Andrew T E Hartwick, Christina E Morettin, Jennifer S Harthan, Meredith Whiteside, Ellen Shorter, Spencer D Johnson, Mary K Migneco, Christian K Olson, Julia B Huecker, Tammy Than, Mae O Gordon
{"title":"结膜炎恢复期患者结膜标本中人疱疹病毒7型的检测。","authors":"Andrew T E Hartwick, Christina E Morettin, Jennifer S Harthan, Meredith Whiteside, Ellen Shorter, Spencer D Johnson, Mary K Migneco, Christian K Olson, Julia B Huecker, Tammy Than, Mae O Gordon","doi":"10.3389/fopht.2025.1641991","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Although it is often reported that adenovirus is the most common etiology for infectious conjunctivitis, a recent multi-center clinical study found that adenovirus was confirmed by polymerase chain reaction in only 16% of cases presenting with acute conjunctivitis. Here, we investigated the hypothesis that a member of Herpesviridae could be the underlying etiology in some non-adenoviral cases of conjunctivitis.</p><p><strong>Methods: </strong>Molecular assays for Herpes Simplex 1 and 2 (HSV-1, HSV-2) and Human Herpesvirus 6A, 6B and 7 (HHV-6A, HHV-6B, HHV-7) were performed on conjunctival samples collected from 18 individuals with acute conjunctivitis and during their recovery in follow-up visits that spanned up to 3 weeks. All samples, obtained from individuals enrolled in a clinical trial evaluating a conjunctivitis treatment, were from eyes that had previously tested negative for adenovirus using polymerase chain reaction (PCR) techniques.</p><p><strong>Results: </strong>In total, 160 PCR assays were performed on 40 conjunctival samples. Four of these samples, obtained from four different individuals, tested positive for HHV-7. None of the samples tested positive for HSV-1, HSV-2, HHV-6A or HHV-6B.</p><p><strong>Conclusion: </strong>This data provides further evidence that Human Herpesvirus 7 can be present in the eye, as HHV-7 was detected in a subset of conjunctival samples obtained from individuals recovering from non-adenoviral conjunctivitis. Clinicians should consider non-adenoviral etiologies when managing conjunctivitis that presents as classic 'pink eye'.</p>","PeriodicalId":73096,"journal":{"name":"Frontiers in ophthalmology","volume":"5 ","pages":"1641991"},"PeriodicalIF":0.9000,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401696/pdf/","citationCount":"0","resultStr":"{\"title\":\"Detection of human herpesvirus 7 in conjunctival samples collected from individuals recovering from conjunctivitis.\",\"authors\":\"Andrew T E Hartwick, Christina E Morettin, Jennifer S Harthan, Meredith Whiteside, Ellen Shorter, Spencer D Johnson, Mary K Migneco, Christian K Olson, Julia B Huecker, Tammy Than, Mae O Gordon\",\"doi\":\"10.3389/fopht.2025.1641991\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Although it is often reported that adenovirus is the most common etiology for infectious conjunctivitis, a recent multi-center clinical study found that adenovirus was confirmed by polymerase chain reaction in only 16% of cases presenting with acute conjunctivitis. Here, we investigated the hypothesis that a member of Herpesviridae could be the underlying etiology in some non-adenoviral cases of conjunctivitis.</p><p><strong>Methods: </strong>Molecular assays for Herpes Simplex 1 and 2 (HSV-1, HSV-2) and Human Herpesvirus 6A, 6B and 7 (HHV-6A, HHV-6B, HHV-7) were performed on conjunctival samples collected from 18 individuals with acute conjunctivitis and during their recovery in follow-up visits that spanned up to 3 weeks. All samples, obtained from individuals enrolled in a clinical trial evaluating a conjunctivitis treatment, were from eyes that had previously tested negative for adenovirus using polymerase chain reaction (PCR) techniques.</p><p><strong>Results: </strong>In total, 160 PCR assays were performed on 40 conjunctival samples. Four of these samples, obtained from four different individuals, tested positive for HHV-7. None of the samples tested positive for HSV-1, HSV-2, HHV-6A or HHV-6B.</p><p><strong>Conclusion: </strong>This data provides further evidence that Human Herpesvirus 7 can be present in the eye, as HHV-7 was detected in a subset of conjunctival samples obtained from individuals recovering from non-adenoviral conjunctivitis. Clinicians should consider non-adenoviral etiologies when managing conjunctivitis that presents as classic 'pink eye'.</p>\",\"PeriodicalId\":73096,\"journal\":{\"name\":\"Frontiers in ophthalmology\",\"volume\":\"5 \",\"pages\":\"1641991\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2025-08-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401696/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in ophthalmology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3389/fopht.2025.1641991\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in ophthalmology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3389/fopht.2025.1641991","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Detection of human herpesvirus 7 in conjunctival samples collected from individuals recovering from conjunctivitis.
Purpose: Although it is often reported that adenovirus is the most common etiology for infectious conjunctivitis, a recent multi-center clinical study found that adenovirus was confirmed by polymerase chain reaction in only 16% of cases presenting with acute conjunctivitis. Here, we investigated the hypothesis that a member of Herpesviridae could be the underlying etiology in some non-adenoviral cases of conjunctivitis.
Methods: Molecular assays for Herpes Simplex 1 and 2 (HSV-1, HSV-2) and Human Herpesvirus 6A, 6B and 7 (HHV-6A, HHV-6B, HHV-7) were performed on conjunctival samples collected from 18 individuals with acute conjunctivitis and during their recovery in follow-up visits that spanned up to 3 weeks. All samples, obtained from individuals enrolled in a clinical trial evaluating a conjunctivitis treatment, were from eyes that had previously tested negative for adenovirus using polymerase chain reaction (PCR) techniques.
Results: In total, 160 PCR assays were performed on 40 conjunctival samples. Four of these samples, obtained from four different individuals, tested positive for HHV-7. None of the samples tested positive for HSV-1, HSV-2, HHV-6A or HHV-6B.
Conclusion: This data provides further evidence that Human Herpesvirus 7 can be present in the eye, as HHV-7 was detected in a subset of conjunctival samples obtained from individuals recovering from non-adenoviral conjunctivitis. Clinicians should consider non-adenoviral etiologies when managing conjunctivitis that presents as classic 'pink eye'.