睾丸特异性丝氨酸/苏氨酸激酶3调节斯氏按蚊精子库的大小。

IF 2.1 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Keshav Kumar Meghwanshi, Chhavi Choudhary, Pooja Rohilla, Rajnikant Dixit, Vishal Saxena, Jayendra Nath Shukla
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引用次数: 0

摘要

蚊子生殖生物学是一个未充分开发的领域,具有开发新的媒介控制策略的潜力。在这项研究中,我们研究了睾丸特异性丝氨酸/苏氨酸蛋白激酶(tssk)家族在蚊子中的作用,tssk家族是哺乳动物精子发生的重要调节因子。我们分别在斯氏按蚊和埃及伊蚊中鉴定出tssk同源基因As_tssk3和Aea_tssk1,并分析了它们在不同发育阶段的表达情况。通过RNAi介导的基因沉默,对An中As_tssk3的功能进行了表征。stephensi。采用qRT-PCR分析As_tssk3基因敲低的效率,并比较敲低雄性和对照雄性睾丸的表型变化。此外,通过交配分析分析As_tssk3基因敲除对雄蚊生殖潜能的影响。此外,我们还进行了计算机研究,以确定As_TSSK3与按蚊精子发生过程中已知的果蝇TSSK靶点的同源物之间的相互作用。As_tssk3和Aea_tssk1在整个发育过程中以男性特有的方式表达。饲喂An后,As_tssk3基因的表达有效下调。带有目标dsRNA的史蒂芬氏蝇幼虫。As_tssk3基因的敲除导致了成年男性精子库的减少。As_TSSK3被发现与核小体组装蛋白1like-4 (NAP1like-4)相互作用,NAP1like-4是我们在计算机分析中推测的转录因子。我们推测As_TSSK3可能通过NAP蛋白调控鱼精蛋白基因的转录。这项研究首次报道了蚊子中tssk3同源物的特征,并证明了As_tssk3在维持精子储存方面的可能作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Testis-specific serine/threonine kinase 3 regulates the size of sperm reservoir in Anopheles stephensi.

Mosquito reproductive biology is an underexplored area with potential for developing novel vector control strategies. In this study, we investigated the role of the testis-specific serine/threonine-protein kinase (tssk) family, an essential regulator of spermiogenesis in mammals, in mosquitoes. We identified tssk homologues, As_tssk3 and Aea_tssk1, in Anopheles stephensi and Aedes aegypti, respectively and analyzed their expression across different developmental stages. Functional characterization of As_tssk3 was performed by RNAi mediated gene silencing in An. stephensi. The efficiency of As_tssk3 knockdown was analyzed using qRT-PCR, and the testes of knockdown and control males were compared for phenotypic changes. Additionally, the effect of As_tssk3 knockdown on the reproductive potential of male mosquitoes was analyzed via mating analysis. Further, in-silico studies were conducted to identify interactions between As_TSSK3 and the homologues of known Drosophila TSSK targets involved in the process of spermatogenesis, in Anopheles. As_tssk3 and Aea_tssk1 were found to express in a male-specific manner throughout development. Efficient knockdown in the expression of As_tssk3 gene was observed upon feeding An. stephensi larvae with target dsRNA. The knockdown of As_tssk3 led to a reduced sperm reservoir in adult males. As_TSSK3 was found to interact with the Nucleosome Assembly Protein1 like-4 (NAP1like-4), a putative transcription factor in our in-silico analysis. We hypothesize that As_TSSK3 might regulate the transcription of the protamine gene via the NAP protein. The study presented here is the first report of the characterization of tssk homologues in mosquitoes' and demonstrates a possible role of As_tssk3 in maintaining sperm stores.

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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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