mirandae内生镰刀菌对番茄生长有促进作用,对病原菌有抑制作用,对番茄灰霉病有保护作用。

IF 2.1 4区 生物学 Q4 MICROBIOLOGY
Nidia Del C Ríos-De León, Miguel Á Salas-Marina, Vidal Hernández-García, Brenda Del R Saldaña-Morales, Luis A Rodríguez-Larramendi, Rubén Martínez-Camilo, Claudio Ríos-Velasco, Daniel A Pérez-Corral, Sergio Casas-Flores
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引用次数: 0

摘要

农作物真菌病害造成经济损失,化学防治是防治真菌病害的常规方法。然而,这种做法对环境和人类健康都产生了负面影响。本研究的重点是从墨西哥Juan Sabines (Villa Corzo, Chiapas)地区的mirandae Ceratozamia根部分离的内生真菌。对这些真菌进行了形态、分子、生化鉴定,并评价了它们对炭疽病菌、新拟多毛孢病菌和尖孢镰刀菌的拮抗活性。还评估了它们在促进拟南芥和番茄生长以及防止灰霉病方面的潜力。鉴定出14株分离真菌,分为6属:镰刀菌属、拟盘多毛菌属、木霉属、伞形菌属、黑孢菌属和足孢菌属。其中增殖镰刀菌JS311和尖孢镰刀菌JS239对被试病原菌有较强的抑制作用。8株菌株可产生吲哚-3-乙酸,促进拟南芥和番茄植株的生长。值得注意的是,F. oxysporum JS439和F. solani (JS240, JS256和JS4101)表现出额外的能力,包括在无氮培养基中产生铁载体和生长。所有内生镰刀菌分离株均对番茄灰霉病菌产生系统抗性。mirandae内生真菌具有抗植物病原活性、促进植物生长和通过产生有益代谢物诱导系统抗性的综合机制,因此作为生物肥料具有很大的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Endophytic Fusarium isolates from Ceratozamia mirandae enhance tomato growth, suppress pathogenic fungi, and induce protection against Botrytis cinerea.

Fungal diseases in agricultural crops cause economic losses, with chemical control being the conventional method to manage them. However, this approach negatively impacts both the environment and human health. This study focused on endophytic fungi isolated from the roots of Ceratozamia mirandae in the Mexican locality of Juan Sabines (Villa Corzo, Chiapas). These fungi were identified morphologically and molecularly, biochemically characterized, and evaluated for their antagonistic activity against Colletotrichum karstii, Neopestalotiopsis sp. and Fusarium oxysporum. Their potential for promoting growth in Arabidopsis thaliana and tomato, as well as protecting against Botrytis cinerea, was also assessed. Fourteen fungal isolates were identified and grouped into six genera: Fusarium, Pestalotiopsis, Trichoderma, Umbelopsis, Nectria and Podospora. Among these Fusarium proliferatum JS311 and F. oxysporum JS239 exhibited strong inhibitory effects against the tested pathogens. Eight isolates were found to produce indole-3-acetic acid, promoting the growth of A. thaliana and tomato plants. Notably, F. oxysporum JS439 and F. solani (JS240, JS256 and JS4101) exhibited additional capabilities, including siderophore production and growth in nitrogen-free media. All Fusarium endophytic isolates induced systemic resistance against B. cinerea in tomato. Endophytic fungi from C. mirandae show promising potential as biofertilizers due to their combined mechanisms of anti-phytopathogenic activity, plant growth promotion, and systemic resistance induction through the production of beneficial metabolites.

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来源期刊
CiteScore
3.30
自引率
0.00%
发文量
46
审稿时长
>12 weeks
期刊介绍: La Revista Argentina de Microbiología es una publicación trimestral editada por la Asociación Argentina de Microbiología y destinada a la difusión de trabajos científicos en las distintas áreas de la Microbiología. La Asociación Argentina de Microbiología se reserva los derechos de propiedad y reproducción del material aceptado y publicado.
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