微/纳米塑料对豌豆生长和氮动态的影响：可生物降解塑料和常规塑料的作用

IF 5 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology Pub Date : 2025-09-09 DOI:10.1016/j.apsoil.2025.106445

Maoshan Xiong , Xu Zhou , Aiyun Guo , Jian Zhang

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引用次数: 0

摘要

与传统的微/纳米塑料（M/NPs）不同，可生物降解的M/NPs可以增加土壤中的碳含量并改变微生物功能，这可能会对豆科植物的氮调节产生不同的影响。迄今为止，它们对豌豆植物氮动态和植物生长的影响尚不清楚。因此，本研究旨在评估可生物降解和常规M/NPs在豌豆-土壤系统中氮转化和植物生长中的作用，并阐明控制氮动力学的潜在微生物机制。通过盆栽试验，系统研究了150 μm和500 nm两种粒径的常规塑料（聚丙烯和低密度聚乙烯）和生物降解塑料（PLA）对豌豆生长和土壤-豌豆氮素变化的影响。结果表明，PLA M/NPs具有显著的促生长作用，鲜重比对照提高46.89% ~ 98.54%，干重比对照提高30.41% ~ 104.96%，且效果较常规处理更为显著。微聚乳酸处理显著提高了土壤全氮和nh4 +-N含量，分别提高了10.86%和173.03%，纳米聚乳酸处理显著提高了20.84%和73.85%。宏基因组分析显示pla诱导了氮循环微生物群的重组，特别是纳米pla富集了固氮假单胞菌。功能基因分析表明，微聚乳酸显著上调固氮基因（nifK和nifH）。偏最小二乘路径模型表明，聚乳酸M/NPs通过改变土壤性质调控氮循环相关微生物群落和功能基因，最终影响豌豆生长。本研究揭示了聚乳酸M/NPs在促进土壤固氮和豌豆生长方面的意想不到的益处，为农业可持续发展和环境管理提供了新的视角。

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Effects of micro/nanoplastics on pea growth and nitrogen dynamics: The role of biodegradable and conventional plastics

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Effects of micro/nanoplastics on pea growth and nitrogen dynamics: The role of biodegradable and conventional plastics

Unlike conventional micro/nanoplastics (M/NPs), biodegradable M/NPs can increase carbon content in soil and alter microbial functionality, which may differentially affect nitrogen regulation in leguminous plants. To date, their impact on nitrogen dynamics and plant growth in pea plants remains unclear. Therefore, this study aims to assess the roles of biodegradable and conventional M/NPs in nitrogen transformation and plant growth within the pea-soil system, and to elucidate the underlying microbial mechanisms governing nitrogen dynamics. Through pot experiments, the effects of conventional plastics (polypropylene and low-density polyethylene) and biodegradable plastic (PLA), in two particle sizes (150 μm and 500 nm), on pea growth and soil-pea nitrogen variation were systematically studied. Results revealed that PLA M/NPs exhibited a growth-promoting effect, increasing fresh weight by 46.89 %–98.54 % and dry weight by 30.41 %–104.96 % compared to CK, with more pronounced effects than conventional plastics. Micro-PLA treatment significantly increased total nitrogen and NH₄⁺-N content in the soil by 10.86 % and 173.03 %, respectively, while nano-PLA treatment increased these two nitrogen forms by 20.84 % and 73.85 %, respectively. Metagenomic analysis revealed PLA-induced restructuring of nitrogen-cycling microbiota, particularly nano-PLA enrichment of nitrogen-fixing Pseudomonadota. Functional gene analysis demonstrated that micro-PLA significantly up-regulated nitrogen fixation genes (nifK and nifH). Partial least squares path models showed that PLA M/NPs regulated nitrogen cycling-related microbial communities and functional genes by altering soil properties and ultimately affected pea growth. This research reveals the unexpected benefits of PLA M/NPs in promoting soil nitrogen fixation and pea development, offering new perspectives for sustainable agricultural development and environmental management.

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来源期刊

Applied Soil Ecology 农林科学-土壤科学

CiteScore

9.70

自引率

4.20%

发文量

363

审稿时长

5.3 months

期刊介绍： Applied Soil Ecology addresses the role of soil organisms and their interactions in relation to: sustainability and productivity, nutrient cycling and other soil processes, the maintenance of soil functions, the impact of human activities on soil ecosystems and bio(techno)logical control of soil-inhabiting pests, diseases and weeds.