{"title":"曲霉木霉在豇豆诱导系统抗性和抑制南枯萎病中的作用","authors":"Praveen Vijayakumar, Sudha Appusami","doi":"10.1016/j.pmpp.2025.102909","DOIUrl":null,"url":null,"abstract":"<div><div>Cowpea cultivation is severely impacted by southern blight, a destructive disease caused by the soil-borne pathogen <em>Agroathelia rolfsii</em> (syn. <em>Sclerotium rolfsii</em>). Conventional chemical control methods are unsuitable for organic systems and pose environmental hazards and risks of resistance development. <em>Trichoderma</em> spp. are well known for their antagonistic effects against various soil-borne pathogens and their ability to promote plant growth. In this study, <em>Trichoderma asperellum</em> was evaluated against the pathogen using a dual plate assay. A liquid formulation was developed, and its efficacy was tested through seed bio-priming, roll towel assays, enzyme activity measurement, pot culture, and field trials. The results showed that</div><div><em>T. asperellum</em> inhibited about 70.00 % of pathogen growth. Seed treatment at 5 ml/kg enhanced root and shoot lengths to 19.42 cm and 26.04 cm respectively, with 100 % germination and a vigour index of 4546.00. Amylase and dehydrogenase activities were recorded as 12.42 mg maltose and 2.10, respectively. In greenhouse and field conditions, disease incidence was significantly reduced in treated plants (8.87 %) compared to control (80.82 %), along with improved growth and yield. Moreover, treated plants showed higher induction of defense-related enzymes like peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase. Thus, the formulation effectively enhances growth and suppresses southern blight through defense induction.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102909"},"PeriodicalIF":3.3000,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Functional role of Trichoderma asperellum in inducing systemic resistance and suppressing southern blight (Agroathelia rolfsii) in cowpea\",\"authors\":\"Praveen Vijayakumar, Sudha Appusami\",\"doi\":\"10.1016/j.pmpp.2025.102909\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Cowpea cultivation is severely impacted by southern blight, a destructive disease caused by the soil-borne pathogen <em>Agroathelia rolfsii</em> (syn. <em>Sclerotium rolfsii</em>). Conventional chemical control methods are unsuitable for organic systems and pose environmental hazards and risks of resistance development. <em>Trichoderma</em> spp. are well known for their antagonistic effects against various soil-borne pathogens and their ability to promote plant growth. In this study, <em>Trichoderma asperellum</em> was evaluated against the pathogen using a dual plate assay. A liquid formulation was developed, and its efficacy was tested through seed bio-priming, roll towel assays, enzyme activity measurement, pot culture, and field trials. The results showed that</div><div><em>T. asperellum</em> inhibited about 70.00 % of pathogen growth. Seed treatment at 5 ml/kg enhanced root and shoot lengths to 19.42 cm and 26.04 cm respectively, with 100 % germination and a vigour index of 4546.00. Amylase and dehydrogenase activities were recorded as 12.42 mg maltose and 2.10, respectively. In greenhouse and field conditions, disease incidence was significantly reduced in treated plants (8.87 %) compared to control (80.82 %), along with improved growth and yield. Moreover, treated plants showed higher induction of defense-related enzymes like peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase. Thus, the formulation effectively enhances growth and suppresses southern blight through defense induction.</div></div>\",\"PeriodicalId\":20046,\"journal\":{\"name\":\"Physiological and Molecular Plant Pathology\",\"volume\":\"140 \",\"pages\":\"Article 102909\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2025-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physiological and Molecular Plant Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0885576525003480\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiological and Molecular Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0885576525003480","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Functional role of Trichoderma asperellum in inducing systemic resistance and suppressing southern blight (Agroathelia rolfsii) in cowpea
Cowpea cultivation is severely impacted by southern blight, a destructive disease caused by the soil-borne pathogen Agroathelia rolfsii (syn. Sclerotium rolfsii). Conventional chemical control methods are unsuitable for organic systems and pose environmental hazards and risks of resistance development. Trichoderma spp. are well known for their antagonistic effects against various soil-borne pathogens and their ability to promote plant growth. In this study, Trichoderma asperellum was evaluated against the pathogen using a dual plate assay. A liquid formulation was developed, and its efficacy was tested through seed bio-priming, roll towel assays, enzyme activity measurement, pot culture, and field trials. The results showed that
T. asperellum inhibited about 70.00 % of pathogen growth. Seed treatment at 5 ml/kg enhanced root and shoot lengths to 19.42 cm and 26.04 cm respectively, with 100 % germination and a vigour index of 4546.00. Amylase and dehydrogenase activities were recorded as 12.42 mg maltose and 2.10, respectively. In greenhouse and field conditions, disease incidence was significantly reduced in treated plants (8.87 %) compared to control (80.82 %), along with improved growth and yield. Moreover, treated plants showed higher induction of defense-related enzymes like peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase. Thus, the formulation effectively enhances growth and suppresses southern blight through defense induction.
期刊介绍:
Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions.
Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.