系统地研究和鉴定人类肠道微生物群中的细菌素。

IF 11.1 Q1 CELL BIOLOGY
Dengwei Zhang, Yinai Zou, Yuqi Shi, Junliang Zhang, Jing Liu, Gengfan Wu, Jian Zhang, Ying Gao, Muxuan Chen, Yong-Xin Li
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引用次数: 0

摘要

人类肠道微生物群产生未经修饰的细菌素,天然抗菌肽,保护病原体和调节宿主生理。然而,现有的生物信息学工具限制了对细菌素生物合成的全面研究,阻碍了对其生物学功能的研究。在这里,我们介绍IIBacFinder,一种用于鉴定未修饰的II类细菌素的优越分析管道。通过大规模的生物信息学分析和实验验证,我们证明了它们在细菌王国中广泛分布,并且大多数是特定于栖息地的。通过分析超过28万个细菌基因组,我们揭示了人类肠道细菌产生这些细菌素的多种潜力。在meta组学分析的指导下,我们从肠道共生物种中合成了26种假设的细菌素,其中16种具有抗菌活性。进一步的离体试验表明,窄谱细菌素对人类粪便微生物群的影响最小。我们的研究强调了未经修饰的细菌素在人类肠道中的巨大生物合成潜力,为理解其生物学功能和健康影响铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Systematically investigating and identifying bacteriocins in the human gut microbiome.

Human gut microbiota produces unmodified bacteriocins, natural antimicrobial peptides that protect against pathogens and regulate host physiology. However, current bioinformatic tools limit the comprehensive investigation of bacteriocins' biosynthesis, obstructing research into their biological functions. Here, we introduce IIBacFinder, a superior analysis pipeline for identifying unmodified class II bacteriocins. Through large-scale bioinformatic analysis and experimental validation, we demonstrate their widespread distribution across the bacterial kingdom, with most being habitat specific. Analyzing over 280,000 bacterial genomes, we reveal the diverse potential of human gut bacteria to produce these bacteriocins. Guided by meta-omics analysis, we synthesized 26 hypothetical bacteriocins from gut commensal species, with 16 showing antibacterial activities. Further ex vivo tests show minimal impact of narrow-spectrum bacteriocins on human fecal microbiota. Our study highlights the huge biosynthetic potential of unmodified bacteriocins in the human gut, paving the way for understanding their biological functions and health implications.

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