Przemyslaw Mielczarek, Kinga Hartman, Eagle Yi-Kung Huang, Ewa Gibula-Tarlowska, Pawel Grochecki, Tymoteusz Slowik, Jolanta H. Kotlinska, Jerzy Silberring, Anna Drabik
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The Role of LVV-H7 in Alcohol-Induced Reward Mechanisms
This study aimed to investigate the role of LVV-hemorphin-7 (LVV-H7) in alcohol dependence. LVV-H7 is a short peptide derived from the cleavage of haemoglobin chains that binds to opioid receptors and plays diverse roles in various physiological and pathological processes. Additionally, LVV-H7 is cleaved at higher concentrations in the presence of alcohol. We conducted behavioural experiments in animal models and performed proteomic analyses of CNS tissues from alcohol-addicted rats to identify LVV-H7 binding partners. Using fluorescent microscopy, we confirmed the blood–brain barrier (BBB) permeability of synthesized LVV-H7 and its releasing enzyme inhibitor, pepstatin. Our results revealed a dose-dependent correlation between LVV-H7 quantities and alcohol levels. Mass spectrometry-based analyses identified LVV-H7's protein-binding targets in CNS tissues of addicted rats and the enzymes responsible for their degradation. These findings highlight the significant role of LVV-H7 in the mechanisms underlying alcohol dependence and indicate the potential role of hemorphin as a therapeutic target.
期刊介绍：
Addiction Biology is focused on neuroscience contributions and it aims to advance our understanding of the action of drugs of abuse and addictive processes. Papers are accepted in both animal experimentation or clinical research. The content is geared towards behavioral, molecular, genetic, biochemical, neuro-biological and pharmacology aspects of these fields.
Addiction Biology includes peer-reviewed original research reports and reviews.
Addiction Biology is published on behalf of the Society for the Study of Addiction to Alcohol and other Drugs (SSA). Members of the Society for the Study of Addiction receive the Journal as part of their annual membership subscription.
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