曲霉木霉在豇豆诱导系统抗性和抑制南枯萎病中的作用

IF 3.3 3区 农林科学 Q2 PLANT SCIENCES
Praveen Vijayakumar, Sudha Appusami
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引用次数: 0

摘要

豇豆种植受到南方枯萎病的严重影响,南方枯萎病是一种由土壤传播的病原菌罗氏病(Agroathelia rolfsii)引起的破坏性疾病。传统的化学控制方法不适合有机系统,并造成环境危害和抗性发展的风险。众所周知,木霉对各种土传病原体具有拮抗作用,并具有促进植物生长的能力。在本研究中,用双平板法对曲霉的病原菌进行了鉴定。开发了一种液体制剂,并通过种子生物启动、卷巾试验、酶活性测定、盆栽和田间试验来测试其功效。结果表明,t。曲霉对病原菌生长的抑制作用约为70.00 %。5 ml/kg种子处理使根长和芽长分别增加到19.42 cm和26.04 cm,发芽率100%,活力指数4546.00。淀粉酶和脱氢酶活性分别为12.42 mg麦芽糖和2.10 mg麦芽糖。在温室和田间条件下,与对照(80.82%)相比,处理植株的病害发生率显著降低(8.87%),生长和产量也得到改善。此外,处理过的植物对防御相关酶如过氧化物酶、多酚氧化酶和苯丙氨酸解氨酶的诱导程度较高。因此,该配方通过防御诱导有效地促进生长,抑制南方枯萎病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Functional role of Trichoderma asperellum in inducing systemic resistance and suppressing southern blight (Agroathelia rolfsii) in cowpea
Cowpea cultivation is severely impacted by southern blight, a destructive disease caused by the soil-borne pathogen Agroathelia rolfsii (syn. Sclerotium rolfsii). Conventional chemical control methods are unsuitable for organic systems and pose environmental hazards and risks of resistance development. Trichoderma spp. are well known for their antagonistic effects against various soil-borne pathogens and their ability to promote plant growth. In this study, Trichoderma asperellum was evaluated against the pathogen using a dual plate assay. A liquid formulation was developed, and its efficacy was tested through seed bio-priming, roll towel assays, enzyme activity measurement, pot culture, and field trials. The results showed that
T. asperellum inhibited about 70.00 % of pathogen growth. Seed treatment at 5 ml/kg enhanced root and shoot lengths to 19.42 cm and 26.04 cm respectively, with 100 % germination and a vigour index of 4546.00. Amylase and dehydrogenase activities were recorded as 12.42 mg maltose and 2.10, respectively. In greenhouse and field conditions, disease incidence was significantly reduced in treated plants (8.87 %) compared to control (80.82 %), along with improved growth and yield. Moreover, treated plants showed higher induction of defense-related enzymes like peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase. Thus, the formulation effectively enhances growth and suppresses southern blight through defense induction.
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来源期刊
CiteScore
4.30
自引率
7.40%
发文量
130
审稿时长
38 days
期刊介绍: Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.
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