脊髓非靶向代谢组学揭示银针热疗治疗肌筋膜疼痛综合征的镇痛机制

IF 2.5 4区综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES

Journal of Radiation Research and Applied Sciences Pub Date : 2025-09-04 DOI:10.1016/j.jrras.2025.101923

Junlong Yang , Xianglong Lv , Yunting Chen , Changyu Shen , Huaqiao Chen , Lin Wang , Jing Yao , Yuanxin Huang

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引用次数: 0

摘要

目的利用非靶向代谢组学方法探讨银针热疗治疗肌筋膜疼痛综合征（MPS）的脊柱镇痛机制。方法采用钝器撞击法和平板运动法建立MPS大鼠模型。将大鼠分为正常组(N)、模型组(M)和银针热疗组(S)。通过机械戒断阈值（MWT）评估疼痛敏感性。肌电图（EMG）记录MTrP活性，苏木精-伊红（HE）染色评估肌肉病理，免疫荧光定量测定脊髓降钙素基因相关肽（CGRP）和P物质（SP）的表达。采用液相色谱串联质谱法（LC-MS/MS）对脊髓组织进行非靶向代谢组学分析。结果与M组比较，S组MWT显著升高（P < 0.01），肌电信号归一化，肌肉组织病理学改善。银针热疗可下调脊髓CGRP和SP的表达（P < 0.05）。代谢组学鉴定出脊髓组织中的52种差异代谢物。富集分析揭示了三个关键通路的参与：2-氧羧酸代谢（柠檬酸和3-甲基-2-氧丁酸的上调；n -乙酰氨基氨酸的下调），细胞色素p450介导的外源代谢（尼古丁的上调）和淀粉/蔗糖代谢（d -果糖6-磷酸的下调）。结论银针热疗通过调节与神经元兴奋性、神经炎症和能量调节相关的脊柱代谢通路来缓解MPS。非靶向代谢组学有效地揭示了这些脊柱机制，促进了对MPS病理生理和银针镇痛的理解。

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Untargeted metabolomics of spinal cord reveals analgesic mechanism of silver-needle thermotherapy for myofascial pain syndrome

Objective

To elucidate the spinal analgesic mechanism of silver-needle thermotherapy in myofascial pain syndrome (MPS) using untargeted metabolomics.

Methods

An MPS rat model was established by blunt impact and treadmill exercise. Rats were divided into normal (N), model (M), and silver-needle thermotherapy (S) groups. Pain sensitivity was assessed via mechanical withdrawal threshold (MWT). Electromyography (EMG) recorded MTrP activity, hematoxylin-eosin (HE) staining evaluated muscle pathology, and immunofluorescence quantified spinal calcitonin gene-related peptide (CGRP) and substance P (SP) expression. Untargeted metabolomics of spinal cord tissue was performed using liquid chromatography tandem mass spectrometry (LC-MS/MS).

Results

Compared to group M, group S showed significantly increased MWT (P < 0.01), normalized EMG signals, and improved muscle histopathology. Silver-needle thermotherapy downregulated spinal CGRP and SP expression (P < 0.05). Metabolomics identified 52 differential metabolites in spinal cord tissue. Enrichment analysis revealed involvement of three key pathways: 2-oxocarboxylic acid metabolism (upregulated citric acid and 3-methyl-2-oxobutanoic acid; downregulated N-acetylornithine), cytochrome P450-mediated xenobiotic metabolism (upregulated nicotine), and starch/sucrose metabolism (downregulated D-fructose 6-phosphate).

Conclusion

Silver-needle thermotherapy alleviates MPS by modulating spinal metabolic pathways linked to neuronal excitability, neuroinflammation, and energy regulation. Untargeted metabolomics effectively uncovered these spinal mechanisms, advancing understanding of MPS pathophysiology and silver-needle analgesia.

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来源期刊

Journal of Radiation Research and Applied Sciences MULTIDISCIPLINARY SCIENCES-

自引率

5.90%

发文量

130

审稿时长

16 weeks

期刊介绍： Journal of Radiation Research and Applied Sciences provides a high quality medium for the publication of substantial, original and scientific and technological papers on the development and applications of nuclear, radiation and isotopes in biology, medicine, drugs, biochemistry, microbiology, agriculture, entomology, food technology, chemistry, physics, solid states, engineering, environmental and applied sciences.