RNA解旋酶CgDBP4提高了产甘油假丝酵母和解脂耶氏菌对未解毒纤维素水解产物的耐受性和发酵性能

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-08-29 DOI:10.1016/j.bej.2025.109919

Xinyao Lu , Xiaoqing Hao , Xinran Mao , Bin Zhuge , Hong Zong

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引用次数: 0

摘要

RNA解旋酶在RNA转录、翻译和降解中起作用，促进mRNP的组装，影响细胞的糖转运过程、DNA修复和细胞周期进程。本研究利用产甘油假丝酵母的RNA解旋酶CgDBP4作为产甘油假丝酵母和脂肪瘤假丝酵母的抗逆性遗传元件。CgDBP4影响C甘油原细胞对NaCl、高糖、醋酸和热胁迫的耐受性。在44℃下，C. g- antidbp4（一种适度上调CgDBP4转录水平的重组菌株）的产乙醇率从1.5 g·（L·h⁻¹）增加到2.0 g·（L·h⁻¹）。CgTy3是一种逆转录转座子，可在热应激下挽救c - g抗dbp4的细胞生长。CgTy3的下调使RNA聚合酶III的转录、基因组稳定性、蛋白质加工和热响应基因的激活受损，从而影响CgDBP4对细胞热应激的耐受能力。CgDBP4还增强了脂质体Y. polytica Po1f对多种胁迫的抗性，在典型培养基中脂质滴度提高了44.5 %。此外，Y. l‐CgDBP4在34℃下产生38.1 mg/ l的脂质，在未解毒的纤维素水解物中产生49.1 mg/ l的脂质。

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RNA helicase CgDBP4 improves tolerance and fermentation performance of Candida glycerinogenes and Yarrowia lipolytica from undetoxified cellulose hydrolysate

RNA helicase functions in RNA transcription, translation, and degradation that promotes the assembly of mRNP, affecting the cell's sugar transport process, DNA repair, and cell cycle progression. This study employed the RNA helicase CgDBP4 from Candida glycerinogenes as a stress-resistant genetic element for both C. glycerinogenes and Y. lipolytica. CgDBP4 affected cell tolerance of C glycerinogenes to NaCl, high sugar, acetic acid, and heat stresses. Under 44 ℃, the ethanol production rate of C. g-antiDBP4 (It is a recombinant strain that moderately upregulates the transcriptional level of CgDBP4) increased from 1.5 g·(L·h⁻¹) to 2.0 g·(L·h⁻¹). CgTy3 is a retrotransposon rescued the cell growth of the C. g-antiDBP4 under heat stress. The downregulation of CgTy3 impaired the transcription of RNA polymerase III, genomic stability, protein processing, and the activation of heat-responsive genes, consequently influencing the ability of CgDBP4 in cell tolerate to heat stress. CgDBP4 also enhanced the resistance of Y. lipolytica Po1f to multiple stresses and improved lipid titer by 44.5 % in typical media. Besides, Y. l‐CgDBP4 produced 38.1 mg/L of lipids under 34 ℃ and 49.1 mg/L of lipids in undetoxified cellulose hydrolysates.

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.