Mathieu Victor Paulin, Thomas Schermerhorn, Dorsa Mehrabanpour, Suraj Unniappan, Elisabeth Snead
{"title":"犬静息及经渗透和非渗透刺激试验后血浆精氨酸加压素和血清Copeptin浓度","authors":"Mathieu Victor Paulin, Thomas Schermerhorn, Dorsa Mehrabanpour, Suraj Unniappan, Elisabeth Snead","doi":"10.1111/jvim.70219","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Serum copeptin (sCoP) is used as a surrogate for plasma arginine vasopressin (pAVP) measurement in humans.</p>\n </section>\n \n <section>\n \n <h3> Objective</h3>\n \n <p>To measure pAVP and sCoP at rest and after osmotic- and non-osmotic stimulation testing in dogs.</p>\n </section>\n \n <section>\n \n <h3> Animals</h3>\n \n <p>Eight young castrated/spayed healthy research Beagles, eight young intact dogs, and eight old neutered healthy client-owned dogs.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>In this prospective longitudinal study, pAVP and sCoP were measured under iso-(baseline), hypo-(water load followed by intravenous administration of desmopressin [WLT]), and hyper-(water deprivation test [WDT]) osmolar conditions assessed by measured plasma osmolality (pOsm<sub>(m)</sub>), and after administration of arginine (AST), IV, and Bovril (BST), PO. The fraction of change (<i>F</i>) in a variable <i>y</i> (e.g., pAVP) between baseline (<i>T</i><sub>0</sub>) and a timepoint <i>X</i> (<i>T</i><sub><i>X</i></sub>) during testing was defined as <i>Fy</i> = [<i>y</i> (<i>T</i><sub><i>X</i></sub>) – <i>y</i> (<i>T</i><sub>0</sub>)]/<i>y</i> (<i>T</i><sub>0</sub>).</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Baseline sCoP had wide inter-individual variations. Mean [range] FpAVP and FsCoP at the end of WDT were +110% [+80; +142] and +18% [+0.4; +38] compared to baseline, respectively. Mean [range] FpAVP after water load and FsCoP after water load followed by desmopressin administration were −22% [−48; −0.5] and −29% [−39; −14] compared to baseline, respectively. Both FpAVP and FsCoP were strongly correlated to FpOsm<sub>(m)</sub> (<i>r</i> = +0.76, <i>p</i> = 0.004; <i>r</i> = +0.78, <i>p</i> = 0.002; respectively). When sCoP was measured at <i>T</i><sub>4h</sub> instead of <i>T</i><sub>2h</sub> during WLT, to reflect its longer half-life reported in humans, the correlation between FpAVP<sub>P800</sub> and FsCoP became excellent (<i>r</i> = +0.90, <i>p</i> < 0.001). No stimulation of sCoP secretion occurred during AST or BST.</p>\n </section>\n \n <section>\n \n <h3> Conclusion and Clinical Importance</h3>\n \n <p>Serum CoP could be used as a surrogate for pAVP measurement in healthy dogs.</p>\n </section>\n </div>","PeriodicalId":49958,"journal":{"name":"Journal of Veterinary Internal Medicine","volume":"39 5","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jvim.70219","citationCount":"0","resultStr":"{\"title\":\"Plasma Arginine Vasopressin and Serum Copeptin Concentrations at Rest and After Osmotic- and Non-Osmotic-Based Stimulation Tests in Dogs\",\"authors\":\"Mathieu Victor Paulin, Thomas Schermerhorn, Dorsa Mehrabanpour, Suraj Unniappan, Elisabeth Snead\",\"doi\":\"10.1111/jvim.70219\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Serum copeptin (sCoP) is used as a surrogate for plasma arginine vasopressin (pAVP) measurement in humans.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>To measure pAVP and sCoP at rest and after osmotic- and non-osmotic stimulation testing in dogs.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Animals</h3>\\n \\n <p>Eight young castrated/spayed healthy research Beagles, eight young intact dogs, and eight old neutered healthy client-owned dogs.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>In this prospective longitudinal study, pAVP and sCoP were measured under iso-(baseline), hypo-(water load followed by intravenous administration of desmopressin [WLT]), and hyper-(water deprivation test [WDT]) osmolar conditions assessed by measured plasma osmolality (pOsm<sub>(m)</sub>), and after administration of arginine (AST), IV, and Bovril (BST), PO. The fraction of change (<i>F</i>) in a variable <i>y</i> (e.g., pAVP) between baseline (<i>T</i><sub>0</sub>) and a timepoint <i>X</i> (<i>T</i><sub><i>X</i></sub>) during testing was defined as <i>Fy</i> = [<i>y</i> (<i>T</i><sub><i>X</i></sub>) – <i>y</i> (<i>T</i><sub>0</sub>)]/<i>y</i> (<i>T</i><sub>0</sub>).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Baseline sCoP had wide inter-individual variations. Mean [range] FpAVP and FsCoP at the end of WDT were +110% [+80; +142] and +18% [+0.4; +38] compared to baseline, respectively. Mean [range] FpAVP after water load and FsCoP after water load followed by desmopressin administration were −22% [−48; −0.5] and −29% [−39; −14] compared to baseline, respectively. Both FpAVP and FsCoP were strongly correlated to FpOsm<sub>(m)</sub> (<i>r</i> = +0.76, <i>p</i> = 0.004; <i>r</i> = +0.78, <i>p</i> = 0.002; respectively). When sCoP was measured at <i>T</i><sub>4h</sub> instead of <i>T</i><sub>2h</sub> during WLT, to reflect its longer half-life reported in humans, the correlation between FpAVP<sub>P800</sub> and FsCoP became excellent (<i>r</i> = +0.90, <i>p</i> < 0.001). No stimulation of sCoP secretion occurred during AST or BST.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion and Clinical Importance</h3>\\n \\n <p>Serum CoP could be used as a surrogate for pAVP measurement in healthy dogs.</p>\\n </section>\\n </div>\",\"PeriodicalId\":49958,\"journal\":{\"name\":\"Journal of Veterinary Internal Medicine\",\"volume\":\"39 5\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-09-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jvim.70219\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Internal Medicine\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jvim.70219\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Internal Medicine","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jvim.70219","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
背景：血清copeptin （sCoP）被用作人类血浆精氨酸抗利尿激素（pAVP）测量的替代品。目的测定犬在静息状态及经渗透和非渗透刺激试验后的pAVP和sCoP。8只被阉割/绝育的健康小猎犬，8只完整的小猎犬，8只被阉割的健康客户犬。方法在这项前瞻性纵向研究中，在等（基线）、低（水负荷后静脉给药去氨加压素[WLT]）和高（水剥夺试验[WDT]）渗透压条件下测量pAVP和sCoP，通过测量血浆渗透压（pOsm(m)）以及给药精氨酸（AST）、IV和Bovril （BST）、PO后评估。在测试期间，基线（T0）和时间点X （TX）之间的变量y（例如，pAVP）的变化(F)的分数定义为Fy = [y (TX) - y (T0)]/y （T0）。结果基线sCoP存在较大的个体间差异。WDT结束时FpAVP和FsCoP平均[范围]为+110% [+80]；+142]和+18% [+0.4；+38]分别与基线相比。平均[范围]水负荷后的FpAVP和水负荷后去氨加压素的FsCoP为- 22% [- 48]；−0.5]和−29%[−39；−14]，分别与基线相比。FpAVP和FsCoP与FpOsm(m)呈显著正相关（r = +0.76, p = 0.004; r = +0.78, p = 0.002）。当在WLT期间的第4小时而不是第2小时测量sCoP时，为了反映其在人类中报道的更长的半衰期，FpAVPP800与FsCoP之间的相关性变得很好（r = +0.90, p < 0.001）。在AST和BST期间没有sCoP分泌的刺激。结论及临床意义血清CoP可作为健康犬体内pap测定的替代指标。
Plasma Arginine Vasopressin and Serum Copeptin Concentrations at Rest and After Osmotic- and Non-Osmotic-Based Stimulation Tests in Dogs
Background
Serum copeptin (sCoP) is used as a surrogate for plasma arginine vasopressin (pAVP) measurement in humans.
Objective
To measure pAVP and sCoP at rest and after osmotic- and non-osmotic stimulation testing in dogs.
Animals
Eight young castrated/spayed healthy research Beagles, eight young intact dogs, and eight old neutered healthy client-owned dogs.
Methods
In this prospective longitudinal study, pAVP and sCoP were measured under iso-(baseline), hypo-(water load followed by intravenous administration of desmopressin [WLT]), and hyper-(water deprivation test [WDT]) osmolar conditions assessed by measured plasma osmolality (pOsm(m)), and after administration of arginine (AST), IV, and Bovril (BST), PO. The fraction of change (F) in a variable y (e.g., pAVP) between baseline (T0) and a timepoint X (TX) during testing was defined as Fy = [y (TX) – y (T0)]/y (T0).
Results
Baseline sCoP had wide inter-individual variations. Mean [range] FpAVP and FsCoP at the end of WDT were +110% [+80; +142] and +18% [+0.4; +38] compared to baseline, respectively. Mean [range] FpAVP after water load and FsCoP after water load followed by desmopressin administration were −22% [−48; −0.5] and −29% [−39; −14] compared to baseline, respectively. Both FpAVP and FsCoP were strongly correlated to FpOsm(m) (r = +0.76, p = 0.004; r = +0.78, p = 0.002; respectively). When sCoP was measured at T4h instead of T2h during WLT, to reflect its longer half-life reported in humans, the correlation between FpAVPP800 and FsCoP became excellent (r = +0.90, p < 0.001). No stimulation of sCoP secretion occurred during AST or BST.
Conclusion and Clinical Importance
Serum CoP could be used as a surrogate for pAVP measurement in healthy dogs.
期刊介绍：
The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
