Physicochemical and Antimicrobial Properties of Bioceramic Sealer Enhanced with Silver Nanoparticles: An in vitro Evaluation.

Aim: The aim of this study was to determine the impact of different nanoparticle concentrations with endodontic bioceramic sealer. It was assessed the combination by analyzing the correlation between the degree of conversion (DC) and antibacterial efficacy. And assess the penetration depth into the lateral canals.

Materials and methods: The AH Plus bioceramic sealers were mixed with silver nanoparticles (Ag) at different concentrations (2.5%, 5%, 10%). The antibacterial efficacy against Enterococcus faecalis was evaluated utilizing the direct contact test (DCT) at multiple time intervals (immediately, 30 minutes, 1 day, 2 days, and 7 days). The conversion degree was evaluated by FTIR. The penetration depth was evaluated using X-ray examination.

Results: The antibacterial activity increased with higher concentrations of nanoparticles, with silver nanoparticles demonstrating a pronounced effect on E. faecalis. The conversion degree increased with high quantities of silver nanoparticles. Positive, strong relation between the degree of conversion and antimicrobial activity. The penetration depth decreased as nanoparticle concentration increased. A statistically significant difference was seen between the antibacterial activity values and the degree of conversion at different time intervals (P = 0.05).

Conclusion: The highest nanoparticles concentration (10%) has the greatest effect on the antibacterial activity and degree of conversion, the highest nanoparticles concentration decreased the penetration depth into the lateral canals.