Grant Johnson, Valentin Parvu, Stephane Beauchamp, Mike Cuttler, Arek Zubrzycki, Lauren Cooper
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Differences in average fill volume between pre- and post-conversion were evaluated overall and by month. A separate logistic regression was also performed to model the relationship between monthly average blood volume increase (mL) and sample positivity rate. Prevalence rates for identified organisms were also assessed. The blood fill volume of 13,356 pre-conversion and 119,971 post-conversion bottles was compared. The average post-conversion fill volume was significantly higher than that of pre-conversion (7.56 mL [95% CI, 7.54-7.59] vs. 5.68 mL [95% CI, 5.62-5.74], respectively). This 1.88 mL increase in collected blood volume increased sample positivity from 7.0% to 7.7% (<i>P</i> = 0.002; odds ratio [OR], 1.11; 95% CI, 1.04-1.19). This study demonstrated that using an ultrathin-wall cannula blood collection device can significantly increase blood culture volume and sample positivity, supporting improved identification of patients suffering from bloodstream infections.IMPORTANCESubstandard blood fill volume practices in clinical settings negatively affect pathogen recovery which, in turn, may impact patients' care. Whereas professional education regarding the importance of adhering to clinical guidelines has been helpful in partly remediating the issue, the role of blood collection set technologies in optimizing blood culture volume has not been studied as thoroughly. 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The blood fill volume of 13,356 pre-conversion and 119,971 post-conversion bottles was compared. The average post-conversion fill volume was significantly higher than that of pre-conversion (7.56 mL [95% CI, 7.54-7.59] vs. 5.68 mL [95% CI, 5.62-5.74], respectively). This 1.88 mL increase in collected blood volume increased sample positivity from 7.0% to 7.7% (<i>P</i> = 0.002; odds ratio [OR], 1.11; 95% CI, 1.04-1.19). This study demonstrated that using an ultrathin-wall cannula blood collection device can significantly increase blood culture volume and sample positivity, supporting improved identification of patients suffering from bloodstream infections.IMPORTANCESubstandard blood fill volume practices in clinical settings negatively affect pathogen recovery which, in turn, may impact patients' care. Whereas professional education regarding the importance of adhering to clinical guidelines has been helpful in partly remediating the issue, the role of blood collection set technologies in optimizing blood culture volume has not been studied as thoroughly. 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引用次数: 0
摘要
在临床血培养样本中,不理想的血容量采集对病原体的恢复和病原体检测时间产生负面影响。本研究评估了BD Vacutainer UltraTouch按钮式采血装置(UltraTouch; BD Life Sciences, Franklin Lakes, NJ)的超薄壁套管装置与常规套管相比在增加采血量方面的性能。在这项回顾性研究中,在盲转到UltraTouch之前和之后,在BD BACTEC FX仪器中使用定制的软件应用程序(正在申请专利),根据初始孵育期间获得的血液背景代谢活动信号,测量阴性BD BACTEC Plus有氧/F培养小管中采集的血液体积。转换前和转换后的平均填充量的差异进行了总体和月度评估。对月平均血容量增加(mL)与样本阳性率之间的关系也进行了单独的logistic回归。还评估了已确定生物体的流行率。比较了转化前13356瓶和转化后119971瓶的血填充量。转换后的平均填充量显著高于转换前(分别为7.56 mL [95% CI, 7.54-7.59]和5.68 mL [95% CI, 5.62-5.74])。收集的血容量增加1.88 mL,使样本阳性率从7.0%增加到7.7% (P = 0.002;优势比[OR], 1.11; 95% CI, 1.04-1.19)。本研究表明,使用超薄壁套管采血装置可以显著增加血培养量和样本阳性率,有助于提高对血流感染患者的识别。重要性临床环境中不合标准的血填充量做法会对病原体的恢复产生负面影响,进而可能影响患者的护理。尽管关于坚持临床指南重要性的专业教育在一定程度上有助于解决这一问题,但采血装置技术在优化血培养量方面的作用尚未得到彻底研究。我们的研究评估了BD Vacutainer UltraTouch按钮采血装置的性能,该装置设计了一个超薄壁套管,以提高血培养量。
Enhancing blood culture volume with ultrathin-wall cannula devices.
Suboptimal blood volume collection negatively affects pathogen recovery and time to pathogen detection in clinical blood culture samples. This study evaluated the performance of the ultrathin-wall cannula device of the BD Vacutainer UltraTouch Push Button Blood Collection Set (UltraTouch; BD Life Sciences, Franklin Lakes, NJ) in increasing the blood volume collected compared to regular cannulas. In this retrospective study, the volume of blood collected in negative BD BACTEC Plus Aerobic/F Culture Vials prior to and following blind conversion to UltraTouch was measured from blood background metabolic activity signal acquired during the initial incubation period in the BD BACTEC FX instrument using a custom software application (patent pending). Differences in average fill volume between pre- and post-conversion were evaluated overall and by month. A separate logistic regression was also performed to model the relationship between monthly average blood volume increase (mL) and sample positivity rate. Prevalence rates for identified organisms were also assessed. The blood fill volume of 13,356 pre-conversion and 119,971 post-conversion bottles was compared. The average post-conversion fill volume was significantly higher than that of pre-conversion (7.56 mL [95% CI, 7.54-7.59] vs. 5.68 mL [95% CI, 5.62-5.74], respectively). This 1.88 mL increase in collected blood volume increased sample positivity from 7.0% to 7.7% (P = 0.002; odds ratio [OR], 1.11; 95% CI, 1.04-1.19). This study demonstrated that using an ultrathin-wall cannula blood collection device can significantly increase blood culture volume and sample positivity, supporting improved identification of patients suffering from bloodstream infections.IMPORTANCESubstandard blood fill volume practices in clinical settings negatively affect pathogen recovery which, in turn, may impact patients' care. Whereas professional education regarding the importance of adhering to clinical guidelines has been helpful in partly remediating the issue, the role of blood collection set technologies in optimizing blood culture volume has not been studied as thoroughly. Our study assessed the performance of the BD Vacutainer UltraTouch Push Button Blood Collection Set, which is designed with an ultrathin-wall cannula, to improve blood culture volume.
期刊介绍:
The Journal of Clinical Microbiology® disseminates the latest research concerning the laboratory diagnosis of human and animal infections, along with the laboratory's role in epidemiology and the management of infectious diseases.