Yanke Zhai, Dan Wang, Qianqing Lin, Ruixin Yang, Guo Chen, Xiaotong Li, Haonan Jin, Hualu Zhang, Yibing Ji, Ruijun Li
{"title":"基于级联系统的高活性自组装纳米酶用于肌氨酸双模检测","authors":"Yanke Zhai, Dan Wang, Qianqing Lin, Ruixin Yang, Guo Chen, Xiaotong Li, Haonan Jin, Hualu Zhang, Yibing Ji, Ruijun Li","doi":"10.1007/s00604-025-07501-6","DOIUrl":null,"url":null,"abstract":"<div><p>A nanozyme-mediated cascade reaction system for fluorometric and colorimetric dual-mode detection of sarcosine (SA) was developed. The nanozymes (Zn-Glu@Hemin) were synthesized via a rapid self-assembly within 10 min at room temperature. Importantly, the Zn-Glu@Hemin exhibited strong peroxidase (POD)-mimicking activity, catalyzing the generation of hydroxyl radical (·OH) and superoxide anion (O<sub>2</sub><sup>·−</sup>) from hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), enhancing the fluorescence reaction of o-phenylenediamine (OPD) and the colorimetric reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). Thus, a dual-mode sensing platform is created by combining SA and sarcosine oxidase (SOD) into the enzyme cascade system (SA-SOD-POD). The developed sensors exhibited superior performance, with high sensitivity and selectivity for SA detection. The detection limits were 0.14 μM (fluorescence) and 0.26 μM (colorimetric), with detection ranges of 1–120 μM and 1–70 μM, respectively. The system was incorporated into both a smartphone-assisted dual-signal sensing platform and a portable field detection kit, demonstrating practical applicability. Besides, the practicability of the established approach was confirmed in human serum samples with reliable recoveries. This work shows advantages in the synthesis method and detection sensitivity of nanozymes, which can accurately detect disease biomarkers and show high potential in real-time diagnosis.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A highly active self-assembled nanozyme based on cascade system for dual-mode detection of sarcosine\",\"authors\":\"Yanke Zhai, Dan Wang, Qianqing Lin, Ruixin Yang, Guo Chen, Xiaotong Li, Haonan Jin, Hualu Zhang, Yibing Ji, Ruijun Li\",\"doi\":\"10.1007/s00604-025-07501-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A nanozyme-mediated cascade reaction system for fluorometric and colorimetric dual-mode detection of sarcosine (SA) was developed. The nanozymes (Zn-Glu@Hemin) were synthesized via a rapid self-assembly within 10 min at room temperature. Importantly, the Zn-Glu@Hemin exhibited strong peroxidase (POD)-mimicking activity, catalyzing the generation of hydroxyl radical (·OH) and superoxide anion (O<sub>2</sub><sup>·−</sup>) from hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), enhancing the fluorescence reaction of o-phenylenediamine (OPD) and the colorimetric reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). Thus, a dual-mode sensing platform is created by combining SA and sarcosine oxidase (SOD) into the enzyme cascade system (SA-SOD-POD). The developed sensors exhibited superior performance, with high sensitivity and selectivity for SA detection. The detection limits were 0.14 μM (fluorescence) and 0.26 μM (colorimetric), with detection ranges of 1–120 μM and 1–70 μM, respectively. The system was incorporated into both a smartphone-assisted dual-signal sensing platform and a portable field detection kit, demonstrating practical applicability. Besides, the practicability of the established approach was confirmed in human serum samples with reliable recoveries. This work shows advantages in the synthesis method and detection sensitivity of nanozymes, which can accurately detect disease biomarkers and show high potential in real-time diagnosis.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 10\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-09-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-07501-6\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07501-6","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A highly active self-assembled nanozyme based on cascade system for dual-mode detection of sarcosine
A nanozyme-mediated cascade reaction system for fluorometric and colorimetric dual-mode detection of sarcosine (SA) was developed. The nanozymes (Zn-Glu@Hemin) were synthesized via a rapid self-assembly within 10 min at room temperature. Importantly, the Zn-Glu@Hemin exhibited strong peroxidase (POD)-mimicking activity, catalyzing the generation of hydroxyl radical (·OH) and superoxide anion (O2·−) from hydrogen peroxide (H2O2), enhancing the fluorescence reaction of o-phenylenediamine (OPD) and the colorimetric reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). Thus, a dual-mode sensing platform is created by combining SA and sarcosine oxidase (SOD) into the enzyme cascade system (SA-SOD-POD). The developed sensors exhibited superior performance, with high sensitivity and selectivity for SA detection. The detection limits were 0.14 μM (fluorescence) and 0.26 μM (colorimetric), with detection ranges of 1–120 μM and 1–70 μM, respectively. The system was incorporated into both a smartphone-assisted dual-signal sensing platform and a portable field detection kit, demonstrating practical applicability. Besides, the practicability of the established approach was confirmed in human serum samples with reliable recoveries. This work shows advantages in the synthesis method and detection sensitivity of nanozymes, which can accurately detect disease biomarkers and show high potential in real-time diagnosis.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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