FPV在体内和体外均通过内在途径诱导pbmc细胞凋亡

IF 2 3区农林科学 Q4 IMMUNOLOGY

Comparative Immunology Microbiology and Infectious Diseases Pub Date : 2025-09-05 DOI:10.1016/j.cimid.2025.102402

Haibing Dong , Jiahui Li , Xinyue Zhang , Yongle Yu, Haiyan Yang, Chuanmei Zhang

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引用次数: 0

摘要

猫细小病毒（FPV）是一种典型的食肉动物细小病毒，可以感染猫科动物以及狐狸、浣熊和其他自然栖息地的动物。感染FPV的猫表现出白细胞减少的症状。然而，关于病因的文献报道有限。我们推测白细胞减少可能与FPV诱导PBMCs凋亡有关。在本研究中，我们通过IFA， CCK-8和qPCR探讨了FPV对PBMCs的影响。结果表明，FPV可降低细胞活力，加速细胞凋亡。培养细胞中内源性和外源性细胞因子的表达水平显著升高，体内pbmc中内源性凋亡相关细胞因子的表达水平升高。这些结果表明，FPV在体外可通过内源性和外源性途径诱导细胞凋亡，但在体内仅通过内源性途径诱导细胞凋亡。

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FPV induces apoptosis in PBMCs through intrinsic pathways both in vivo and in vitro

Feline parvovirus (FPV) is a typical carnivore parvovirus that infects felines as well as foxes, raccoons, and other animals in their natural habitats. Cats infected with FPV exhibit symptoms of leukopenia. However, there are limited literature reports on the cause. We hypothesized that leukopenia might be related to the induction of PBMCs apoptosis by FPV. In this study, we explored the effect of FPV on PBMCs by IFA, CCK-8, and qPCR. The results showed that FPV could decrease cell viability and accelerate cell apoptosis. The expression levels of Intrinsic and extrinsic cytokines were significantly increased in cultured cells, and the expression levels of Intrinsic apoptosis-related cytokines were increased in vivo PBMCs. These results indicate that FPV can induce apoptosis through both Intrinsic and extrinsic pathways in vitro, but only through Intrinsic pathways in vivo.

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来源期刊

Comparative Immunology Microbiology and Infectious Diseases 医学-免疫学

CiteScore

4.60

自引率

0.00%

发文量

102

审稿时长

40 days

期刊介绍： Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine . The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.