白藜芦醇和紫檀芪的亲脂性、细胞膜通透性和细胞内抗氧化能力的比较研究。

IF 4.9 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Nutritional Biochemistry Pub Date : 2025-09-05 DOI:10.1016/j.jnutbio.2025.110095

Tengteng Huang, Xiaoling Chen, Daiwen Chen, Bing Yu, Hui Yan, Ping Zheng, Junqiu Luo, Zhiqing Huang

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引用次数: 0

摘要

紫檀芪（PTS）是白藜芦醇（RES）的甲基化类似物，具有优越的生物利用度，与RES相比，可能归因于增强的亲脂性和细胞膜通透性。然而，这一假设缺乏实验验证。本研究的目的是比较RES和PTS的亲脂性、细胞膜通透性和细胞内抗氧化能力。通过摇瓶法评估了PTS的亲脂性，结果显示PTS的亲脂性高于res。分子动力学模拟预测PTS的膜透性更强。使用cyanine2-labeled RES （CY2-RES）和PTS （CY2-PTS）对IPEC-J2细胞和猪肌管进行的细胞摄取研究证实了这些预测，荧光显微镜和流式细胞术一致显示，与CY2-RES相比，细胞内CY2-PTS的积累更高。此外，PTS在IPEC-J2细胞和猪肌管中表现出比RES更强的细胞内ros清除能力。总之，我们的研究表明，与RES相比，PTS具有更好的亲脂性，增强的膜通透性和更强的细胞内抗氧化能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Comparative study of lipophilicity, cell membrane permeability, and intracellular antioxidant capacity of resveratrol and pterostilbene

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Comparative study of lipophilicity, cell membrane permeability, and intracellular antioxidant capacity of resveratrol and pterostilbene

Pterostilbene (PTS), a methylated analog of resveratrol (RES), demonstrates superior bioavailability, potentially attributable to enhanced lipophilicity and cell membrane permeability compared to RES. However, this hypothesis lacked experimental validation. The objective of this study was to compare the lipophilicity, cell membrane permeability, and intracellular antioxidant capacity of RES and PTS. Lipophilicity was assessed via the shake-flask method, revealing higher lipophilicity for PTS than RES. Molecular dynamics simulations predicted stronger membrane permeability for PTS. Cellular uptake studies in IPEC-J2 cells and porcine myotubes using cyanine2-labeled RES (CY2-RES) and PTS (CY2-PTS) confirmed these predictions, with fluorescence microscopy and flow cytometry consistently demonstrating higher intracellular accumulation of CY2-PTS compared to CY2-RES. Furthermore, PTS exhibited stronger intracellular ROS-scavenging capacity than RES in IPEC-J2 cells and porcine myotubes. In conclusion, our study demonstrates that PTS exhibits superior lipophilicity, enhanced membrane permeability, and stronger intracellular antioxidant capacity compared to RES.

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来源期刊

Journal of Nutritional Biochemistry 医学-生化与分子生物学

CiteScore

9.50

自引率

3.60%

发文量

237

审稿时长

68 days

期刊介绍： Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.