电针诱导心肌缺血再灌注损伤通过fto依赖性m6A甲基化调节。

IF 1.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Open Medicine Pub Date : 2025-08-19 eCollection Date: 2025-01-01 DOI:10.1515/med-2025-1255
Ying-Hua Zou, En Zhou, Chen-Long Xie, Long Sun, Yu-Jiao Zhang, Chun-Chun Wang, Xing Li, Jun Guo
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引用次数: 0

摘要

目的:本研究旨在探讨电针通过调节脂肪量和肥胖相关蛋白(FTO)影响n6 -甲基腺苷(m6A)甲基化来减轻心肌缺血再灌注损伤(MIRI)的潜力。方法:通过手术阻断左冠状动脉前降支,再灌注建立小鼠MIRI实验模型。在缺血诱导前7天进行电针内关穴治疗。超声心动图评估心功能,Evans Blue和三苯四唑双染色评估心肌梗死大小。利用RNA免疫沉淀和定量聚合酶链反应测定m6A甲基化和FTO及中介复合物亚基1 (Med1) mRNA表达。Western blot检测Med1、Bcl-2、Bax蛋白表达水平。结果:电针预处理与心肌损伤的减少有关,这可以通过保留射血分数和减少梗死面积来证明。电针治疗后心肌组织FTO表达增强,m6A甲基化降低。此外,电针治疗组m6A的下游靶点Med1 mRNA表达降低,与心肌细胞凋亡减少相关。结论:电针预处理可能通过上调FTO,从而调节m6A甲基化,降低Med1表达,从而在MIRI中发挥心脏保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Electroacupuncture-induced reduction of myocardial ischemia-reperfusion injury via FTO-dependent m6A methylation modulation.

Electroacupuncture-induced reduction of myocardial ischemia-reperfusion injury via FTO-dependent m6A methylation modulation.

Electroacupuncture-induced reduction of myocardial ischemia-reperfusion injury via FTO-dependent m6A methylation modulation.

Electroacupuncture-induced reduction of myocardial ischemia-reperfusion injury via FTO-dependent m6A methylation modulation.

Objective: This study aims to investigate the potential of electroacupuncture to mitigate myocardial ischemia-reperfusion injury (MIRI) by influencing N6-methyladenosine (m6A) methylation through modulation of the fat mass and obesity-associated protein (FTO).

Methods: An experimental murine model of MIRI was established by surgically occluding the left anterior descending coronary artery, followed by reperfusion. Electroacupuncture treatment targeting Neiguan acupoints was administered 7 days before ischemia induction. Cardiac function was evaluated using echocardiography, and myocardial infarction size was assessed through Evans Blue and triphenyltetrazolium chloride dual staining. To measure m6A methylation and mRNA expression of FTO and mediator complex subunit 1 (Med1), RNA immunoprecipitation and quantitative polymerase chain reaction were utilized. Western blot analysis was conducted to determine the protein expression levels of Med1, Bcl-2, and Bax.

Results: Electroacupuncture pretreatment was associated with a reduction in myocardial injury, demonstrated by preserved ejection fraction and reduced infarct size. Enhanced FTO expression and decreased m6A methylation were observed in myocardial tissue following electroacupuncture treatment. Additionally, Med1 - a downstream target of m6A - exhibited decreased mRNA expression in the electroacupuncture-treated group, correlating with reduced cardiomyocyte apoptosis.

Conclusion: Electroacupuncture pretreatment may confer cardioprotective effects in MIRI by upregulating FTO, thereby modulating m6A methylation and reducing Med1 expression.

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来源期刊
Open Medicine
Open Medicine Medicine-General Medicine
CiteScore
3.00
自引率
0.00%
发文量
153
审稿时长
20 weeks
期刊介绍: Open Medicine is an open access journal that provides users with free, instant, and continued access to all content worldwide. The primary goal of the journal has always been a focus on maintaining the high quality of its published content. Its mission is to facilitate the exchange of ideas between medical science researchers from different countries. Papers connected to all fields of medicine and public health are welcomed. Open Medicine accepts submissions of research articles, reviews, case reports, letters to editor and book reviews.
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