomo , Damaris Ngo Yabi , Justine Gwendolyne Odi , Gisèle Liliane Machuetum , Abanda Njei Ngu , Richard Njouom

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引用次数: 0

摘要

目的本研究的目的是确定初步怀疑为黄热病的急性发热性黄疸（YF）临床病例中病毒性肝炎的患病率和遗传多样性。方法对2024年9月至2025年1月采集的疑似yf阴性样本进行前瞻性横断面研究。采用五体聚合酶链反应法检测五种嗜肝病毒。所有检测到的病毒都进行了基因分型、测序和系统发育分析。结果在404名参与者中，107人被诊断为病毒性肝炎（26.5%；95%可信区间[CI]: 22.4-31.0%）。其中，甲型肝炎病毒（HAV）占70.1%(75/107)，乙型肝炎病毒（HBV）占23.4%(25/107)，丙型肝炎病毒（HCV）占3.7%(4/107)，戊型肝炎病毒（HEV）占2.8%（3/107）。样本未检出丁型肝炎病毒阳性，未检出双重病毒感染。甲型肝炎最常见于15岁以下的患者，而乙型肝炎最常见于15岁以上的患者。鉴定出的基因型为HAV-IB和HAV-IIA、HBV-A和E、HCV-1和HCV-4和HEV-4。结论我们的研究表明病毒性肝炎在喀麦隆的急性发热性黄疸病例中起关键作用，这些病例通常归因于YF病毒。

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Viral hepatitis as a differential diagnosis of yellow fever suspected cases in Cameroon: Prevalence and molecular characterization

Objectives

The aim of this study was to determine the prevalence and genetic diversity of viral hepatitis among clinical cases of acute febrile jaundice initially suspected to be yellow fever (YF).

Methods

We conducted a prospective cross-sectional study on YF-negative suspected samples collected between September 2024 and January 2025. The pentaplex polymerase chain reaction assay was used to detect five hepatotropic viruses. All detected viruses were genotyped, sequenced, and phylogenetically analyzed.

Results

Among the 404 participants tested, viral hepatitis was diagnosed in 107 (26.5%; 95% confidence interval [CI]: 22.4–31.0%). The proportions were: hepatitis A virus (HAV) 70.1% (75/107), hepatitis B virus (HBV) 23.4% (25/107), hepatitis C virus (HCV) 3.7% (4/107), and hepatitis E virus (HEV) 2.8% (3/107). No sample was positive for hepatitis D virus, and no dual viral infections were detected. HAV was most common in patients under 15 years of age, while HBV was most common in patients aged over 15. The genotypes identified were HAV-IB and HAV-IIA, HBV-A and E, HCV-1 and HCV-4, and HEV-4.